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MR_LMB
https://www2.mrc-lmb.cam.ac.uk/download/molecular-replacement/?wpdmdl=18799&refresh=668ea3bde0d8b172062406111 Jun 2013: approximate • conformational change. • Test multiple choices of model • easier in a pipeline: phenix.MRage, Balbes, MrBUMP • multiple templates,. -
Mechanism of endophilin N-BAR domain-mediated membrane curvature…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndophilinEMBO/GallopEndophilin2006.pdf8 Jun 2006: implying the formation of additional helical structures. To test. how the predicted N-terminal amphipathic helix folds,. ... seeResults). Student’s t-test Po0.001, Po0.2. Endophilin and vesicle buddingJL Gallop et al. & -
bic054a
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/LundmarkBJ2008.pdf11 Aug 2008: To test this, wegenerated liposomes with low curvature (800 nm in diameter)and high curvature (50 nm in diameter) and assayed binding ofArf1–GTP and Arf6–GTP to these liposomes (see -
DOI: 10.1126/science.1165322 , 1710 (2008); 322Science et al.Rachel…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/19008417.pdf18 Dec 2008: To further test the invivo role of the Sulfolobus ESCRTcomponents, wegenerated an episomal vector to direct arabinose-inducibleexpressionof atargetgeneinS.solfataricus(21) (figs. -
Biallelic Variants in ASNA1, Encoding a Cytosolic Targeting Factor of …
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/CircGen_2019.pdf1 Dec 2019: Circ Genom Precis Med. 2019;12:e002507. DOI: 10.1161/CIRCGEN.119.002507 September 2019 397. BACKGROUND: Pediatric cardiomyopathies are a clinically and genetically heterogeneous group of heart muscle disorders associated with high morbidity and -
53091 361..366
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/McMahon01020.pdf3 Dec 2004: Liposome tubulation, sedimentation and electron microscopySynthetic liposomes contained 10% cholesterol, 40% phosphatidylethanolamine,40% phosphatidylcholine, and 10% of the test lipid (in most casesphosphatidylinositol(4,5)bisphosphate). -
15211199233993 1..45
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MreBHussain2018.pdf15 Mar 2018: thetic complex. To test this hypothesis, we examined MreB motions as we changed the shape of. ... increase with cell width (Ouzounov et al., 2016). To test if the angle of MreB movement changes. -
Biochem. J. (2011) 440, 185–193 (Printed in Great Britain) ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/ChernomordikBJ2011.pdf15 Nov 2011: kDa dextran were significantly different (paired Student’s t test, P < 0.001,n = 6). ... We used two experimental approaches, transfection andmicroinjection, to test the effects of this domain on HA-initiatedsyncytium formation. -
A-nsmb855.indd
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nsmb%20FtsZ%202004.pdf19 Nov 2004: The fact that the domain swap is possible and a report describing two-stage unfolding kinetics of FtsZ24 prompted us to test whether the N- and C-terminal domains of FtsZ ... To test for bound nucleotide, FtsZ was precipitated using 0.5 N cold HClO4, and -
Monitoring chaperone engagement of substratesin the endoplasmic…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/39_Snapp_EL_PNAS_2006.pdf21 Sep 2006: Statistical comparisons of Deff values were performedby using two-tailed Student t tests.Statistically significant change (P 0.005) relative to the steady-state. ... Fluorescencerecovery curves were plotted by using KALEIDAGRAPH 3.5 (SynergySoftware). We -
doi:10.1016/j.cell.2006.01.047
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/16615893.pdf18 Apr 2006: through an adaptor protein. To test whether these two. complexes can directly interact in vitro, we used analyt-. ... Avanti or Echelon). Control vesicles contained an additional 10% PC. instead of the test lipid. -
CURRICULUM VITAE
https://www2.mrc-lmb.cam.ac.uk/groups/bienz/wp-content/uploads/sites/23/BienzCV_March2024.pdf11 Apr 2024: signalling pathway in the Drosophila midgut. Development 122, 849-858. 47. Hart K & Bienz M (1996) A test for cell autonomy, based on di-cistronic messenger. -
x15959u064
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Reim_Brose2001.pdf17 Mar 2003: amplitudes. In contrast, EPSC amplitudes of synchronous release (Geppert et al., 1994).in double mutant cells increased during the first few To test the possibility that CPX I/II deficiency causesstimuli ... Third, maximal values of synchronoussynaptic -
MR_LMB
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_crystallography_course_2013/lectures/MR_LMB.pdf11 Jun 2013: approximate • conformational change. • Test multiple choices of model • easier in a pipeline: phenix.MRage, Balbes, MrBUMP • multiple templates,. -
Doc2b Is a High-Affinity Ca2+ Sensor forSpontaneous Neurotransmitter…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/GroffenScience2010.pdf30 Apr 2010: To test the influence of EGTA and BAPTA on spontaneous release, spontaneous events were recorded first in naïve cells during 1 min, followed by a EPSC recording after stimulation. ... The spontaneous release frequencies before and after loading of a Ca2+ -
Structural analysis of the ParR/parC plasmidpartition complex Jakob…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MollerJensen_ParR_EMMBO_2007.pdf4 Oct 2007: tion. Mutant parR genes were tested for functionality in their. natural genetic context on test plasmids derived from low-. ... As shown in Figure 4A, test plasmids. containing the ParR171 K6E, R7S and K8E mutations severely. -
Substrate-driven assembly of a translocon for multipass membrane…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2023/08/Nature_2022b.pdf19 Aug 2023: To test whether internal loop translocation occurs at the secretory translo-con, we analysed biogenesis intermediates of EAAT1, an eight-TMD protein28 with a glycosylated luminal loop after TMD3 (Fig. ... Statistical analyses in e,g were performed by -
pone.0107211 1..10
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/leoa2014.pdf3 Sep 2014: DLPs. We attempted to test this hypothesis directly but were. unable to obtain either individually, in combination, or expressed. -
Calcium-mediated Evidence from the Changes in Gap Junction Low ...
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb83.pdf1 Nov 2012: Acid-washed glassware was used in experiments designed to test sensitivity o f gap j u n c t i o n structure to divalent cations. -
doi:10.1016/j.molcel.2008.05.027
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsK%20MolCell%202008%20Lowe.pdf16 Aug 2008: loading of a FtsK hexamer upon interaction with KOPS. This assay provides a good test for efficiency of loading of. ... sequently activate recombination. In order to test whether the dimeric forms of the protein had.
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