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  1. Results that match 1 of 2 words

  2. https://www2.mrc-lmb.cam.ac.uk/microscopes4schools/downloads/protocols

    https://www2.mrc-lmb.cam.ac.uk/microscopes4schools/downloads/M4S_activities.pdf
    11 Mar 2022: Lugol solution is a solution of iodine and potassium iodide in water and it is used as an indicator test for the presence of starches, with which it reacts by turning
  3. KWRM_A_992668_O

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-3.pdf
    20 Oct 2015: provides a critical test for functionalimportance. Indeed, optogenetic techni-ques to artificially manipulate neuronalactivity at high spatio-temporal resolutionhave been transformative for neurosci-ence.12,13 In a similar manner, techniquesto
  4. The MORPHEUS protein crystallization screen

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/morpheus%202009.pdf
    12 Nov 2009: Manydivalent cations and some carboxylic acids were discarded inlater tests because of problems with stability and false posi-tives. ... 3. Results and discussion. Both well known test proteins and novel samples were triedwith MORPHEUS.
  5. doi:10.1016/j.jbbm.2005.12.008

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/rfcloning2006%20published.pdf
    19 Apr 2006: TA. Fig. 2. Protein expression and solubility tests. A. Once transformed to BAI cells, protein expression was tested in 10 ml. ... B. Test expression of. the 136 kDa BSSMC protein at 25 8C in C41 cells (lane 2) and BAI cells (lanes 4).
  6. Clay_thesis

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/AnthonyKempf.pdf
    4 Oct 2005: receptors. To test this possibility, we performed ablations that eliminated the egg-laying. ... laying interval times, a non-parametric test was used (the Mann-Whitney rank test).
  7. JFCregg_OGS_finaledit

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/Cregg_thesis.pdf
    14 Dec 2010: eScholarship provides open access, scholarly publishingservices to the University of California and delivers a dynamicresearch platform to scholars worldwide. Electronic Theses and DissertationsUC San Diego. Peer Reviewed. Title:Pharmacological
  8. 554 Research Paper Inhibition of receptor-mediated endocytosis by the …

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/wigge_McMahon1997.pdf
    21 Mar 2003: To test whether the cellulartarget of amphiphysin is dynamin, COS-7 cells were cotransfected with bothdynamin and the amphiphysin SH3 domain; here, transferrin uptake wasefficiently rescued. ... To test the hypothesis that the SH3 domain ofamphiphysin is
  9. doi:10.1016/j.molcel.2004.08.030

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Haering%20Mol%20Cell%202004.pdf
    20 Sep 2004: However, the disorder makes it difficult todesign specific mutants to test this hypothesis.its C terminus (Figure 1D). ... To test whether the S525N mutation Is There Any Turnover of Cohesin Subunits.
  10. 318-320 News Feat Curves MH.indd

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/NatureAheadCurve.pdf
    17 Jul 2009: the artificially high concentrations present in the test tube. ... In the late 1990s, his graduate student Lars Dreier was mixing nuclear-envelope membranes with DNA, attempting to imitate in a test tube the way that the envelope reforms after cell
  11. 3747_0_supp_7_1052456676

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/christian.pdf
    9 May 2003: calcium concentration. To study the function of VGCCs we proceeded to test if. ... subunit UNC-36. Before proceeding to test VGCC mutants and subunit mutants we.
  12. Changes in Postural Syntax Characterize Sensory Modulation and…

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-5.pdf
    14 Aug 2015: A rank sum test comparingposture probabilities during and outside of stimulation periods shows that dorsal bends areover-represented (Fig 3C) during stimulation, as previously reported for this strain [21]. ... Hits are trigrams with significantly
  13. Identifying proteins bound to native mitotic ESC chromosomes reveals…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Djeghloul%20Nat%20Camms%202020.pdf
    11 Aug 2020: P-values of statistically significant increases, measured by unpaired two tailed Student’s t-tests, are indicated. ... P-values of statisticallysignificant decreases, measured by unpaired two tailed Student’s t-tests, are indicated.
  14. 73687_Trans

