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Calcium-mediated Evidence from the Changes in Gap Junction Low ...
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb83.pdf1 Nov 2012: Acid-washed glassware was used in experiments designed to test sensitivity o f gap j u n c t i o n structure to divalent cations. -
Volume 22 May 15, 2011 1625 Cytosolic aggregates perturb ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/69_Chakrabarti_O_MBC_2011.pdf11 Nov 2011: The aggregated protein indicated at left was coexpressed with the test protein indicated along the top. ... At each time point, the % of aggregate-containing cells that showed excess intracellular accumulation of the test protein is indicated. -
pone.0107211 1..10
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/leoa2014.pdf3 Sep 2014: DLPs. We attempted to test this hypothesis directly but were. unable to obtain either individually, in combination, or expressed. -
Single‐dose immunisation with a multimerised SARS‐CoV‐2 receptor…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/COVID%20vaccine%202021%201873-3468.14171.pdf29 Aug 2021: outside of the sphere. We aimed to test whether Dps could efficiently dis-. ... Spike-S-Dps sera gave very little neutralisation. Whilst. pseudoviruses are widely used to test the neutralisation. -
MR_LMB
https://www2.mrc-lmb.cam.ac.uk/download/molecular-replacement/?wpdmdl=18799&refresh=669a8523c6fc9172140265911 Jun 2013: approximate • conformational change. • Test multiple choices of model • easier in a pipeline: phenix.MRage, Balbes, MrBUMP • multiple templates,. -
doi:10.1128/mBio.00800-13. 4(6): .mBio. Midcell DNA Translocase FtsK…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mbio2013bisicchia.pdf23 Dec 2013: To test whether the translocase is responsiblefor promoting and maintaining the hexameric state of FtsK invivo, we determined the stoichiometry of an FtsK derivative lack-ing the C-terminal domain (FtsKC; -
ANALYSIS Integrating molecular and network biologyto decode…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Schmid_Nature2007.pdf22 Aug 2007: We would suggest that a good test for proteins that function ashubs only when clustered in space and time is that overexpression ofsuch a protein should not have any phenotype on -
PII: S1097-2765(02)00515-4
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/SMC%20cohesin%20paper%202002.pdf19 Apr 2002: To test whether Smc3 s head alone is sufficient. to bind Scc1, we created an artificial head in which25 min, whereas the on rate of Smc3 was about twicethat of its ... To test this directly, we repeated the aboveexperiment using a yeast strain expressing -
JCB Article The Journal of Cell Biology, Volume 164, ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/28_Snapp_EL_JCB_2004.pdf15 Apr 2004: Each bar represents the mean SD (n 10), with statistically significant differences (P 0.01 using t test) being observed between the two conditions. ... multiple days.aTreatment is significant (P 0.01) compared with untreated cells using t test. -
doi:10.1016/j.neuron.2005.05.004
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Jockusch_Neuron2005.pdf17 Jun 2005: To test if a peptide de-rived from rat amphiphysin would inhibit the amphi-physin-AP2 interaction in goldfish, we performed abinding experiment using a GST-tagged construct of ratamphiphysin 1 ... ease of vesicles that were not primed at the time -
DOI: 10.1126/science.1256917, 963 (2014);346 Science et al.Thomas G.…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Science-2014-Gligoris-963-7.pdf21 Nov 2014: S7E). Sister DNAs are entrapped bycohesin trimers. To test whether cross-linking all three interfacesof the cohesin ring entraps sister DNAs in a co-valent topological embrace, we isolated dimersof a -
MR_LMB
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_crystallography_course_2013/lectures/MR_LMB.pdf11 Jun 2013: approximate • conformational change. • Test multiple choices of model • easier in a pipeline: phenix.MRage, Balbes, MrBUMP • multiple templates,. -
Dbouk 1..13
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/vadas_science_signaling.pdf5 Dec 2012: To test the biological relevance of Gbg-mediated activation in p110bsignaling, we compared the ability of wild-type and mutant p110bconstructs to mediate cellular transformation and motility. ... To test the role of p110b-Gbginteractions in PTEN-null -
x16412u141
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/MaoAP180-2001.pdf17 Mar 2003: test set of reflections, consisting of a randomly selected 5% of the data and not used during refinement. -
doi:10.1016/S0076-6879(05)04052-8
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/HigginsMcMahonMethods2005.pdf26 Jan 2006: 1E)(Ford et al., 2002). As many other proteins are predicted to containN‐terminal amphipathic helices, the liposome tubulation assay will provea valuable test for the folding and insertion of these -
Structure of the hexagonal surface layer on Caulobacter crescentus…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/s-layer-nmicrobiol201759.pdf6 Apr 2017: The model was improved manually using MAIN(ref. 38) and refined with PHENIX and REFMAC (refs 39, 40), keeping the 5%randomly selected test set of reflections separate at all times. -
doi:10.1016/j.molcel.2006.07.020
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/HiroseMC2006.pdf8 Sep 2006: In tests for MT. binding, the Kar3 protein showed a Kd of 0.37 6 0.10 mM with no. -
PII: S1534-5807(04)00100-5
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Johannes_DevCell2004.pdf22 Mar 2005: In contrast,EE/RE, was inhibited in CHC RNAi-treated cells. Welarge amounts of STxB/HRP were found in clathrin-con-used quantitative biochemical techniques to test thistaining early endosomal vacuoles ... To test whether AP1 is required for the retro- -
Supplementary Text & Figs
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Schmid_Nature2007Sup.pdf22 Aug 2007: sufficient overexpression to test if the network can be distorted and overexpressed. -
Cooperative Recruitment of Dynamin and BIN/Amphiphysin/Rvs (BAR)…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/JBC_Meinecke2013.pdf19 Mar 2013: 3C). To further test for auto-inhibition, we per-formed a series of co-sedimentation assays with SUVs (Fig. ... 42). Although our assay does notallow us to test for possible scission mechanisms, we can ask thefollowing question. -
Statistical Theory; Why is the Gaussian Distribution so popular? ...
