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Manual_CO-311 JBScreen Solubility HTS
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/SOLUB.pdf19 Mar 2018: In addition, both screens use equal ratio of protein and test condition, thereby making the effect of the buffer components in which the protein is stored more dominant. ... Finally, because, the screen tests for buffer, pH, salt and glycerol at the same -
J. Mol. Biol. (1974) 87, 641-656 Electron Microscopy of ...
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jmb74.pdf26 Jun 2024: It was therefore considered essential to subject the data to a mueh more stringent test, such as demonstrating a reasonable con- sistency in structure factors from one particle to the next. ... Other putative regions of departure from a-fold symmetry, -
Keith_Ligand_Meeting_Dec2016
https://www2.mrc-lmb.cam.ac.uk/coot/ligands/2016/Keith_Ligand_Meeting_Dec2016.pdf9 Dec 2016: Latest ligand test 1: i21. Make ligand in i2 from Smiles string using AceDRG. ... from the PDB? Latest ligand test 1: i2. N is para not meta? -
Paul EmsleyMRC Laboratory of Molecular Biology, Cambridge, UKJune…
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/Ligands-July-2015-v2.pdf16 Jul 2015: Can today's Acedrg fill the gaps? Acedrg Tests. Take the wwPDB Chemical Component Dictionary. ... Test vs Mogul worst z-score. Distortion: using Pyrogen dictionary to score ligand. -
Cover MRC Case Studies FAW09
https://www2.mrc-lmb.cam.ac.uk/archive/articles/Reviewing_translation_at_the_MRC.pdf15 Apr 2015: enables automation of the test and scoring and also for a suite of. ... tests to be carried out rapidly (10-20 min), which makes it attractive. -
Tutorial 5: Hypothesis Testing –Answers Sheet Rob Nicholls – ...
https://www2.mrc-lmb.cam.ac.uk/download/tutorial-5-hypothesis-testing-2/?wpdmdl=18823&refresh=667ebedeee905171958243027 Mar 2014: Task 3. 1. hist(Nile). qqnorm(Nile). 2. shapiro.test(Nile). Answer : p-value = 0.04072. ... should use the Wilcoxon signed-rank test in order to test for differencesin the median. -
MD1-100 The Angstrom Additive Screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-02.pdf23 Sep 2016: 4. The ANGSTROM Additive Screen Conditions A1-D12 MD1–100/MD1-100-FX. Cryo test Key - = no cryoprotection = cryoprotected /- = some cryoprotection. ... 5. The ANGSTROM Additive Screen. Conditions E1-H12 MD1–100/MD1-100-FX. Cryo test Key - = no -
Protocol for Hamilton LABSTAR
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Manuals/Protocol_for_LabStar.pdf11 Dec 2017: 2. Place a test plate on the motorized SBS carrier of the plate sealer. ... Test the plate sealer three times. 3. Then, run the nanoliter dispenser to set 100-nL droplets of test solution in a test plate. -
Using Coot for model building into EM maps:A tutorial ...