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/emboj201099s2.pdf
    28 Apr 2010: Therefore, if F17 does not insert into the membrane bilayer (see above) the authors should test possible effects of the F17V mutation on DivIVA higher-order oligomerization by analytical ultra centrifugation. ... It should be straightforward to test this
  15. 1 Supplementary data to accompany paper: Mechanism of endophilin ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndophilinEMBO/EndoSupMethods.pdf
    8 Jun 2006: simple gaussian functions to dc/dt versus s curves to test for the number of components and. ... Rfree test set size (%) 5.1 % outliers 0.5. <B> (Å2) 69.
  16. Tutorial 5: Hypothesis TestingRob Nicholls –…

    https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_statistics_course_2014/tutorials/hypothesis_testing.pdf
    26 Mar 2014: The F-test tests the null hypothesis that the variancesof two samples are equal using the test statistic:. ... 3 One-sample tests. One-sample t-test. The one-sample t-test tests the null hypothesis that the population mean is equalto some value µ0.
  17. Elementary hypothesis testing •  Introduction •  Some distributions…

    https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_statistics_course_2014/lectures/Lecture5_hypotheses.pdf
    7 Apr 2014: multiple hypothesis var.test - test for equality of variances power.t.test - to calculate power of t-test Some other tests. ... of two random variables are same Grubbs testtests for outliers (more in R package – outliers).
  18. Tutorial 5: Hypothesis TestingRob Nicholls –…

    https://www2.mrc-lmb.cam.ac.uk/download/tutorial-5-hypothesis-testing/?wpdmdl=18817&refresh=6693eb1d2ae181720970013
    26 Mar 2014: The F-test tests the null hypothesis that the variancesof two samples are equal using the test statistic:. ... 3 One-sample tests. One-sample t-test. The one-sample t-test tests the null hypothesis that the population mean is equalto some value µ0.
  19. The ER membrane protein complex promotes biogenesis of sterol-related …

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/JCS_2019.pdf
    18 Jan 2019: are shown (n=4). P0.01, P0.001 (Student’s t-test). 3. RESEARCH ARTICLE Journal of Cell Science (2019) 132, jcs223453. ... are shown (n=3).P0.001, P0.0001 (Student’s t-test). Reconstitution of Δ5 (D) and Δ6 cells (E) with empty vector control (EV),
  20. © 2005 Nature Publishing Group Endophilin and CtBP/BARS are ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndopohilinNature2005.pdf
    4 Jan 2006: Thus, a differentapproach is needed to test whether endophilin or CtBP/BARS doindeed have LPAAT activity.In a previous study1, the LPAAT activity of endophilin was. ... To test the possibility that LPAAT activity is not intrinsic toendophilin, we used a
  21. The structure of human thyroglobulin

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/TG_Nature2020.pdf
    31 Jan 2020: Nature, doi:10.1038/s41586-020-1995-4
  22. se060101051p

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/ford01.pdf
    3 Dec 2004: test-ed by sedimentation assays using liposomesor lipid tubules (19, 27, 28). ... Each assay contained0.05 mg/ml protein. (B) Liposomes containing 10%cholesterol, 40% phosphatidylethanolamine, 40%phosphatidylcholine, and 10% of a test lipid wereprepared
  23. Thesis Chapter 0

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/Hardaker.pdf
    10 Jun 2008: To test the possible involvement of FLP-1-encoded peptides in the modulation of egg-.
  24. MD1-91, MD1-92 and MD1-92-FX Morpheus II 10 mL, HT-96 and FX-96…

    https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB20.pdf
    9 Jul 2018: Figure 1. Examples of crystals obtained during initial tests with the final formulation of Morpheus II.
  25. MD1-46, MD1-47 and MD1-47-FX Morpheus 10 mL, HT-96 and FX-96…

    https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB17.pdf
    9 Jul 2018: Preliminary tests with Morpheus made within the Laboratory of Molecular Biology (LMB)1 at Cambridge, UK, have shown encouraging results with various targets.
  26. M410_8e 231..235

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Dynamin.pdf
    6 Mar 2001: In an effort to distinguish betweenthese two hypotheses, we designed mutants of dynamin that wouldenable us to test the relationship between dynamin's GTPase activityand its ability to support endocytosis. ... We then embedded cells asdescribed23. To
  27. httDownloaded from rsif.royalsocietypublishing.org ResearchCite this…

