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https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/elife_2019.pdf29 May 2019: To test whether the role of TMED10 in GFP-PrP degradation depends on complex formation, we. -
Asgard archaea shed light on the evolutionary origins of the…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Hatano%20Nat%20Comms%202022.pdf13 Jun 2022: To test for physical interactions between these putativeESCRT-I proteins, we recombinantly expressed in E. ... To test this idea, weused size-exclusion chromatography (Fig. 3A) and SEC-MALS(Fig. -
The EMBO Journal Vol.17 No.18 pp.5273–5285, 1998 Crystal structure ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Owen_McMahon1998.pdf9 Sep 1998: We sought to test whether this specialproperty could be explained by a change in the multimeriz-ation state of dynamin upon amphiphysin binding. ... To test the effect of the amphiphysins ondynamin multimerization, various recombinant SH3domains (5µg -
Molecular Biology of the CellVol. 8, 2003–2015, October 1997 ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/WiggeKohler_McMahon1997.pdf10 Oct 1997: To test this directly, Amph2 immunoprecipi-tates were Coomassie-stained, revealing that Amph1. ... This provided us with a useful tool to test theimportance of the heterodimer in vivo. -
Systematic and Statistical Analysis to Aid Improvement and Validation …
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/2014_Oct_LIGANDS.pdf10 Oct 2014: optimise functionality; streamline feedback cycle. • Test suitability of merging up the hierarchy (when appropriate). • ... bias. Concerns Raised: • Potential for cross-validation:. – Is retaining a “test set” of molecules appropriate? – -
Molecular Biology of the CellVol. 21, 3054 –3069, September ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/RVS161_MBC2010.pdf21 Feb 2011: To test these activities, wecoexpressed full-length Rvs161 and Rvs167 using an E. ... To-gether, our biochemical tests show that Rvs161-Rvs167 bindsto membranes and induces three-dimensional curvature. -
Mechanism of endophilin N-BAR domain-mediated membrane curvature…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndophilinEMBO/GallopEndophilin2006.pdf8 Jun 2006: implying the formation of additional helical structures. To test. how the predicted N-terminal amphipathic helix folds,. ... seeResults). Student’s t-test Po0.001, Po0.2. Endophilin and vesicle buddingJL Gallop et al. & -
JCB: Article The Rockefeller University Press $30.00J. Cell…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/63_Rane_NS_JCB_2010.pdf16 Feb 2010: Fortuitously, certain disease-causing prion protein (PrP) mutants meet these requirements and afford a unique opportunity to test whether in vivo signal sequences display either appreciable inef"ciency or functional differences. ... To test this idea -
The patterned assembly and stepwise Vps4-mediated disassembly of…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Hurtig%202023%20sciadv.ade5224.pdf16 Jul 2023: S1E). As a further test of thisfinding, we expressed the Vps4E209Q plasmid in a cell line carryinga thymidine kinase homolog (hereafter called STK). ... 3A).To test the functional significance of this patterned assembly ofESCRT-III polymers, we used -
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https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MreC%20MM%202006.pdf7 Dec 2006: To test for dimerization invivo we turned to the bacterial two-hybrid system of Kari-mova et al. ... We thereforedecided to test whether B. subtilis MreC interacteddirectly with various PBPs.
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