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17 Feb 2010: B) MreB polymerisation assays wereused to test the interface mutants of RodZ for MreB binding.

16 Oct 2009: Future geneticstudies will allow us to test this possibility. References and Notes1.

4 Apr 2003: The best estimate was taken to be the defocus yielding the highest image Quality, Qi, de- fined as the number of test phases in agreement with (Iphase difference1 < 90’) the centrosymmetric ... 13). To test how sensitively PQ measures the signal-to-

30 Jun 2004: parS. sequence5. ¢. -TGTTCCACGTGAAACA-3. ¢. (Fig. 1A and B). To test forthe ability of wild-type and truncated proteins to carry outDNA binding and spreading, we used an agarose gel

13 Jun 2009: an in vivo interaction assay based on coassociation of an. FP-tagged test protein with FP-tagged cytosolic PrP aggregates. ... involving excessive PrP exposure to the cytosol. To test this. idea, we analyzed the status of Mgrn and lysosomes in trans-.

18 Dec 2022: To test thesehypotheses, we used the same N-terminal Brn1 release assay(Fig.

15 Jul 2005: 2e). To test whether this difference underlies cotransin’ssubstrate selectivity, we prepared chimaeric constructs in which thesignals of VCAM1 and pPrl were fused to an otherwise non-translocated reporter. ... 3a), these results suggestthat cotransin

4 Mar 2008: Expression tests were done inBl21AI cells (Invitrogen) and C41 cells (Miroux and Walker,1996) with a 10 ml culture and purified over a Ni2 NTA spincolumn as described previously (van den

13 Dec 2008: 5). To test the sensitivity of the model. predictions to this assumption, we repeated the calculations.

13 Jun 2009: 2.4. Northern blotting. RNA was extracted from cells using RNA STAT-60 (Tel-Test Inc.,Friendswood, TX). ... 2A). To test the functionality of the R383A and R383G mutants, flowcytometry was performed after incubating cells with the ABCG2-substrates

3 Sep 2008: some uncertainty of the molecular interpretation of the filaments,. so we carried out site-specific mutagenesis to test the contacts.

23 Jan 2012: Three-dimensional Structure of the Acetylcholine Receptor by Cryoelectron Microscopy and Helical Image Reconstruction C h i k a s h i T o y o s h i m a a n d N i g e l U n w i n. Medical Research Council Laboratory of Molecular Biology, Cambridge

9 Nov 2001: For complementation tests, amph26 homozygoteswere crossed to Sin3A/CyO mutant stocks, and non-curlyprogeny were assessed for amph26 mutant phenotypes.

15 May 2007: To directly test the. role of each residue of the IVF motif, in the context of. ... Methods. Errors bars were obtained by calculating the standard error and relevance of the difference assessed with a Student t-test.

23 May 2012: Wewere unable to test Trc35 in this assaybecause we could not purify Trc35 dueto poor expression and solubility. ... To test the involvementof the Bag6 complex in this process, we treated permeabilizedA9 cells with high salt, which removed most

30 Jul 2002: To test this hypothesis, HeLa cellswere fixed and stained for Eps15 (Figures 4A and 4B)and both AP1 and AP2 (Figures 4C and 4D) using anantibody against 1- and 2-adaptins.

1 May 2004: At least theparC/ParR/M system is simple enoughto test this possibility in vitro.

16 Jul 2008: To test the involvement of thispathway in PrP degradation by A4 cells, we analyzed PrPmetabolism upon inhibition of ERAD at two distinct stepswith either a proteasome inhibitor (MG132) or mannosidaseI inhibitor ... To test this idea, we generated a

19 Dec 2016: crenactin filaments. To test this, we performed. the same experiment using a peptide spanning.

7 Mar 2012: The reason for this is probably that the C-terminal amphipathic helix needs to be in contact with the membrane for polymerisation to occur at test-tube concentrations and in the ... Obviously, there are limitations as to what is possible, determined by

17 Mar 2003: In order to test the validity of this hypoth-by six residues to complete the b strand, resulted in a.

30 Apr 2010: 2. Layer this onto 100 µL of the sucrose cushion in an ultra-centrifuge tube (either a tube for the TLA120.1 rotor or amicro-test tube for the TL100.3 ... 1. Using glass measuring tools (e.g., glass pipettes), transfer20 mg PC and 5 mg PE to a glass

6 Feb 2003: To test whether the tetrameric TRAP complex alone wasthe component that stimulates. ... To test this, we analyzed theTRAP dependence of two point mutations in the PrP signalsequence (termed N7 and N9 [Fig.

21 Feb 2011: Science 321, 1354–1357. 62 Sever, S. et al. (2000) Garrotes, springs, ratchets, and whips: puttingdynamin models to the test.

