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13 Jun 2022: To test for physical interactions between these putativeESCRT-I proteins, we recombinantly expressed in E. ... To test this idea, weused size-exclusion chromatography (Fig. 3A) and SEC-MALS(Fig.

9 Sep 1998: We sought to test whether this specialproperty could be explained by a change in the multimeriz-ation state of dynamin upon amphiphysin binding. ... To test the effect of the amphiphysins ondynamin multimerization, various recombinant SH3domains (5µg

10 Oct 1997: To test this directly, Amph2 immunoprecipi-tates were Coomassie-stained, revealing that Amph1. ... This provided us with a useful tool to test theimportance of the heterodimer in vivo.

10 Oct 2014: optimise functionality; streamline feedback cycle. • Test suitability of merging up the hierarchy (when appropriate). • ... bias. Concerns Raised: • Potential for cross-validation:. – Is retaining a “test set” of molecules appropriate? –

21 Feb 2011: To test these activities, wecoexpressed full-length Rvs161 and Rvs167 using an E. ... To-gether, our biochemical tests show that Rvs161-Rvs167 bindsto membranes and induces three-dimensional curvature.

8 Jun 2006: implying the formation of additional helical structures. To test. how the predicted N-terminal amphipathic helix folds,. ... seeResults). Student’s t-test Po0.001, Po0.2. Endophilin and vesicle buddingJL Gallop et al. &

16 Feb 2010: Fortuitously, certain disease-causing prion protein (PrP) mutants meet these requirements and afford a unique opportunity to test whether in vivo signal sequences display either appreciable inef"ciency or functional differences. ... To test this idea

16 Jul 2023: S1E). As a further test of thisfinding, we expressed the Vps4E209Q plasmid in a cell line carryinga thymidine kinase homolog (hereafter called STK). ... 3A).To test the functional significance of this patterned assembly ofESCRT-III polymers, we used

7 Dec 2006: To test for dimerization invivo we turned to the bacterial two-hybrid system of Kari-mova et al. ... We thereforedecided to test whether B. subtilis MreC interacteddirectly with various PBPs.

6 Dec 2014: 1a,d). MinC and MinD copolymerize to form an alternatingcopolymer. To experimentally test filament formation, we usedfull-length, untagged E. ... To test the effect of MinE on MinCD filaments,. we added MinE to the same experiment as described in Fig.

15 Nov 2011: kDa dextran were significantly different (paired Student’s t test, P < 0.001,n = 6). ... We used two experimental approaches, transfection andmicroinjection, to test the effects of this domain on HA-initiatedsyncytium formation.

21 Sep 2006: Statistical comparisons of Deff values were performedby using two-tailed Student t tests.Statistically significant change (P 0.005) relative to the steady-state. ... Fluorescencerecovery curves were plotted by using KALEIDAGRAPH 3.5 (SynergySoftware). We

21 Jun 2005: To test for polymerization formation, the refoldedprotein was analyzed as described for electron microscopy.

6 Oct 2004: Rap and PtdIns(3,4,5)P3 Activate ARAP3 Rho GAPActivity In VivoTo test whether a Rap family member activates ARAP3’sRho GAP activity in vivo, we performed more

6 Mar 2008: 74. Table 4.5: The results of the significance test (p-value < 0.05 is considered to be significant).

1 Dec 2019: Circ Genom Precis Med. 2019;12:e002507. DOI: 10.1161/CIRCGEN.119.002507 September 2019 397. BACKGROUND: Pediatric cardiomyopathies are a clinically and genetically heterogeneous group of heart muscle disorders associated with high morbidity and

11 Aug 2008: To test this, wegenerated liposomes with low curvature (800 nm in diameter)and high curvature (50 nm in diameter) and assayed binding ofArf1–GTP and Arf6–GTP to these liposomes (see

18 Apr 2006: through an adaptor protein. To test whether these two. complexes can directly interact in vitro, we used analyt-. ... Avanti or Echelon). Control vesicles contained an additional 10% PC. instead of the test lipid.

4 Oct 2007: tion. Mutant parR genes were tested for functionality in their. natural genetic context on test plasmids derived from low-. ... As shown in Figure 4A, test plasmids. containing the ParR171 K6E, R7S and K8E mutations severely.

18 Dec 2008: To further test the invivo role of the Sulfolobus ESCRTcomponents, wegenerated an episomal vector to direct arabinose-inducibleexpressionof atargetgeneinS.solfataricus(21) (figs.