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80096 RPF
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/EMBO2012FtsAPiotr/emboj201276s3.pdf7 Mar 2012: The reason for this is probably that the C-terminal amphipathic helix needs to be in contact with the membrane for polymerisation to occur at test-tube concentrations and in the ... Obviously, there are limitations as to what is possible, determined by -
088542u047
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Owen_McMahon1999.pdf17 Mar 2003: In order to test the validity of this hypoth-by six residues to complete the b strand, resulted in a. -
Chapter 20 In Vitro Dissection of Protein Translocationinto the ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/62_Sharma_AS_MMB_2010.pdf30 Apr 2010: 2. Layer this onto 100 µL of the sucrose cushion in an ultra-centrifuge tube (either a tube for the TLA120.1 rotor or amicro-test tube for the TL100.3 ... 1. Using glass measuring tools (e.g., glass pipettes), transfer20 mg PC and 5 mg PE to a glass -
The Jour nal o f Cel l Bio logy ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/24_Fons_RD_JCB_2003.pdf6 Feb 2003: To test whether the tetrameric TRAP complex alone wasthe component that stimulates. ... To test this, we analyzed theTRAP dependence of two point mutations in the PrP signalsequence (termed N7 and N9 [Fig. -
Protein-driven membrane stresses in fusion and fission
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/KozlovCurvature2010.pdf21 Feb 2011: Science 321, 1354–1357. 62 Sever, S. et al. (2000) Garrotes, springs, ratchets, and whips: puttingdynamin models to the test. -
refmac keywords
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/refmac/refmac_keywords.pdf12 Jan 2012: V3000 has not beentested yet. If somebody wants to test please let me know. -
The Jour nal o f Cel l Bio logy ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/26_Snapp_EL_JCB_2003.pdf16 Oct 2003: To test this, we fused GFP or YFP to thecytoplasmic domains of three resident ER membrane proteinswith minimal cytoplasmic domains: two rough ER com-ponents of the translocon, Sec61. ... chimera. To test if OSER-inducing, GFP-tagged ER proteinswere -
Tutorial 2: Descriptive Statisticsand Exploratory Data Analysis…
https://www2.mrc-lmb.cam.ac.uk/download/tutorial-2-descriptive-statistics-2/?wpdmdl=18820&refresh=66a660b5df9d5172217976520 Mar 2014: Petal.Width and systematically higher Sepal.Width thanthe other two species, although to draw such conclusions we would have to test suchhypotheses properly). -
Qrb1300006 1..40
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/qrb13.pdf30 Jul 2013: twist around the tube axis). Preliminary tests using a segment size similar to that in Fig. -
SVUS002-16.tex
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/31_Rane_Chapter_2005.pdf21 Sep 2006: Meanwhile, the most definitive test for prions, a bioassay, was be-ing performed in parallel on comparable samples3639. ... These toolscan then be used in vivo to test specific hypotheses regarding the patho-genesis of prion diseases. -
Asymmetric distribution of pause transfer sequences in apolipoprotein …
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/6_Kivlen_MH_JLR_1997.pdf30 May 2008: Experiments must now be designed to test this hypothesis. Translocational pausing has been viewed as a mecha- nism by which particular regions of the nascent chain can be isolated from the -
Membrane transport between compartments in eukary-otic cells requires …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Dynaminreview2004.pdf2 Feb 2004: 4) have been used asassembly tests for other members of the dynamin family(see Oligomerization web site in Online links). ... This clathrin-centricmodel for endocytosis does not, however, take intoaccount the role of dynamins in -
THE JOURNAL OF BIOLOGICAL CHEMISTRY 0 1990 by The ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/1_Pewitt_EB_JBC_1990a.pdf29 Dec 2004: In order to test whether stimulated [3H]bumetanide binding would be re- tained in membranes after activation of transport by agents other than catecholamines, we examined specific binding in membranes from -
Tumour prevention by a single antibody domaintargeting the…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17568777.pdf22 Jul 2007: In addition,. using mouse models as a preclinical test, as we have done in. -
doi:10.1016/j.jmb.2004.12.031
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jmb05.pdf24 Jan 2005: The model-derivedphases provided a more sensitive test of the signalretained in the images at high resolution than theprevious reference, which had been derived solelyfrom the images. -
