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  2. LETTERS Architectural and mechanistic insights into an EHDATPase…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/DaumkeMcMahonEHDNature/Daumke_McMahon_EHD.pdf
    28 Sep 2007: later). Following the middle domain, a40-residue linker connects the helical domain with the C-terminalEH-domain (residues 443–543). ... S, supernatant; P, pelleted fraction.d, Electron micrographs of negatively stainedPtdSer liposomes in the absence
  3. Modeling membrane shaping by proteins: Focus on EHD2 and N-BAR domains

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/KozlovEHDs.pdf
    7 Apr 2010: As a result, a membranefragment with an amphipathic helix in the middle has an effectivefunnel-like shape, which can fit to a sphere with a certain radiusand the corresponding total curvature.
  4. Protein-driven membrane stresses in fusion and fission

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Kozlov%20Trends%20Biochem%20Sci%202010.pdf
    20 Nov 2010: The fusion pathway consists of. membrane dimpling leading to the generation of curvature stresses and the establishment of point-like intermembrane contacts (left panel), fusion stalk formation (middle. ... Science 321, 1354–1357. 62 Sever, S. et al.
  5. The ER membrane protein complex is a transmembrane domain insertase

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017c.pdf
    27 Jan 2018: MEMBRANE TARGETING. The ER membrane protein complex isa transmembrane domain insertaseAlina Guna,1 Norbert Volkmar,2 John C. Christianson,2 Ramanujan S. Hegde1†. Insertion of proteins into membranes is an essential cellular process. The
  6. Wild card macros

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/sciencegayathri2012/Gayathri.SM.pdf
    23 Oct 2012: parenthesis. † Rmerge = ΣΣ | Ihl - 〈Ih〉 | / ΣΣ 〈Ih〉 h l h l ‡ 5 % of the reflections were included in the test set. ... The middle segments (initial seed) of the filaments become photobleached as the movie progresses, while the intensity
  7. 2168 | K. von der Malsburg et al. Molecular ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/93_vonderMalsburg-_K_MBC_2015.pdf
    11 Aug 2015: To test this directly, we used nondenaturing affinity purification to assess a possible 60S-listerin-translocon ternary complex (Figure 1E). ... NCtRNA. NC. 87654321. 87654321. 654321. (middle) E1 and E2 were analyzed by SDS–PAGE and autoradiography.
  8. Functional Depletion of Mahogunin by Cytosolically Exposed Prion…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/59_Chakrabarti_O_Cell_2009.pdf
    13 Jun 2009: an in vivo interaction assay based on coassociation of an. FP-tagged test protein with FP-tagged cytosolic PrP aggregates. ... involving excessive PrP exposure to the cytosol. To test this. idea, we analyzed the status of Mgrn and lysosomes in trans-.
  9. The GTPase-Activating Protein GRAF1 Regulates the CLIC/GEEC Endocytic …

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Lundmark_McMahon_GRAF2008.pdf
    28 Nov 2008: The error bars show 95% confidence intervals (calculated by t tests) for each condition. ... top), internalized anti-GFP (middle), and a merged, triple-labeled image (bottom). Arrows indicate points of colocalization.
  10. elife.elifesciences.org MacKinnon et al. eLife 2014;3:e01483. DOI:…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/83_MacKinnon_AL_eLife_2014.pdf
    9 Sep 2015: Comparison of sensitive and resistant TMDs at multiple points along the integration pathway (i.e., at multiple nascent chain lengths) should allow us to: (1) test whether they pass through a
  11. cdf519 5057..5068

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/12356722.pdf
    12 Oct 2002: Within the p47phox-PX PtdIns(3,4)P2-binding site,Arg43 [the middle residue of the conserved (R/K)-(R/K)-(F/Y) motif located in the b3/a1 loop] ... To test this notion, wemeasured the binding of mutants to vesicles contain-ing
  12. Cryo‐EM structure of the MinCD copolymeric filament from Pseudomonas…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Szewczak-Harris_et_al-2019-FEBS_Letters.pdf
    22 Jul 2019: by MinD’s MTS. To test whether P. aeruginosa MinC and MinD pro-. ... pole oscillation of a protein required for directing. division to the middle of Escherichia coli.
  13. https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/13500

    https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/13500
    {"id":13500,"date":"2018-12-21T11:09:54","date_gmt":"2018-12-21T11:09:54","guid":{"rendered":"https://www2.mrc-lmb.cam.ac.uk/?page_id=13500"},"modified":"2023-07-21T12:53:12","modified_gmt":"2023-07-21T11:53:12","slug":"lmb-365","status":"publish","t
  14. Influence of lipid bilayer on the structure of the muscle-­type…

