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29 Aug 2021: outside of the sphere. We aimed to test whether Dps could efficiently dis-. ... Spike-S-Dps sera gave very little neutralisation. Whilst. pseudoviruses are widely used to test the neutralisation.

11 Jun 2013: approximate • conformational change. • Test multiple choices of model • easier in a pipeline: phenix.MRage, Balbes, MrBUMP • multiple templates,.

19 Apr 2002: To test whether Smc3 s head alone is sufficient. to bind Scc1, we created an artificial head in which25 min, whereas the on rate of Smc3 was about twicethat of its ... To test this directly, we repeated the aboveexperiment using a yeast strain expressing

15 Apr 2004: Each bar represents the mean SD (n 10), with statistically significant differences (P 0.01 using t test) being observed between the two conditions. ... multiple days.aTreatment is significant (P 0.01) compared with untreated cells using t test.

21 Nov 2014: S7E). Sister DNAs are entrapped bycohesin trimers. To test whether cross-linking all three interfacesof the cohesin ring entraps sister DNAs in a co-valent topological embrace, we isolated dimersof a

17 Jun 2005: To test if a peptide de-rived from rat amphiphysin would inhibit the amphi-physin-AP2 interaction in goldfish, we performed abinding experiment using a GST-tagged construct of ratamphiphysin 1 ... ease of vesicles that were not primed at the time

17 Mar 2003: test set of reflections, consisting of a randomly selected 5% of the data and not used during refinement.

5 Dec 2012: To test the biological relevance of Gbg-mediated activation in p110bsignaling, we compared the ability of wild-type and mutant p110bconstructs to mediate cellular transformation and motility. ... To test the role of p110b-Gbginteractions in PTEN-null

2 May 2013: Beware of the layer of cold air over the liquid nitrogen (2) Test crystal at room temp: Mitegen crystal sleeve: MicroRT Test different cryos & precipitant concentra-ons Remove any mother liqour

26 Jan 2006: 1E)(Ford et al., 2002). As many other proteins are predicted to containN‐terminal amphipathic helices, the liposome tubulation assay will provea valuable test for the folding and insertion of these

8 Sep 2006: In tests for MT. binding, the Kar3 protein showed a Kd of 0.37 6 0.10 mM with no.

6 Apr 2017: The model was improved manually using MAIN(ref. 38) and refined with PHENIX and REFMAC (refs 39, 40), keeping the 5%randomly selected test set of reflections separate at all times.

17 Mar 2014: Compare means to test for differences. • Consider variance (and shape) of the distributions – help choose appropriate prior/protocol. •

22 Aug 2007: sufficient overexpression to test if the network can be distorted and overexpressed.

22 Mar 2005: In contrast,EE/RE, was inhibited in CHC RNAi-treated cells. Welarge amounts of STxB/HRP were found in clathrin-con-used quantitative biochemical techniques to test thistaining early endosomal vacuoles ... To test whether AP1 is required for the retro-

19 Mar 2013: 3C). To further test for auto-inhibition, we per-formed a series of co-sedimentation assays with SUVs (Fig. ... 42). Although our assay does notallow us to test for possible scission mechanisms, we can ask thefollowing question.

29 Aug 2012: The crystal structure of the complex between BETA and BLIP has been a test case for molecular replacement because of the difficulty encountered in the original structure solution.

8 Dec 2007: To test whether MIMs have a role in sorting, we mutated motifs inboth Vps2 and Did2.

11 Aug 2015: To test this directly, we used nondenaturing affinity purification to assess a possible 60S-listerin-translocon ternary complex (Figure 1E). ... To test this, we treated cultured cells with either the transla-tion elongation inhibitor cycloheximide (CHX)

17 Feb 2010: B) MreB polymerisation assays wereused to test the interface mutants of RodZ for MreB binding.