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/EM-Tutorial-Coot-PE.pdf8 Sep 2015: The test case for the tutorial is F420-reducing NiFe hydrogenase (Frh) {Allegretti:2014tz}. ... As a test case, we are going to use chain A from Frh. -
Impress
https://www2.mrc-lmb.cam.ac.uk/coot/ephemera/beyond-coloured-lines.pdf28 Sep 2020: 28 / 50. Atoms Clashing. Test-bed/Hello World. Ribosomes at 60FPS. Ribosomes at 60FPS. -
IL-17 is a neuromodulator of Caenorhabditis elegans sensory responses
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Nature_2017.pdf13 Jan 2017: ARTICLE RESEARCH. To test whether ILC17.1 altered RMG responsiveness to input from O2 sensors, we used YC2.60 Ca2+ sensors26 to image RMG and URX (as a control) in freely ... Test and control plates were kept in the dark until animals were picked to -
supplementary figures 070814 JL
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/scc32014si.pdf8 Aug 2014: flow through, b: bound). (B) To test if Sc Scc3 forms homodimers, full length. ... spores. (B) Mating tests demonstrating non-mating behavior of diploid and FACS. -
doi:10.1016/j.pbiomolbio.2004.07.009
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/stock2005robot.pdf19 Jan 2005: B) Aspiration of reagents from 15 ml test tubes held in a cooled rack. ... the desired chemical/buffer from 15ml test tubes held in arack on the robot platform. -
Journal of Visualized Experiments www.jove.com Copyright © 2018…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/JOVE2018.pdf19 Apr 2018: Place a test plate on the motorized SBS carrier of the plate sealer. ... Repeat this test twice more to verify that the plate sealer is ready.3. -
Protocol for Tecan EVO
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Manuals/Protocol-for-Tecan-EVO.pdf11 Dec 2017: 2. Place a test plate on the motorized SBS carrier of the plate sealer. ... Repeat this test twice more to verify that the plate sealer is ready. -
Sensory Neurons Arouse C. elegans Locomotion via Both Glutamate and…
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-4.pdf30 Jun 2015: Scale bar indicates 10 μm. p values are indicated for each comparison (Kolmogorov-Smirnov test). ... Statistical significance was determined using one-way ANOVA with Tukey test for multiple comparisons. -
Tutorial 5: Hypothesis Testing –Answers Sheet Rob Nicholls – ...
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_statistics_course_2014/tutorials/hypothesis_testing_answers.pdf27 Mar 2014: Task 3. 1. hist(Nile). qqnorm(Nile). 2. shapiro.test(Nile). Answer : p-value = 0.04072. ... should use the Wilcoxon signed-rank test in order to test for differencesin the median. -
Bacterial actin: architecture of the ParMRC plasmidDNA partitioning…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/ParMRC%20Salje%202008%20EMBO.pdf4 Aug 2008: Ent et al, 2002). To verify and test this interaction further, 14 single-point. ... et al, 2002) just below loop 2 (Figure 4F–H). To test for. -
Mechanism of ribosome stalling during translation of a poly(A) tail
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/NSMB_2019.pdf25 Nov 2019: RNCs were produced using this strategy in which one of three test sequences (7"K, 3"K and 1"K, as indicated) is positioned inside the ribosome tunnel. ... If a test sequence inserted downstream of the FLAG-SR region causes terminal stalling, production -
MD1-93 the Morpheus Additive Screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-01.pdf5 Jul 2018: Test a large range of precipitants, stabilizers, buffers, nucleants, phasing compounds, cryoprotectants and surfactants all in one screen. • -
https://www2.mrc-lmb.cam.ac.uk/microscopes4schools/downloads/protocols
https://www2.mrc-lmb.cam.ac.uk/microscopes4schools/downloads/M4S_activities.pdf11 Mar 2022: Lugol solution is a solution of iodine and potassium iodide in water and it is used as an indicator test for the presence of starches, with which it reacts by turning -
KWRM_A_992668_O
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-3.pdf20 Oct 2015: provides a critical test for functionalimportance. Indeed, optogenetic techni-ques to artificially manipulate neuronalactivity at high spatio-temporal resolutionhave been transformative for neurosci-ence.12,13 In a similar manner, techniquesto -
The MORPHEUS protein crystallization screen
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/morpheus%202009.pdf12 Nov 2009: Manydivalent cations and some carboxylic acids were discarded inlater tests because of problems with stability and false posi-tives. ... 3. Results and discussion. Both well known test proteins and novel samples were triedwith MORPHEUS. -