12 Jan 2012: V3000 has not beentested yet. If somebody wants to test please let me know.

16 Oct 2003: To test this, we fused GFP or YFP to thecytoplasmic domains of three resident ER membrane proteinswith minimal cytoplasmic domains: two rough ER com-ponents of the translocon, Sec61. ... chimera. To test if OSER-inducing, GFP-tagged ER proteinswere

20 Mar 2014: Petal.Width and systematically higher Sepal.Width thanthe other two species, although to draw such conclusions we would have to test suchhypotheses properly).

30 Jul 2013: twist around the tube axis). Preliminary tests using a segment size similar to that in Fig.

30 May 2008: Experiments must now be designed to test this hypothesis. Translocational pausing has been viewed as a mecha- nism by which particular regions of the nascent chain can be isolated from the

21 Sep 2006: Meanwhile, the most definitive test for prions, a bioassay, was be-ing performed in parallel on comparable samples3639. ... These toolscan then be used in vivo to test specific hypotheses regarding the patho-genesis of prion diseases.

2 Feb 2004: 4) have been used asassembly tests for other members of the dynamin family(see Oligomerization web site in Online links). ... This clathrin-centricmodel for endocytosis does not, however, take intoaccount the role of dynamins in

29 Dec 2004: In order to test whether stimulated [3H]bumetanide binding would be re- tained in membranes after activation of transport by agents other than catecholamines, we examined specific binding in membranes from

22 Jul 2007: In addition,. using mouse models as a preclinical test, as we have done in.

24 Jan 2005: The model-derivedphases provided a more sensitive test of the signalretained in the images at high resolution than theprevious reference, which had been derived solelyfrom the images.

20 Nov 1997: Although the signifi-. cance of these findings remains to be determined, the Indeed, the contradicting conclusions resulting frommany of the studies which each used different test sub-rearrangements of the

7 Jul 1999: The residuals betweenthe tted absorbance and the experimental values wereplotted, to test for the validity of the model used.

3 Dec 2004: test-ed by sedimentation assays using liposomesor lipid tubules (19, 27, 28). ... Each assay contained0.05 mg/ml protein. (B) Liposomes containing 10%cholesterol, 40% phosphatidylethanolamine, 40%phosphatidylcholine, and 10% of a test lipid wereprepared

21 Nov 2012: Oncogene. Correlation of lipid kinase activity with lipid bindingTo test whether lipid binding forms the basis of p110activation, we compared lipid kinase with lipid-bindingactivities for three sets of p110a/p85a

20 Mar 2014: Petal.Width and systematically higher Sepal.Width thanthe other two species, although to draw such conclusions we would have to test suchhypotheses properly).

27 Apr 2015: To test for seeding, we followed filament formation onimmobilized TubRC complexes (Fig.

31 Mar 2014: This essentially tests whether the. Tutorial 6: Linear Regression 4. model has any predictive power. ... b) Create a model to test this hypothesis, assuming a linear relationshipbetween Height and Girth.

30 Jan 2004: T-tubule net-work (7 ). To test if this tubulating ability is dueto the Drosophila amphiphysin BAR domain,we mutated pairs of basic residues to gluta-mates, Lys161 Lys163 on the ... S3B). As a functional test of the amphiphysin BARdomain, we tested the

10 Jan 2013: Test set size (%) 5.4. Rwork (%) 19.6(22.9). Rfree (%) 25.2(23.8). ... Significance was calculated using the Student’s t test( = p,0.05). D: Confocal micrographs of RAM cells over-expressing Bin2-EGFP (Bin2, green, left) or control (EGFP, green, right).

26 Jun 2001: The present work has generated a wide arrayof tools to test this and other hypotheses in future studies.

23 Oct 2012: parenthesis. † Rmerge = ΣΣ | Ihl - 〈Ih〉 | / ΣΣ 〈Ih〉 h l h l ‡ 5 % of the reflections were included in the test set.

28 Nov 2008: The error bars show 95% confidence intervals (calculated by t tests) for each condition.

4 Aug 2017: Fig. 2. Reconstitution ofUBE2O-mediated clientubiquitination with purifiedfactors. (A) Experimentalstrategy to test UBE2O inter-action and ubiquitination in adefined system.

17 Mar 2014: Compare means to test for differences. • Consider variance (and shape) of the distributions – help choose appropriate prior/protocol. •

23 Jul 2007: To further test this notion, we performed two independent. measurements. First, we monitored by SPR analysis the.

7 Apr 2010: FEBS Letters 584 (2010) 1830–1839. j o u r n a l h o m e p a g e : w w w. F E B S L e t t e r s. o r g. Review. Modeling membrane shaping by proteins: Focus on EHD2 and N-BAR domains. Felix Campelo a,b, Gur Fabrikant a, Harvey T. McMahon c,