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The Structure of a Tunicate C-type Lectin from Polyandrocarpa…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/10398588.pdf7 Jul 1999: The residuals betweenthe tted absorbance and the experimental values wereplotted, to test for the validity of the model used. -
doi:10.1016/j.jmb.2004.12.031
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jmb05.pdf24 Jan 2005: The model-derivedphases provided a more sensitive test of the signalretained in the images at high resolution than theprevious reference, which had been derived solelyfrom the images. -
doi:10.1016/j.devcel.2004.09.003
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/15469844.pdf1 Nov 2004: An analogous salt link is formed betweenattractive proposal. To test this, we made an N-terminalArg83 of Vps25 and Asp548 of Vps36. -
Regulation of lipid binding underlies the activation mechanism of…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/22120714.pdf21 Nov 2012: Oncogene. Correlation of lipid kinase activity with lipid bindingTo test whether lipid binding forms the basis of p110activation, we compared lipid kinase with lipid-bindingactivities for three sets of p110a/p85a -
pone.0052401 1..15
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Podosome2012/journal.pone.0052401.pdf10 Jan 2013: Test set size (%) 5.4. Rwork (%) 19.6(22.9). Rfree (%) 25.2(23.8). ... Significance was calculated using the Student’s t test( = p,0.05). D: Confocal micrographs of RAM cells over-expressing Bin2-EGFP (Bin2, green, left) or control (EGFP, green, right). -
Reconstitution of a prokaryotic minus end-trackingsystem using TubRC…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2015-Fink-E1845-50.pdf27 Apr 2015: To test for seeding, we followed filament formation onimmobilized TubRC complexes (Fig. -
se060101051p
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/AP180/Ap180_st.pdf3 Dec 2004: test-ed by sedimentation assays using liposomesor lipid tubules (19, 27, 28). ... Each assay contained0.05 mg/ml protein. (B) Liposomes containing 10%cholesterol, 40% phosphatidylethanolamine, 40%phosphatidylcholine, and 10% of a test lipid wereprepared -
BAR Domains as Sensors ofMembrane Curvature: TheAmphiphysin BAR…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/PeterBARdomains.pdf30 Jan 2004: T-tubule net-work (7 ). To test if this tubulating ability is dueto the Drosophila amphiphysin BAR domain,we mutated pairs of basic residues to gluta-mates, Lys161 Lys163 on the ... S3B). As a functional test of the amphiphysin BARdomain, we tested the -
MeganPalm
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/MeganPalm.pdf3 Dec 2005: previously used for the egg detection test and 20 new normal wild type videos. ... type. To test whether unc-29(x29) was clustering in the correct region, data obtained. -
Tutorial 2: Descriptive Statisticsand Exploratory Data Analysis…
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_statistics_course_2014/tutorials/descriptive_statistics_and_exploratory_data_analysis_answers.pdf20 Mar 2014: Petal.Width and systematically higher Sepal.Width thanthe other two species, although to draw such conclusions we would have to test suchhypotheses properly). -
The structural basis of novel endosome anchoringactivity of KIF16B ...