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-1.pdf
    4 Feb 2015: 2.12. Statistical analysisStandard statistical tests were performed using MATLAB func-tions. Correlation coefficients were calculated using theMATLAB function corrcoef. ... A value of þ1 indicates perfect positivecorrelation. A value of 21 indicates
  28. se060101051p

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsin/EM/ford.pdf
    3 Dec 2004: test-ed by sedimentation assays using liposomesor lipid tubules (19, 27, 28). ... Each assay contained0.05 mg/ml protein. (B) Liposomes containing 10%cholesterol, 40% phosphatidylethanolamine, 40%phosphatidylcholine, and 10% of a test lipid wereprepared
  29. Tutorial 6: Linear RegressionRob Nicholls –…

    https://www2.mrc-lmb.cam.ac.uk/download/tutorial-6-linear-regression/?wpdmdl=18818&refresh=6693eb1d693dc1720970013
    31 Mar 2014: This essentially tests whether the. Tutorial 6: Linear Regression 4. model has any predictive power. ... b) Create a model to test this hypothesis, assuming a linear relationshipbetween Height and Girth.
  30. Structure and superorganization of acetylcholinereceptor–rapsyn…

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnas13.pdf
    3 Jul 2013: 1,582 μm2;fitted skew normal distribution maximum: 5,522 μm2; n = 2,340;P < 0.001 by Mann–Whitney test), confirming the importance ofrapsyn for maintaining a high receptor ... The statistical significance of the dif-ference between those datasets was
  31. Influence of lipid bilayer on the structure of the muscle-­type…

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnasS24.pdf
    30 Apr 2024: 121 No. 19 e2319913121 https://doi.org/10.1073/pnas.2319913121 5 of 6. to test whether nanodisc reconstitution provides a means to recapit-ulate accurately the conformations of the receptor
  32. Supplementary Figure 1Lysophosphatidic acid acyl transferase (LPAAT)…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndoSupFigs.pdf
    4 Jan 2006: This software was also used for the direct fitting of simple gaussian functions to dc/dt versus s curves to test for the number of components and estimate their molecular mass.
  33. pgen.1004082 1..13

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/84_Chen_C_PlosGen_2014.pdf
    24 Sep 2015: indicatesp,0.01 by ANOVA, Bonferroni’s multiple comparisons test.(TIF). Figure S6 ODR-10-GFP localization in mutants expressingdefective rab proteins. ... p,0.01 (ANOVA, Bonferroni’s multiple comparisons test).(TIF). Figure S7 Deleting ufc-1 or uba-5
  34. 61 Ultramicroscopy 25 (1988) 279-292 North-HoUand, Amsterdam 279…

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ultram88.pdf
    1 Apr 2016: In this paper, we examine the behaviour of the CTF for thin ice-embedded specimens within the framework of the hnear theory of image forma-tion, using as a test object ... Test calcula-tions showed that the accuracy of this method is not sensitive to
  35. pnas201014665 19609..19610

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2010-Nogales-19609-10.pdf
    11 Nov 2010: 3). Just test your knowledge ofcytoskeleton structure by looking at theirfigure 4.
  36. pnas201313978 4601..4610

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/pnas2013duman.pdf
    23 Dec 2013: characteristic of membrane-binding amphipathichelices (30, 31).To test whether this amphipathic helix is able to recruit pro-. ... To test functionalities of the SepF mutants, the sepF mutationswere then introduced into a B.
  37. DOI: 10.1126/science.1136985 , 120 (2007); 316Science et al.Maria C.…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Alonso_Science.pdf
    6 Apr 2007: Fur-ther work will be required to test this prelim-inary interpretation. Existing data indicates that ATP binding hasprofound effects on the kinesin head, recon-figuring the active site and shifting
  38. se060101047p