doi:10.1016/j.jbbm.2005.12.008
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/rfcloning2006%20published.pdf19 Apr 2006: TA. Fig. 2. Protein expression and solubility tests. A. Once transformed to BAI cells, protein expression was tested in 10 ml. ... B. Test expression of. the 136 kDa BSSMC protein at 25 8C in C41 cells (lane 2) and BAI cells (lanes 4). -
Clay_thesis
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/AnthonyKempf.pdf4 Oct 2005: receptors. To test this possibility, we performed ablations that eliminated the egg-laying. ... laying interval times, a non-parametric test was used (the Mann-Whitney rank test). -
JFCregg_OGS_finaledit
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/Cregg_thesis.pdf14 Dec 2010: eScholarship provides open access, scholarly publishingservices to the University of California and delivers a dynamicresearch platform to scholars worldwide. Electronic Theses and DissertationsUC San Diego. Peer Reviewed. Title:Pharmacological -
554 Research Paper Inhibition of receptor-mediated endocytosis by the …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/wigge_McMahon1997.pdf21 Mar 2003: To test whether the cellulartarget of amphiphysin is dynamin, COS-7 cells were cotransfected with bothdynamin and the amphiphysin SH3 domain; here, transferrin uptake wasefficiently rescued. ... To test the hypothesis that the SH3 domain ofamphiphysin is -
doi:10.1016/j.molcel.2004.08.030
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Haering%20Mol%20Cell%202004.pdf20 Sep 2004: However, the disorder makes it difficult todesign specific mutants to test this hypothesis.its C terminus (Figure 1D). ... To test whether the S525N mutation Is There Any Turnover of Cohesin Subunits. -
318-320 News Feat Curves MH.indd
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/NatureAheadCurve.pdf17 Jul 2009: the artificially high concentrations present in the test tube. ... In the late 1990s, his graduate student Lars Dreier was mixing nuclear-envelope membranes with DNA, attempting to imitate in a test tube the way that the envelope reforms after cell -
3747_0_supp_7_1052456676
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/christian.pdf9 May 2003: calcium concentration. To study the function of VGCCs we proceeded to test if. ... subunit UNC-36. Before proceeding to test VGCC mutants and subunit mutants we. -
Changes in Postural Syntax Characterize Sensory Modulation and…
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-5.pdf14 Aug 2015: A rank sum test comparingposture probabilities during and outside of stimulation periods shows that dorsal bends areover-represented (Fig 3C) during stimulation, as previously reported for this strain [21]. ... Hits are trigrams with significantly -
Identifying proteins bound to native mitotic ESC chromosomes reveals…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Djeghloul%20Nat%20Camms%202020.pdf11 Aug 2020: P-values of statistically significant increases, measured by unpaired two tailed Student’s t-tests, are indicated. ... P-values of statisticallysignificant decreases, measured by unpaired two tailed Student’s t-tests, are indicated. -
73687_Trans
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/emboj201099s2.pdf28 Apr 2010: Therefore, if F17 does not insert into the membrane bilayer (see above) the authors should test possible effects of the F17V mutation on DivIVA higher-order oligomerization by analytical ultra centrifugation. ... It should be straightforward to test this -
1 Supplementary data to accompany paper: Mechanism of endophilin ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndophilinEMBO/EndoSupMethods.pdf8 Jun 2006: simple gaussian functions to dc/dt versus s curves to test for the number of components and. ... Rfree test set size (%) 5.1 % outliers 0.5. <B> (Å2) 69. -
Tutorial 5: Hypothesis TestingRob Nicholls –…
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_statistics_course_2014/tutorials/hypothesis_testing.pdf26 Mar 2014: The F-test tests the null hypothesis that the variancesof two samples are equal using the test statistic:. ... 3 One-sample tests. One-sample t-test. The one-sample t-test tests the null hypothesis that the population mean is equalto some value µ0. -
© 2005 Nature Publishing Group Endophilin and CtBP/BARS are ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndopohilinNature2005.pdf4 Jan 2006: Thus, a differentapproach is needed to test whether endophilin or CtBP/BARS doindeed have LPAAT activity.In a previous study1, the LPAAT activity of endophilin was. ... To test the possibility that LPAAT activity is not intrinsic toendophilin, we used a -