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17641687.pdf23 Jul 2007: To further test this notion, we performed two independent. measurements. First, we monitored by SPR analysis the. -
BAR Domains as Sensors ofMembrane Curvature: TheAmphiphysin BAR…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/PeterBARdomains.pdf?ijkey=TikQO8xVkpUak&keytype=ref&siteid=sci30 Jan 2004: T-tubule net-work (7 ). To test if this tubulating ability is dueto the Drosophila amphiphysin BAR domain,we mutated pairs of basic residues to gluta-mates, Lys161 Lys163 on the ... S3B). As a functional test of the amphiphysin BARdomain, we tested the -
REPORT◥ PROTEOSTASIS UBE2O is a quality control factor fororphans ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017b.pdf4 Aug 2017: Fig. 2. Reconstitution ofUBE2O-mediated clientubiquitination with purifiedfactors. (A) Experimentalstrategy to test UBE2O inter-action and ubiquitination in adefined system. -
Modeling membrane shaping by proteins: Focus on EHD2 and N-BAR domains
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/KozlovEHDs.pdf7 Apr 2010: FEBS Letters 584 (2010) 1830–1839. j o u r n a l h o m e p a g e : w w w. F E B S L e t t e r s. o r g. Review. Modeling membrane shaping by proteins: Focus on EHD2 and N-BAR domains. Felix Campelo a,b, Gur Fabrikant a, Harvey T. McMahon c, -
Phosphoinositide-Specific Phospholipase Cδ1 Activity toward Micellar…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9287165.pdf20 Apr 2002: of theδ1 enzyme show similar structural features, it was of interestto test the mammalianδ1 enzyme for activation by organicsolvent. -
Structure and superorganization of acetylcholinereceptor–rapsyn…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnas13.pdf3 Jul 2013: 1,582 μm2;fitted skew normal distribution maximum: 5,522 μm2; n = 2,340;P < 0.001 by Mann–Whitney test), confirming the importance ofrapsyn for maintaining a high receptor ... The statistical significance of the dif-ference between those datasets was -
Molecular Biology of the CellVol. 19, 5093–5103, December 2008 ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/LynchMBC5093.pdf24 Jan 2009: significantly (p 0.002)from wild-type and Syt-1RK by a general linear model analysis of variance using the Tukey post hoc test of values. -
Tutorial 6: Linear RegressionRob Nicholls –…
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_statistics_course_2014/tutorials/linear_regression.pdf31 Mar 2014: This essentially tests whether the. Tutorial 6: Linear Regression 4. model has any predictive power. ... b) Create a model to test this hypothesis, assuming a linear relationshipbetween Height and Girth. -
Statistical Theory; Why is the Gaussian Distribution so popular? ...
https://www2.mrc-lmb.cam.ac.uk/download/statistical-theory-why-is-the-gaussian-distribution-so-popular-rob-nicholls/?wpdmdl=18809&refresh=669a8527ae4ba172140266317 Mar 2014: Compare means to test for differences. • Consider variance (and shape) of the distributions – help choose appropriate prior/protocol. • -
CAN-11-2008 3642..3651
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/79_Basseville_A_CanRes_2012.pdf24 Sep 2015: Statistical analysisThe Student t test was carried out for each experiment. -
Protection from cytosolic prion protein toxicityby modulation of…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/30_Rane_N_EMBO_2004.pdf8 Nov 2004: To test this possibility, we analyzed the behavior of PrP. constructs containing mutations in the transmembrane do-. -
Influence of lipid bilayer on the structure of the muscle-type…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnasS24.pdf30 Apr 2024: 121 No. 19 e2319913121 https://doi.org/10.1073/pnas.2319913121 5 of 6. to test whether nanodisc reconstitution provides a means to recapit-ulate accurately the conformations of the receptor -
pgen.1004082 1..13
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/84_Chen_C_PlosGen_2014.pdf24 Sep 2015: indicatesp,0.01 by ANOVA, Bonferroni’s multiple comparisons test.(TIF). Figure S6 ODR-10-GFP localization in mutants expressingdefective rab proteins. ... p,0.01 (ANOVA, Bonferroni’s multiple comparisons test).(TIF). Figure S7 Deleting ufc-1 or uba-5 -