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Itoh%20et%20al.pdf
    23 Nov 2004: MBP asa test substrate in a 50-ml reaction. ... Statistical comparisonswere made with the two-way analysis of variance(ANOVA) and two-tailed t test with Bonferroni cor-rection; P , 0.05 indicated statistical significance.
  39. Features critical for membrane binding revealed by DivIVA crystal…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/emboj201099a.pdf
    20 May 2010: membrane interaction. To test this, we purified the F17A. mutant and investigated membrane binding in vitro. ... could conveniently test suppressors for restored localization. The motivation for such screen was that suppressors of the.
  40. Membrane Localization is Critical for Activation of the PICK1 BAR…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/MadsenGetherTraffic2008.pdf
    18 Oct 2008: internalized to the observed clusters. To test this, we. performed an antibody feeding experiment using FLAG–. ... These values were significantlydifferent (p < 0.0001, unpaired t-test). B) COS-7 cells expressing.
  41. The structure of bactofilin filaments reveals their mode of membrane…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Deng2019Natmicro.pdf
    26 Aug 2019: Nature Microbiology, doi:10.1038/s41564-019-0544-0
  42. doi:10.1016/j.pbiomolbio.2004.07.009

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Publications/Robotic_nanolitre_protein_crystallisation.pdf
    22 Sep 2017: B) Aspiration of reagents from 15 ml test tubes held in a cooled rack. ... the desired chemical/buffer from 15ml test tubes held in arack on the robot platform.
  43. 53091 361..366

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/McMahonE.pdf
    3 Dec 2004: Liposome tubulation, sedimentation and electron microscopySynthetic liposomes contained 10% cholesterol, 40% phosphatidylethanolamine,40% phosphatidylcholine, and 10% of the test lipid (in most casesphosphatidylinositol(4,5)bisphosphate).
  44. pnas201206839 17424..17429

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/23045692.pdf
    21 Nov 2012: 2C and Fig. S2A, median abscission time of 70 min versus80 min for control treated cells; P < 0.0001, Mann–Whitneyu test). ... The differences in abscission times are significant (P < 0.0001) as determined by a Mann–Whitney u test.
  45. Twinning and other pathologies Andrey Lebedev CCP4 OD-­‐structures…

    https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_crystallography_course_2013/lectures/pathologies_and_twinning.pdf
    11 Jun 2013: Twinning tests summary. Space group validaAon. 04/06/2013 58 Crystallography Course LMB. ... Twinning tests summary. Space group validaAon. 04/06/2013 61 Crystallography Course LMB.
  46. doi:10.1016/j.cell.2006.03.035

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/16647110.pdf
    14 Jun 2006: pounds and also test the prediction that distinct regions. of the ATP binding pocket contribute to inhibitor selectivity. ... cell culture are recapitulated in vivo by performing insulin. tolerance tests in mice.
  47. doi:10.1016/j.str.2007.05.002

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/FCH02F-BARHenne07.pdf
    2 Jun 2007: protein saturation on liposomes. To test more directly whether the dimer seen in the crys-. ... tutive helix. To test for a role for helix 1 in membrane bind-.
  48. A Conserved Archaeal Pathway for TailAnchored Membrane Protein…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/70_Sherrill_J_Traffic_2011.pdf
    11 Nov 2011: To test this, we first determinedthe X-ray crystal structure of ADP•AlF4-bound TRC40from the methanogen Methanothermobacter thermau-totrophicus at 2.1 Å resolution (Table S1, Figure S1 andMethods). ... To test this, we used a protease-protection
  49. Twinning and other pathologies Andrey Lebedev CCP4 OD-­‐structures…

    https://www2.mrc-lmb.cam.ac.uk/download/twinningaandaotherapathologiesa-andreyalebedeva-a/?wpdmdl=18788&refresh=6693eb1a485511720970010
    11 Jun 2013: Twinning tests summary. Space group validaAon. 04/06/2013 58 Crystallography Course LMB. ... Twinning tests summary. Space group validaAon. 04/06/2013 61 Crystallography Course LMB.
  50. doi:10.1016/j.cell.2008.11.004

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/55_Kang_SW_Cell_2008.pdf
    21 Nov 2008: Exami-nation of differences between related species offers a glimpse at the history of evolutionary processes, but monitor-ing evolution in a test tube allows deeper exploration of these processes as
  51. 15591370691369 1..30

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/elife_2019.pdf
    29 May 2019: To test whether the role of TMED10 in GFP-PrP degradation depends on complex formation, we.

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