Search

8 Sep 2006: In tests for MT. binding, the Kar3 protein showed a Kd of 0.37 6 0.10 mM with no.

22 Aug 2007: sufficient overexpression to test if the network can be distorted and overexpressed.

22 Mar 2005: In contrast,EE/RE, was inhibited in CHC RNAi-treated cells. Welarge amounts of STxB/HRP were found in clathrin-con-used quantitative biochemical techniques to test thistaining early endosomal vacuoles ... To test whether AP1 is required for the retro-

17 Mar 2014: Compare means to test for differences. • Consider variance (and shape) of the distributions – help choose appropriate prior/protocol. •

2 May 2013: Beware of the layer of cold air over the liquid nitrogen (2) Test crystal at room temp: Mitegen crystal sleeve: MicroRT Test different cryos & precipitant concentra-ons Remove any mother liqour

19 Mar 2013: 3C). To further test for auto-inhibition, we per-formed a series of co-sedimentation assays with SUVs (Fig. ... 42). Although our assay does notallow us to test for possible scission mechanisms, we can ask thefollowing question.

11 Aug 2015: To test this directly, we used nondenaturing affinity purification to assess a possible 60S-listerin-translocon ternary complex (Figure 1E). ... To test this, we treated cultured cells with either the transla-tion elongation inhibitor cycloheximide (CHX)

8 Dec 2007: To test whether MIMs have a role in sorting, we mutated motifs inboth Vps2 and Did2.

17 Feb 2010: B) MreB polymerisation assays wereused to test the interface mutants of RodZ for MreB binding.

29 Aug 2012: The crystal structure of the complex between BETA and BLIP has been a test case for molecular replacement because of the difficulty encountered in the original structure solution.

16 Oct 2009: Future geneticstudies will allow us to test this possibility. References and Notes1.

4 Apr 2003: The best estimate was taken to be the defocus yielding the highest image Quality, Qi, de- fined as the number of test phases in agreement with (Iphase difference1 < 90’) the centrosymmetric ... 13). To test how sensitively PQ measures the signal-to-

13 Jun 2009: an in vivo interaction assay based on coassociation of an. FP-tagged test protein with FP-tagged cytosolic PrP aggregates. ... involving excessive PrP exposure to the cytosol. To test this. idea, we analyzed the status of Mgrn and lysosomes in trans-.

30 Jun 2004: parS. sequence5. ¢. -TGTTCCACGTGAAACA-3. ¢. (Fig. 1A and B). To test forthe ability of wild-type and truncated proteins to carry outDNA binding and spreading, we used an agarose gel

15 Jul 2005: 2e). To test whether this difference underlies cotransin’ssubstrate selectivity, we prepared chimaeric constructs in which thesignals of VCAM1 and pPrl were fused to an otherwise non-translocated reporter. ... 3a), these results suggestthat cotransin

18 Dec 2022: To test thesehypotheses, we used the same N-terminal Brn1 release assay(Fig.

13 Jun 2009: It will then be possible to test in transgenicmice whether the effects of the HD mutant stem solely fromCtmPrP production or through other mechanisms (e.g. ... PrP gener-ation, trafficking and degradation so that they can bemanipulated selectively to

12 Oct 2012: Imageswere obtained with a cooled EMCCD camera (9100-02; Hamamatsu). Statistics. Significance was tested using the 2-tailed Student t test. ... To test the impact of PSTPIP2-deficiency disease in mice onosteolytic responses in vivo, we performed

4 Oct 2010: Thus, our first goalwas to test whether the overall structure of the endophilin BARdomain is retained upon membrane interaction and, if so, howits concave surface interacts with the membrane. ... To test for potential movement between thecentral region

4 Mar 2008: Expression tests were done inBl21AI cells (Invitrogen) and C41 cells (Miroux and Walker,1996) with a 10 ml culture and purified over a Ni2 NTA spincolumn as described previously (van den