61 Ultramicroscopy 25 (1988) 279-292 North-HoUand, Amsterdam 279…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ultram88.pdf1 Apr 2016: In this paper, we examine the behaviour of the CTF for thin ice-embedded specimens within the framework of the hnear theory of image forma-tion, using as a test object ... Test calcula-tions showed that the accuracy of this method is not sensitive to -
Relocalization of STIM1 for Activation of Store-operated Ca2!Entry Is …
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/47_Ong_HL_JBC_2007.pdf24 Sep 2015: previous report (15). We wanted to test whether a model withtwo ER compartments that are functionally distinct but slowlyequilibrate with each through diffusion of Ca2! -
Supplement_Final
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/81_Shao_S_Mol_Cell_2013.pdf16 May 2013: To test this idea, we produced RNCs ina ubiquitination-deficient lysate (Hessa et al., 2011) depleted ofthe requisite E2s and ubiquitin (Figure 4C). ... This suggests that their combinationprovides the specificity for Listerin recruitment. To test -
FtsA forms actin-like protofilaments
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/EMBO2012FtsAPiotr/emboj201276a.pdf27 Mar 2012: be the most reliable test for FtsA polymerization, as it shows. -
A positively charged channel within the Smc1/Smc3 hinge required for…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embosmcjingekurze2010.pdf1 Dec 2010: generate sister chromatid cohesion. To test the effect of charge neutralization on cohesin complex. -
Structural Mapping of the Catalytic Mechanism for a…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9048554.pdf20 Apr 2002: Data statistics are listed in Table1. Test data for the calculation of freeR factors of all thesedata sets were chosen to be consistent with the selection oftest data for the native -
Membrane Protein Insertion at the Endoplasmic Reticulum
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/75_Shao_S_Ann_Rev_2011.pdf9 Nov 2011: The apparentfree-energy contribution of each amino acidin any test sequence was found to be additive,with the sum determining the final insertionprobability. ... earlier transmembrane domains ( gray), and a later test sequence(red ) is measured for -
Science Journals — AAAS
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2023/08/Science_2021.pdf26 Aug 2021: p values for a two-tailedStudent’s t test comparison to Q1are indicated. -
Cryo‐EM structure of the MinCD copolymeric filament from Pseudomonas…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Szewczak-Harris_et_al-2019-FEBS_Letters.pdf22 Jul 2019: by MinD’s MTS. To test whether P. aeruginosa MinC and MinD pro-. -
554 Research Paper Inhibition of receptor-mediated endocytosis by the …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/wigge_McMahon1997.pdf?cmd=Retrieve&db=PubMed&list_uids=9348539&dopt=Abstract21 Mar 2003: To test whether the cellulartarget of amphiphysin is dynamin, COS-7 cells were cotransfected with bothdynamin and the amphiphysin SH3 domain; here, transferrin uptake wasefficiently rescued. ... To test the hypothesis that the SH3 domain ofamphiphysin is -
Twinning and other pathologies Andrey Lebedev CCP4 OD-‐structures…
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/%22https:/www2.mrc-lmb.cam.ac.uk/download/twinningaandaotherapathologiesa-andreyalebedeva-a/?wpdmdl=18788&11 Jun 2013: Twinning tests summary. Space group validaAon. 04/06/2013 58 Crystallography Course LMB. ... Twinning tests summary. Space group validaAon. 04/06/2013 61 Crystallography Course LMB. -
tra_578 1..18
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Burtey%20IVF%20Traffic%2007.pdf15 May 2007: To directly test the. role of each residue of the IVF motif, in the context of. ... Methods. Errors bars were obtained by calculating the standard error and relevance of the difference assessed with a Student t-test. -
A Ubiquitin Ligase-Associated Chaperone Holdase Maintains…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/72_Wang_Q_Mol_Cell_2011.pdf23 May 2012: Wewere unable to test Trc35 in this assaybecause we could not purify Trc35 dueto poor expression and solubility. ... To test the involvementof the Bag6 complex in this process, we treated permeabilizedA9 cells with high salt, which removed most -