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_statistics_course_2014/lectures/Lecture4_Statistical_Theory.pdf17 Mar 2014: Compare means to test for differences. • Consider variance (and shape) of the distributions – help choose appropriate prior/protocol. • -
2168 | K. von der Malsburg et al. Molecular ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/93_vonderMalsburg-_K_MBC_2015.pdf11 Aug 2015: To test this directly, we used nondenaturing affinity purification to assess a possible 60S-listerin-translocon ternary complex (Figure 1E). ... To test this, we treated cultured cells with either the transla-tion elongation inhibitor cycloheximide (CHX) -
LETTERS Structural basis for selective recognition of ESCRT-IIIby the …
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17928861.pdf8 Dec 2007: To test whether MIMs have a role in sorting, we mutated motifs inboth Vps2 and Did2. -
Expression, purifica0on, characteriza0on, and crystalliza0on So how…
https://www2.mrc-lmb.cam.ac.uk/download/03-protein-expression-crystallization-and-mutagenesis-meindert-lamers/?wpdmdl=18793&refresh=669a852400fdd17214026602 May 2013: Beware of the layer of cold air over the liquid nitrogen (2) Test crystal at room temp: Mitegen crystal sleeve: MicroRT Test different cryos & precipitant concentra-ons Remove any mother liqour -
Bacterial actin MreB assembles in complex with cell shape protein RodZ
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embo2010RodZMreB.pdf17 Feb 2010: B) MreB polymerisation assays wereused to test the interface mutants of RodZ for MreB binding. -
early in DNA damage response and regulateRAP80 localization. Many ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/MartensSytPap07.pdf16 Oct 2009: Future geneticstudies will allow us to test this possibility. References and Notes1. -
PII: S0304-3991(97)00070-3
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ultram97.pdf4 Apr 2003: The best estimate was taken to be the defocus yielding the highest image Quality, Qi, de- fined as the number of test phases in agreement with (Iphase difference1 < 90’) the centrosymmetric ... 13). To test how sensitively PQ measures the signal-to- -
Functional Depletion of Mahogunin by Cytosolically Exposed Prion…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/59_Chakrabarti_O_Cell_2009.pdf13 Jun 2009: an in vivo interaction assay based on coassociation of an. FP-tagged test protein with FP-tagged cytosolic PrP aggregates. ... involving excessive PrP exposure to the cytosol. To test this. idea, we analyzed the status of Mgrn and lysosomes in trans-. -
A substrate-specific inhibitor of proteintranslocation into the…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/35_Garrison_JL_Nature_2005.pdf15 Jul 2005: 2e). To test whether this difference underlies cotransin’ssubstrate selectivity, we prepared chimaeric constructs in which thesignals of VCAM1 and pPrl were fused to an otherwise non-translocated reporter. ... 3a), these results suggestthat cotransin -
Molecular Replacement Practical IntroductionDuring the practical you…
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_crystallography_course_2013/tutorials/MolecularReplacementPractical.pdf29 Aug 2012: The crystal structure of the complex between BETA and BLIP has been a test case for molecular replacement because of the difficulty encountered in the original structure solution. -
mmi_4133.fm
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Spo0J%202004.pdf30 Jun 2004: parS. sequence5. ¢. -TGTTCCACGTGAAACA-3. ¢. (Fig. 1A and B). To test forthe ability of wild-type and truncated proteins to carry outDNA binding and spreading, we used an agarose gel -
doi:10.1016/j.tibs.2009.03.001
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/58_Chakrabarti_O_TIBS_2009.pdf13 Jun 2009: It will then be possible to test in transgenicmice whether the effects of the HD mutant stem solely fromCtmPrP production or through other mechanisms (e.g. ... PrP gener-ation, trafficking and degradation so that they can bemanipulated selectively to -
PHAGOCYTES, GRANULOCYTES, AND MYELOPOIESIS PSTPIP2 deficiency in mice …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Blood-2012-Chitu-3126-35.pdf12 Oct 2012: Imageswere obtained with a cooled EMCCD camera (9100-02; Hamamatsu). Statistics. Significance was tested using the 2-tailed Student t test. ... To test the impact of PSTPIP2-deficiency disease in mice onosteolytic responses in vivo, we performed -
PNAS202120006_proof
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/pnas%20Lee%202022.pdf18 Dec 2022: To test thesehypotheses, we used the same N-terminal Brn1 release assay(Fig. -