Cell, Vol. 91, 575–582, November 28, 1997, Copyright 1997 ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/9_Hegde_RS_Cell_1997.pdf20 Nov 1997: Although the signifi-. cance of these findings remains to be determined, the Indeed, the contradicting conclusions resulting frommany of the studies which each used different test sub-rearrangements of the -
The Structure of a Tunicate C-type Lectin from Polyandrocarpa…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/10398588.pdf7 Jul 1999: The residuals betweenthe tted absorbance and the experimental values wereplotted, to test for the validity of the model used. -
se060101051p
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/AP180/Ap180_st.pdf3 Dec 2004: test-ed by sedimentation assays using liposomesor lipid tubules (19, 27, 28). ... Each assay contained0.05 mg/ml protein. (B) Liposomes containing 10%cholesterol, 40% phosphatidylethanolamine, 40%phosphatidylcholine, and 10% of a test lipid wereprepared -
Regulation of lipid binding underlies the activation mechanism of…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/22120714.pdf21 Nov 2012: Oncogene. Correlation of lipid kinase activity with lipid bindingTo test whether lipid binding forms the basis of p110activation, we compared lipid kinase with lipid-bindingactivities for three sets of p110a/p85a -
Tutorial 2: Descriptive Statisticsand Exploratory Data Analysis…
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_statistics_course_2014/tutorials/descriptive_statistics_and_exploratory_data_analysis_answers.pdf20 Mar 2014: Petal.Width and systematically higher Sepal.Width thanthe other two species, although to draw such conclusions we would have to test suchhypotheses properly). -
Reconstitution of a prokaryotic minus end-trackingsystem using TubRC…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2015-Fink-E1845-50.pdf27 Apr 2015: To test for seeding, we followed filament formation onimmobilized TubRC complexes (Fig. -
Tutorial 6: Linear RegressionRob Nicholls –…
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_statistics_course_2014/tutorials/linear_regression.pdf31 Mar 2014: This essentially tests whether the. Tutorial 6: Linear Regression 4. model has any predictive power. ... b) Create a model to test this hypothesis, assuming a linear relationshipbetween Height and Girth. -
BAR Domains as Sensors ofMembrane Curvature: TheAmphiphysin BAR…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/PeterBARdomains.pdf30 Jan 2004: T-tubule net-work (7 ). To test if this tubulating ability is dueto the Drosophila amphiphysin BAR domain,we mutated pairs of basic residues to gluta-mates, Lys161 Lys163 on the ... S3B). As a functional test of the amphiphysin BARdomain, we tested the -
pone.0052401 1..15
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Podosome2012/journal.pone.0052401.pdf10 Jan 2013: Test set size (%) 5.4. Rwork (%) 19.6(22.9). Rfree (%) 25.2(23.8). ... Significance was calculated using the Student’s t test( = p,0.05). D: Confocal micrographs of RAM cells over-expressing Bin2-EGFP (Bin2, green, left) or control (EGFP, green, right). -
Combinatorial Control of Prion Protein Biogenesis by the…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/18_Kim_SJ_JBC_2001.pdf26 Jun 2001: The present work has generated a wide arrayof tools to test this and other hypotheses in future studies. -
Wild card macros
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/sciencegayathri2012/Gayathri.SM.pdf23 Oct 2012: parenthesis. † Rmerge = ΣΣ | Ihl - 〈Ih〉 | / ΣΣ 〈Ih〉 h l h l ‡ 5 % of the reflections were included in the test set. -
The GTPase-Activating Protein GRAF1 Regulates the CLIC/GEEC Endocytic …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Lundmark_McMahon_GRAF2008.pdf28 Nov 2008: The error bars show 95% confidence intervals (calculated by t tests) for each condition. -
REPORT◥ PROTEOSTASIS UBE2O is a quality control factor fororphans ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017b.pdf4 Aug 2017: Fig. 2. Reconstitution ofUBE2O-mediated clientubiquitination with purifiedfactors. (A) Experimentalstrategy to test UBE2O inter-action and ubiquitination in adefined system. -
Statistical Theory; Why is the Gaussian Distribution so popular? ...
https://www2.mrc-lmb.cam.ac.uk/download/statistical-theory-why-is-the-gaussian-distribution-so-popular-rob-nicholls/?wpdmdl=18809&refresh=66a660b658863172217976617 Mar 2014: Compare means to test for differences. • Consider variance (and shape) of the distributions – help choose appropriate prior/protocol. • -
The structural basis of novel endosome anchoringactivity of KIF16B ...