The ER membrane protein complex promotes biogenesis of sterol-related …
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/JCS_2019.pdf18 Jan 2019: are shown (n=4). P0.01, P0.001 (Student’s t-test). 3. RESEARCH ARTICLE Journal of Cell Science (2019) 132, jcs223453. ... are shown (n=3).P0.001, P0.0001 (Student’s t-test). Reconstitution of Δ5 (D) and Δ6 cells (E) with empty vector control (EV), -
Elementary hypothesis testing • Introduction • Some distributions…
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_statistics_course_2014/lectures/Lecture5_hypotheses.pdf7 Apr 2014: multiple hypothesis var.test - test for equality of variances power.t.test - to calculate power of t-test Some other tests. ... of two random variables are same Grubbs test – tests for outliers (more in R package – outliers). -
Tutorial 5: Hypothesis TestingRob Nicholls –…
https://www2.mrc-lmb.cam.ac.uk/download/tutorial-5-hypothesis-testing/?wpdmdl=18817&refresh=667ebeded42f7171958243026 Mar 2014: The F-test tests the null hypothesis that the variancesof two samples are equal using the test statistic:. ... 3 One-sample tests. One-sample t-test. The one-sample t-test tests the null hypothesis that the population mean is equalto some value µ0. -
se060101051p
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/ford01.pdf3 Dec 2004: test-ed by sedimentation assays using liposomesor lipid tubules (19, 27, 28). ... Each assay contained0.05 mg/ml protein. (B) Liposomes containing 10%cholesterol, 40% phosphatidylethanolamine, 40%phosphatidylcholine, and 10% of a test lipid wereprepared -
The structure of human thyroglobulin
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/TG_Nature2020.pdf31 Jan 2020: Nature, doi:10.1038/s41586-020-1995-4 -
Thesis Chapter 0
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/Hardaker.pdf10 Jun 2008: To test the possible involvement of FLP-1-encoded peptides in the modulation of egg-. -
MD1-91, MD1-92 and MD1-92-FX Morpheus II 10 mL, HT-96 and FX-96…
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB20.pdf9 Jul 2018: Figure 1. Examples of crystals obtained during initial tests with the final formulation of Morpheus II. -
MD1-46, MD1-47 and MD1-47-FX Morpheus 10 mL, HT-96 and FX-96…
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB17.pdf9 Jul 2018: Preliminary tests with Morpheus made within the Laboratory of Molecular Biology (LMB)1 at Cambridge, UK, have shown encouraging results with various targets. -
httDownloaded from rsif.royalsocietypublishing.org ResearchCite this…
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-1.pdf4 Feb 2015: 2.12. Statistical analysisStandard statistical tests were performed using MATLAB func-tions. Correlation coefficients were calculated using theMATLAB function corrcoef. ... A value of þ1 indicates perfect positivecorrelation. A value of 21 indicates -
M410_8e 231..235
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Dynamin.pdf6 Mar 2001: In an effort to distinguish betweenthese two hypotheses, we designed mutants of dynamin that wouldenable us to test the relationship between dynamin's GTPase activityand its ability to support endocytosis. ... We then embedded cells asdescribed23. To -
se060101051p
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsin/EM/ford.pdf3 Dec 2004: test-ed by sedimentation assays using liposomesor lipid tubules (19, 27, 28). ... Each assay contained0.05 mg/ml protein. (B) Liposomes containing 10%cholesterol, 40% phosphatidylethanolamine, 40%phosphatidylcholine, and 10% of a test lipid wereprepared -
Tutorial 6: Linear RegressionRob Nicholls –…
https://www2.mrc-lmb.cam.ac.uk/download/tutorial-6-linear-regression/?wpdmdl=18818&refresh=667ebedf2f29f171958243131 Mar 2014: This essentially tests whether the. Tutorial 6: Linear Regression 4. model has any predictive power. ... b) Create a model to test this hypothesis, assuming a linear relationshipbetween Height and Girth. -
pgen.1004082 1..13
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/84_Chen_C_PlosGen_2014.pdf24 Sep 2015: indicatesp,0.01 by ANOVA, Bonferroni’s multiple comparisons test.(TIF). Figure S6 ODR-10-GFP localization in mutants expressingdefective rab proteins. ... p,0.01 (ANOVA, Bonferroni’s multiple comparisons test).(TIF). Figure S7 Deleting ufc-1 or uba-5 -
Supplementary Figure 1Lysophosphatidic acid acyl transferase (LPAAT)…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndoSupFigs.pdf4 Jan 2006: This software was also used for the direct fitting of simple gaussian functions to dc/dt versus s curves to test for the number of components and estimate their molecular mass.
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