Roles of Amphipathic Helices and the Bin/Amphiphysin/Rvs(BAR) Domain…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Jao_JBC2010.pdf4 Oct 2010: Thus, our first goalwas to test whether the overall structure of the endophilin BARdomain is retained upon membrane interaction and, if so, howits concave surface interacts with the membrane. ... To test for potential movement between thecentral region -
The Hydrophobic Insertion Mechanism of Membrane Curvature Generation…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/CampeloBioPJ2008.pdf13 Dec 2008: 5). To test the sensitivity of the model. predictions to this assumption, we repeated the calculations. -
No Job Name
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/vandenent2008ftsq.pdf4 Mar 2008: Expression tests were done inBl21AI cells (Invitrogen) and C41 cells (Miroux and Walker,1996) with a 10 ml culture and purified over a Ni2 NTA spincolumn as described previously (van den -
Arginine 383 is a crucial residue in ABCG2 biogenesis
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/56_Polgar_O_BBA_2009.pdf13 Jun 2009: 2.4. Northern blotting. RNA was extracted from cells using RNA STAT-60 (Tel-Test Inc.,Friendswood, TX). ... 2A). To test the functionality of the R383A and R383G mutants, flowcytometry was performed after incubating cells with the ABCG2-substrates -
doi:10.1016/j.str.2008.06.010
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/18786397.pdf3 Sep 2008: some uncertainty of the molecular interpretation of the filaments,. so we carried out site-specific mutagenesis to test the contacts. -
The Jour nal o f Cel l Bio logy ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/26_Snapp_EL_JCB_2003.pdf16 Oct 2003: To test this, we fused GFP or YFP to thecytoplasmic domains of three resident ER membrane proteinswith minimal cytoplasmic domains: two rough ER com-ponents of the translocon, Sec61. ... chimera. To test if OSER-inducing, GFP-tagged ER proteinswere -
Protein-driven membrane stresses in fusion and fission
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/KozlovCurvature2010.pdf21 Feb 2011: Science 321, 1354–1357. 62 Sever, S. et al. (2000) Garrotes, springs, ratchets, and whips: puttingdynamin models to the test. -
refmac keywords
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/refmac/refmac_keywords.pdf12 Jan 2012: V3000 has not beentested yet. If somebody wants to test please let me know. -
Qrb1300006 1..40
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/qrb13.pdf30 Jul 2013: twist around the tube axis). Preliminary tests using a segment size similar to that in Fig. -
Asymmetric distribution of pause transfer sequences in apolipoprotein …
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/6_Kivlen_MH_JLR_1997.pdf30 May 2008: Experiments must now be designed to test this hypothesis. Translocational pausing has been viewed as a mecha- nism by which particular regions of the nascent chain can be isolated from the -
SVUS002-16.tex
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/31_Rane_Chapter_2005.pdf21 Sep 2006: Meanwhile, the most definitive test for prions, a bioassay, was be-ing performed in parallel on comparable samples3639. ... These toolscan then be used in vivo to test specific hypotheses regarding the patho-genesis of prion diseases. -
Tutorial 2: Descriptive Statisticsand Exploratory Data Analysis…
https://www2.mrc-lmb.cam.ac.uk/download/tutorial-2-descriptive-statistics-2/?wpdmdl=18820&refresh=669a85273addb172140266320 Mar 2014: Petal.Width and systematically higher Sepal.Width thanthe other two species, although to draw such conclusions we would have to test suchhypotheses properly). -
Membrane transport between compartments in eukary-otic cells requires …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Dynaminreview2004.pdf2 Feb 2004: 4) have been used asassembly tests for other members of the dynamin family(see Oligomerization web site in Online links). ... This clathrin-centricmodel for endocytosis does not, however, take intoaccount the role of dynamins in -
THE JOURNAL OF BIOLOGICAL CHEMISTRY 0 1990 by The ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/1_Pewitt_EB_JBC_1990a.pdf29 Dec 2004: In order to test whether stimulated [3H]bumetanide binding would be re- tained in membranes after activation of transport by agents other than catecholamines, we examined specific binding in membranes from -
Tumour prevention by a single antibody domaintargeting the…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17568777.pdf22 Jul 2007: In addition,. using mouse models as a preclinical test, as we have done in. -
Cell, Vol. 91, 575–582, November 28, 1997, Copyright 1997 ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/9_Hegde_RS_Cell_1997.pdf20 Nov 1997: Although the signifi-. cance of these findings remains to be determined, the Indeed, the contradicting conclusions resulting frommany of the studies which each used different test sub-rearrangements of the
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