Amphiphysin is necessaryfor organization of the…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/DroxAmph.pdf9 Nov 2001: For complementation tests, amph26 homozygoteswere crossed to Sin3A/CyO mutant stocks, and non-curlyprogeny were assessed for amph26 mutant phenotypes. -
2623.tif
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb90.pdf23 Jan 2012: Three-dimensional Structure of the Acetylcholine Receptor by Cryoelectron Microscopy and Helical Image Reconstruction C h i k a s h i T o y o s h i m a a n d N i g e l U n w i n. Medical Research Council Laboratory of Molecular Biology, Cambridge -
PII: S0969-2126(02)00801-8
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Kent_McMahonOwen2002.pdf30 Jul 2002: To test this hypothesis, HeLa cellswere fixed and stained for Eps15 (Figures 4A and 4B)and both AP1 and AP2 (Figures 4C and 4D) using anantibody against 1- and 2-adaptins. -
Molecular Biology of the CellVol. 19, 3463–3476, August 2008 ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/51_Ashok_A_MBC_2008.pdf16 Jul 2008: To test the involvement of thispathway in PrP degradation by A4 cells, we analyzed PrPmetabolism upon inhibition of ERAD at two distinct stepswith either a proteasome inhibitor (MG132) or mannosidaseI inhibitor ... To test this idea, we generated a -
30 Apr 2004 18:9 AR AR214-BB33-09.tex AR214-BB33-09.sgm…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/annrev2004.pdf1 May 2004: At least theparC/ParR/M system is simple enoughto test this possibility in vitro. -
80096 RPF
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/EMBO2012FtsAPiotr/emboj201276s3.pdf7 Mar 2012: The reason for this is probably that the C-terminal amphipathic helix needs to be in contact with the membrane for polymerisation to occur at test-tube concentrations and in the ... Obviously, there are limitations as to what is possible, determined by -
Chapter 20 In Vitro Dissection of Protein Translocationinto the ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/62_Sharma_AS_MMB_2010.pdf30 Apr 2010: 2. Layer this onto 100 µL of the sucrose cushion in an ultra-centrifuge tube (either a tube for the TLA120.1 rotor or amicro-test tube for the TL100.3 ... 1. Using glass measuring tools (e.g., glass pipettes), transfer20 mg PC and 5 mg PE to a glass -
14821680963433 1..18
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/e21600-download.pdf19 Dec 2016: crenactin filaments. To test this, we performed. the same experiment using a peptide spanning. -
The Jour nal o f Cel l Bio logy ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/24_Fons_RD_JCB_2003.pdf6 Feb 2003: To test whether the tetrameric TRAP complex alone wasthe component that stimulates. ... To test this, we analyzed theTRAP dependence of two point mutations in the PrP signalsequence (termed N7 and N9 [Fig. -
088542u047
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Owen_McMahon1999.pdf17 Mar 2003: In order to test the validity of this hypoth-by six residues to complete the b strand, resulted in a. -
Expression, purifica0on, characteriza0on, and crystalliza0on So how…
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/%22https:/www2.mrc-lmb.cam.ac.uk/download/03-protein-expression-crystallization-and-mutagenesis-meindert-lamers/?wpdmdl=18793&2 May 2013: Beware of the layer of cold air over the liquid nitrogen (2) Test crystal at room temp: Mitegen crystal sleeve: MicroRT Test different cryos & precipitant concentra-ons Remove any mother liqour -
Combinatorial Control of Prion Protein Biogenesis by the…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/18_Kim_SJ_JBC_2001.pdf26 Jun 2001: The present work has generated a wide arrayof tools to test this and other hypotheses in future studies. -
The GTPase-Activating Protein GRAF1 Regulates the CLIC/GEEC Endocytic …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Lundmark_McMahon_GRAF2008.pdf28 Nov 2008: The error bars show 95% confidence intervals (calculated by t tests) for each condition. -
Wild card macros
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/sciencegayathri2012/Gayathri.SM.pdf23 Oct 2012: parenthesis. † Rmerge = ΣΣ | Ihl - 〈Ih〉 | / ΣΣ 〈Ih〉 h l h l ‡ 5 % of the reflections were included in the test set.
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