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https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Reim_Brose2001.pdf17 Mar 2003: amplitudes. In contrast, EPSC amplitudes of synchronous release (Geppert et al., 1994).in double mutant cells increased during the first few To test the possibility that CPX I/II deficiency causesstimuli ... Third, maximal values of synchronoussynaptic -
A-nsmb855.indd
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nsmb%20FtsZ%202004.pdf19 Nov 2004: The fact that the domain swap is possible and a report describing two-stage unfolding kinetics of FtsZ24 prompted us to test whether the N- and C-terminal domains of FtsZ ... To test for bound nucleotide, FtsZ was precipitated using 0.5 N cold HClO4, and -
DOI: 10.1126/science.1165322 , 1710 (2008); 322Science et al.Rachel…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/19008417.pdf18 Dec 2008: To further test the invivo role of the Sulfolobus ESCRTcomponents, wegenerated an episomal vector to direct arabinose-inducibleexpressionof atargetgeneinS.solfataricus(21) (figs. -
Phil. Trans. Roy. Soc. Lond. B. 261, 95-104 (1971) ...
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ptrs71.pdf26 Nov 2012: The thin carbon film is a good phase test object (see Thon 1966) and, if the thickness of gold is correct, it displays high contrast phase detail, in which points only -
Substrate-driven assembly of a translocon for multipass membrane…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2023/08/Nature_2022b.pdf19 Aug 2023: To test whether internal loop translocation occurs at the secretory translo-con, we analysed biogenesis intermediates of EAAT1, an eight-TMD protein28 with a glycosylated luminal loop after TMD3 (Fig. ... Statistical analyses in e,g were performed by -
53091 361..366
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/McMahon01020.pdf3 Dec 2004: Liposome tubulation, sedimentation and electron microscopySynthetic liposomes contained 10% cholesterol, 40% phosphatidylethanolamine,40% phosphatidylcholine, and 10% of the test lipid (in most casesphosphatidylinositol(4,5)bisphosphate). -
15211199233993 1..45
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MreBHussain2018.pdf15 Mar 2018: thetic complex. To test this hypothesis, we examined MreB motions as we changed the shape of. ... increase with cell width (Ouzounov et al., 2016). To test if the angle of MreB movement changes. -
Expression, purifica0on, characteriza0on, and crystalliza0on So how…
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_crystallography_course_2013/lectures/20130430_Cryst_course.pdf2 May 2013: Beware of the layer of cold air over the liquid nitrogen (2) Test crystal at room temp: Mitegen crystal sleeve: MicroRT Test different cryos & precipitant concentra-ons Remove any mother liqour -
CURRICULUM VITAE
https://www2.mrc-lmb.cam.ac.uk/groups/bienz/wp-content/uploads/sites/23/BienzCV_March2024.pdf11 Apr 2024: signalling pathway in the Drosophila midgut. Development 122, 849-858. 47. Hart K & Bienz M (1996) A test for cell autonomy, based on di-cistronic messenger. -
doi:10.1016/j.molcel.2008.05.027
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsK%20MolCell%202008%20Lowe.pdf16 Aug 2008: loading of a FtsK hexamer upon interaction with KOPS. This assay provides a good test for efficiency of loading of. ... sequently activate recombination. In order to test whether the dimeric forms of the protein had. -
Mechanism of signal sequence handover from NAC to SRP on ribosomes…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2023/08/Science_2022.pdf27 Feb 2022: To test this, we compared the affinity ofNAC for RNCs displaying either an ER signalsequence (RNCSS) or a mutated signal sequencethat inhibits ER targeting (RNCSSmt) (fig. ... S17, B and C). Thus, thecontact between NAC UBA and SRP54 NG do-mains -
4380 | G. J. Doherty et al. Molecular Biology ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Doherty2011/DohertyGRAF_MBC2011.pdf17 Jan 2012: Bars and error bars correspond to mean and SEM calculated from three independent experiments (n > 250; α = 0.05; two-tailed Fisher’s exact test, , p < 0.001). ... Bars and error bars correspond to mean and SEM (n = 12–15; α = 0.05; Student’s t -
Cellular/Molecular Endophilin Drives the Fast Mode of Vesicle…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Llobet%20J%20Neurosci2011.pdf23 Sep 2011: AnANOVA test confirmed that the amplitude of the fast phase wassignificantly reduced by endophilinSH3 (p 0.01). ... jneurosci.org as supplemental material), soit was important to test the specificity of its effect. -
Substrate-specific regulation of the ribosome–translocon junction by…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/17_Rutkowski_DT_PNAS_2001.pdf22 Jun 2001: To test this idea, we directly comparedthe translocation efficiencies of pPrl containing the pPrl, pbL, orpIgG signal sequences. ... translocation efficiency of bL-Prl and IgG-Prl. To test thishypothesis, we added either the first 5, 54, or 117 aa of the -
Calcium-mediated Evidence from the Changes in Gap Junction Low ...
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb83.pdf1 Nov 2012: Acid-washed glassware was used in experiments designed to test sensitivity o f gap j u n c t i o n structure to divalent cations. -
Volume 22 May 15, 2011 1625 Cytosolic aggregates perturb ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/69_Chakrabarti_O_MBC_2011.pdf11 Nov 2011: The aggregated protein indicated at left was coexpressed with the test protein indicated along the top. ... At each time point, the % of aggregate-containing cells that showed excess intracellular accumulation of the test protein is indicated. -
pone.0107211 1..10
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/leoa2014.pdf3 Sep 2014: DLPs. We attempted to test this hypothesis directly but were. unable to obtain either individually, in combination, or expressed. -
MR_LMB
https://www2.mrc-lmb.cam.ac.uk/download/molecular-replacement/?wpdmdl=18799&refresh=668ea3bde0d8b172062406111 Jun 2013: approximate • conformational change. • Test multiple choices of model • easier in a pipeline: phenix.MRage, Balbes, MrBUMP • multiple templates,. -
Single‐dose immunisation with a multimerised SARS‐CoV‐2 receptor…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/COVID%20vaccine%202021%201873-3468.14171.pdf29 Aug 2021: outside of the sphere. We aimed to test whether Dps could efficiently dis-. ... Spike-S-Dps sera gave very little neutralisation. Whilst. pseudoviruses are widely used to test the neutralisation. -
doi:10.1128/mBio.00800-13. 4(6): .mBio. Midcell DNA Translocase FtsK…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mbio2013bisicchia.pdf23 Dec 2013: To test whether the translocase is responsiblefor promoting and maintaining the hexameric state of FtsK invivo, we determined the stoichiometry of an FtsK derivative lack-ing the C-terminal domain (FtsKC;
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