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnasS24.pdf
    30 Apr 2024: 1 A, Middle) and a symmetrical rim of MX helices lying flat against the inner (cytoplasmic) bilayer surface (Fig. ... B) In-plane slabs through densities and superimposed model cutting through the top, middle and bottom portions of the TM helices.
  15. supplementary figures 070814 JL

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/scc32014si.pdf
    8 Aug 2014: and Zr Scc3. (A) Zr Scc3 structure, same as Fig. 1A, middle, colored blue to red from N- to C-. ... flow through, b: bound). (B) To test if Sc Scc3 forms homodimers, full length.
  16. Blocking action of intestinal immune cell enhances the immune…

    https://www2.mrc-lmb.cam.ac.uk/blocking-action-of-intestinal-immune-cell-enhances-the-immune-response-to-colorectal-cancer/
    Thumbnail for Blocking action of intestinal immune cell enhances the immune response to colorectal cancer - MRC Laboratory of Molecular Biology 7 Jun 2022: squares). Cancers from the transgenic APC mouse (left) are larger than those found in the APC mice that lack IL-25 (middle) or ILC2s (right). ... Further tests on human CRC tissue, provided by Simon Buczacki at Addenbrooke’s Hospital, Cambridge (now at
  17. Features critical for membrane binding revealed by DivIVA crystal…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/emboj201099a.pdf
    20 May 2010: membrane interaction. To test this, we purified the F17A. mutant and investigated membrane binding in vitro. ... could conveniently test suppressors for restored localization. The motivation for such screen was that suppressors of the.
  18. Impress

    https://www2.mrc-lmb.cam.ac.uk/coot/ephemera/beyond-coloured-lines.pdf
    28 Sep 2020: see the video– Ctrl <middle-mouse> scroll. 4 / 50. “3D sticks in space” is the way in which Coot 0.0-0.9.1 has represented models and maps. ... 28 / 50. Atoms Clashing. Test-bed/Hello World. Ribosomes at 60FPS. Ribosomes at 60FPS.
  19. Dissertation

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/AndyHuang.pdf
    6 Mar 2008: 74. Table 4.5: The results of the significance test (p-value < 0.05 is considered to be significant).
  20. Membrane Localization is Critical for Activation of the PICK1 BAR…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/MadsenGetherTraffic2008.pdf
    18 Oct 2008: eYFP–PICK1 wt (middle row) or to-. gether with eYFP–PICK1 3KE (bottom. ... green (middle column). Right column. shows the merged pictures. White. bar 10 mm.
  21. Anne Bertolotti: Finding clues to combat neurodegenerative disease -…

    https://www2.mrc-lmb.cam.ac.uk/life-at-the-lmb/profiles/anne-bertolotti-finding-clues-to-combat-neurodegenerative-disease/
    Thumbnail for Anne Bertolotti: Finding clues to combat neurodegenerative disease - MRC Laboratory of Molecular Biology 21 Jul 2023: she says inadvertently became a test for how the LMB would perceive someone who wanted to be both a mother and a scientist. ... We don’t just want junior or middle-ranking women, we need women in leadership positions, and we still have a long way to go
  22. The Coot User Manual

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/docs/coot.html
    1 Mar 2021: The Coot User Manual
  23. Using Coot for model building into EM maps:A tutorial ...