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17641687.pdf23 Jul 2007: To further test this notion, we performed two independent. measurements. First, we monitored by SPR analysis the. -
Modeling membrane shaping by proteins: Focus on EHD2 and N-BAR domains
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/KozlovEHDs.pdf7 Apr 2010: FEBS Letters 584 (2010) 1830–1839. j o u r n a l h o m e p a g e : w w w. F E B S L e t t e r s. o r g. Review. Modeling membrane shaping by proteins: Focus on EHD2 and N-BAR domains. Felix Campelo a,b, Gur Fabrikant a, Harvey T. McMahon c, -
Phosphoinositide-Specific Phospholipase Cδ1 Activity toward Micellar…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9287165.pdf20 Apr 2002: of theδ1 enzyme show similar structural features, it was of interestto test the mammalianδ1 enzyme for activation by organicsolvent. -
Molecular Biology of the CellVol. 19, 5093–5103, December 2008 ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/LynchMBC5093.pdf24 Jan 2009: significantly (p 0.002)from wild-type and Syt-1RK by a general linear model analysis of variance using the Tukey post hoc test of values. -
BAR Domains as Sensors ofMembrane Curvature: TheAmphiphysin BAR…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/PeterBARdomains.pdf?ijkey=TikQO8xVkpUak&keytype=ref&siteid=sci30 Jan 2004: T-tubule net-work (7 ). To test if this tubulating ability is dueto the Drosophila amphiphysin BAR domain,we mutated pairs of basic residues to gluta-mates, Lys161 Lys163 on the ... S3B). As a functional test of the amphiphysin BARdomain, we tested the -
CAN-11-2008 3642..3651
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/79_Basseville_A_CanRes_2012.pdf24 Sep 2015: Statistical analysisThe Student t test was carried out for each experiment. -
Protection from cytosolic prion protein toxicityby modulation of…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/30_Rane_N_EMBO_2004.pdf8 Nov 2004: To test this possibility, we analyzed the behavior of PrP. constructs containing mutations in the transmembrane do-. -
A positively charged channel within the Smc1/Smc3 hinge required for…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embosmcjingekurze2010.pdf1 Dec 2010: generate sister chromatid cohesion. To test the effect of charge neutralization on cohesin complex. -
Relocalization of STIM1 for Activation of Store-operated Ca2!Entry Is …
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/47_Ong_HL_JBC_2007.pdf24 Sep 2015: previous report (15). We wanted to test whether a model withtwo ER compartments that are functionally distinct but slowlyequilibrate with each through diffusion of Ca2! -
FtsA forms actin-like protofilaments
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/EMBO2012FtsAPiotr/emboj201276a.pdf27 Mar 2012: be the most reliable test for FtsA polymerization, as it shows. -
Structural Mapping of the Catalytic Mechanism for a…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9048554.pdf20 Apr 2002: Data statistics are listed in Table1. Test data for the calculation of freeR factors of all thesedata sets were chosen to be consistent with the selection oftest data for the native -
Supplement_Final
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/81_Shao_S_Mol_Cell_2013.pdf16 May 2013: To test this idea, we produced RNCs ina ubiquitination-deficient lysate (Hessa et al., 2011) depleted ofthe requisite E2s and ubiquitin (Figure 4C). ... This suggests that their combinationprovides the specificity for Listerin recruitment. To test -
Cryo‐EM structure of the MinCD copolymeric filament from Pseudomonas…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Szewczak-Harris_et_al-2019-FEBS_Letters.pdf22 Jul 2019: by MinD’s MTS. To test whether P. aeruginosa MinC and MinD pro-. -
Membrane Protein Insertion at the Endoplasmic Reticulum
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/75_Shao_S_Ann_Rev_2011.pdf9 Nov 2011: The apparentfree-energy contribution of each amino acidin any test sequence was found to be additive,with the sum determining the final insertionprobability. ... earlier transmembrane domains ( gray), and a later test sequence(red ) is measured for -
Science Journals — AAAS
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2023/08/Science_2021.pdf26 Aug 2021: p values for a two-tailedStudent’s t test comparison to Q1are indicated. -
554 Research Paper Inhibition of receptor-mediated endocytosis by the …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/wigge_McMahon1997.pdf?cmd=Retrieve&db=PubMed&list_uids=9348539&dopt=Abstract21 Mar 2003: To test whether the cellulartarget of amphiphysin is dynamin, COS-7 cells were cotransfected with bothdynamin and the amphiphysin SH3 domain; here, transferrin uptake wasefficiently rescued. ... To test the hypothesis that the SH3 domain ofamphiphysin is -
Twinning and other pathologies Andrey Lebedev CCP4 OD-‐structures…
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/%22https:/www2.mrc-lmb.cam.ac.uk/download/twinningaandaotherapathologiesa-andreyalebedeva-a/?wpdmdl=18788&11 Jun 2013: Twinning tests summary. Space group validaAon. 04/06/2013 58 Crystallography Course LMB. ... Twinning tests summary. Space group validaAon. 04/06/2013 61 Crystallography Course LMB. -
PHAGOCYTES, GRANULOCYTES, AND MYELOPOIESIS PSTPIP2 deficiency in mice …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Blood-2012-Chitu-3126-35.pdf12 Oct 2012: Imageswere obtained with a cooled EMCCD camera (9100-02; Hamamatsu). Statistics. Significance was tested using the 2-tailed Student t test. ... To test the impact of PSTPIP2-deficiency disease in mice onosteolytic responses in vivo, we performed -
Roles of Amphipathic Helices and the Bin/Amphiphysin/Rvs(BAR) Domain…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Jao_JBC2010.pdf4 Oct 2010: Thus, our first goalwas to test whether the overall structure of the endophilin BARdomain is retained upon membrane interaction and, if so, howits concave surface interacts with the membrane. ... To test for potential movement between thecentral region -
Expression, purifica0on, characteriza0on, and crystalliza0on So how…
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/%22https:/www2.mrc-lmb.cam.ac.uk/download/03-protein-expression-crystallization-and-mutagenesis-meindert-lamers/?wpdmdl=18793&2 May 2013: Beware of the layer of cold air over the liquid nitrogen (2) Test crystal at room temp: Mitegen crystal sleeve: MicroRT Test different cryos & precipitant concentra-ons Remove any mother liqour -
doi:10.1016/j.devcel.2004.09.003
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/15469844.pdf1 Nov 2004: An analogous salt link is formed betweenattractive proposal. To test this, we made an N-terminalArg83 of Vps25 and Asp548 of Vps36.
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