Search

26 Jan 2006: 1E)(Ford et al., 2002). As many other proteins are predicted to containN‐terminal amphipathic helices, the liposome tubulation assay will provea valuable test for the folding and insertion of these

6 Apr 2017: The model was improved manually using MAIN(ref. 38) and refined with PHENIX and REFMAC (refs 39, 40), keeping the 5%randomly selected test set of reflections separate at all times.

8 Sep 2006: In tests for MT. binding, the Kar3 protein showed a Kd of 0.37 6 0.10 mM with no.

17 Mar 2014: Compare means to test for differences. • Consider variance (and shape) of the distributions – help choose appropriate prior/protocol. •

22 Aug 2007: sufficient overexpression to test if the network can be distorted and overexpressed.

22 Mar 2005: In contrast,EE/RE, was inhibited in CHC RNAi-treated cells. Welarge amounts of STxB/HRP were found in clathrin-con-used quantitative biochemical techniques to test thistaining early endosomal vacuoles ... To test whether AP1 is required for the retro-

19 Mar 2013: 3C). To further test for auto-inhibition, we per-formed a series of co-sedimentation assays with SUVs (Fig. ... 42). Although our assay does notallow us to test for possible scission mechanisms, we can ask thefollowing question.

29 Aug 2012: The crystal structure of the complex between BETA and BLIP has been a test case for molecular replacement because of the difficulty encountered in the original structure solution.

8 Dec 2007: To test whether MIMs have a role in sorting, we mutated motifs inboth Vps2 and Did2.

11 Aug 2015: To test this directly, we used nondenaturing affinity purification to assess a possible 60S-listerin-translocon ternary complex (Figure 1E). ... To test this, we treated cultured cells with either the transla-tion elongation inhibitor cycloheximide (CHX)

17 Feb 2010: B) MreB polymerisation assays wereused to test the interface mutants of RodZ for MreB binding.

16 Oct 2009: Future geneticstudies will allow us to test this possibility. References and Notes1.

4 Apr 2003: The best estimate was taken to be the defocus yielding the highest image Quality, Qi, de- fined as the number of test phases in agreement with (Iphase difference1 < 90’) the centrosymmetric ... 13). To test how sensitively PQ measures the signal-to-

30 Jun 2004: parS. sequence5. ¢. -TGTTCCACGTGAAACA-3. ¢. (Fig. 1A and B). To test forthe ability of wild-type and truncated proteins to carry outDNA binding and spreading, we used an agarose gel

13 Jun 2009: an in vivo interaction assay based on coassociation of an. FP-tagged test protein with FP-tagged cytosolic PrP aggregates. ... involving excessive PrP exposure to the cytosol. To test this. idea, we analyzed the status of Mgrn and lysosomes in trans-.

18 Dec 2022: To test thesehypotheses, we used the same N-terminal Brn1 release assay(Fig.

15 Jul 2005: 2e). To test whether this difference underlies cotransin’ssubstrate selectivity, we prepared chimaeric constructs in which thesignals of VCAM1 and pPrl were fused to an otherwise non-translocated reporter. ... 3a), these results suggestthat cotransin

4 Mar 2008: Expression tests were done inBl21AI cells (Invitrogen) and C41 cells (Miroux and Walker,1996) with a 10 ml culture and purified over a Ni2 NTA spincolumn as described previously (van den

13 Dec 2008: 5). To test the sensitivity of the model. predictions to this assumption, we repeated the calculations.

13 Jun 2009: 2.4. Northern blotting. RNA was extracted from cells using RNA STAT-60 (Tel-Test Inc.,Friendswood, TX). ... 2A). To test the functionality of the R383A and R383G mutants, flowcytometry was performed after incubating cells with the ABCG2-substrates