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/EM-Tutorial-Coot-PE.pdf
    8 Sep 2015: The test case for the tutorial is F420-reducing NiFe hydrogenase (Frh) {Allegretti:2014tz}. ... As a test case, we are going to use chain A from Frh.
  24. LMB 365 - Day 31 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-31%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... was officially handed over on 29 January 1962, and all 43 scientists had moved in by the middle of February.
  25. LMB 365 - Day 64 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-64%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... elegans (and eggs), with red and green fluorescence marking different tissues in the body, including neurons in the head
  26. LMB 365 - Day 53 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-53%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... 200),s.height=r):"link"===t.message&&(r=new URL(s.getAttribute("src")),n=new
  27. LMB 365 - Day 137 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-137%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... 200),s.height=r):"link"===t.message&&(r=new URL(s.getAttribute("src")),n=new
  28. LMB 365 - Day 221 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-221%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... 200),s.height=r):"link"===t.message&&(r=new URL(s.getAttribute("src")),n=new
  29. LMB 365 - Day 146 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-146%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... 200),s.height=r):"link"===t.message&&(r=new URL(s.getAttribute("src")),n=new
  30. LMB 365 - Day 167 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-167%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... over on 29 January 1962, and all 43 scientists had moved in by the middle of February.
  31. LMB 365 - Day 166 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-166%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... was officially handed over on 29 January 1962, and all 43 scientists had moved in by the middle of February.
  32. LMB 365 - Day 165 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-165%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... over on 29 January 1962, and all 43 scientists had moved in by the middle of February.
  33. LMB 365 - Day 163 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-163%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... over on 29 January 1962, and all 43 scientists had moved in by the middle of February.
  34. LMB 365 - Day 168 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-168%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... over on 29 January 1962, and all 43 scientists had moved in by the middle of February.
  35. Phil. Trans. Roy. Soc. Lond. B. 261, 95-104 (1971) ...

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ptrs71.pdf
    26 Nov 2012: electron diffraction pattern: for the weakly scattering material we wish to examine, the high intensity of the central order beam would ensure that the potential at the middle of the thread ... The requirement now is that the potential at the middle of
  36. Bacterial actin MreB assembles in complex with cell shape protein RodZ

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embo2010RodZMreB.pdf
    17 Feb 2010: B) MreB polymerisation assays wereused to test the interface mutants of RodZ for MreB binding. ... structures from whichfilaments grow when mixed together in the presence of nucleotide and MgCl2 (middle panel).
  37. 2623.tif

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb90.pdf
    23 Jan 2012: Sections at the horizontal line near the middle of the tube are shown in Fig. ... The ridges are quite prominent near the membrane but broad and smooth near the synaptic end; surface features are more complicated in the middle region.
  38. LMB 365 - Day 149 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-149%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... The supporting metal helices in the middle of the stem have some parts where the nucleotide bases are left out,
  39. 61 Ultramicroscopy 25 (1988) 279-292 North-HoUand, Amsterdam 279…

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ultram88.pdf
    1 Apr 2016: In this paper, we examine the behaviour of the CTF for thin ice-embedded specimens within the framework of the hnear theory of image forma-tion, using as a test object ... correspond to 0.1 fim (left and middle) and 1/50 A " ' (right).
  40. MinCD cell division proteins form alternating copolymeric cytomotive…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/ncomms6341.pdf
    6 Dec 2014: 1a,d). MinC and MinD copolymerize to form an alternatingcopolymer. To experimentally test filament formation, we usedfull-length, untagged E. ... A. Rapid pole-to-pole oscillation of a proteinrequired for directing division to the middle of Escherichia
  41. Coot Scripting Interface: cc-interface.hh Source File

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/docs/html/cc-interface_8hh_source.html
    24 Aug 2021: 615 // test the returned spec for unset_p().
  42. PNAS202120006_proof

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/pnas%20Lee%202022.pdf
    18 Dec 2022: To test thesehypotheses, we used the same N-terminal Brn1 release assay(Fig. ... 4. (A, Left and Middle) Condensin pentamer clamping circular plasmid DNA, as observed by cryo-EM with a VPP.
  43. Insight into bacterial cell division: Architecture of the FtsZ ring - …

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Finsight-bacterial-cell-division-architecture-ftsz-ring%2F&format=xml
    t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... n=new
  44. Membrane transport between compartments in eukary-otic cells requires …

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Dynaminreview2004.pdf
    2 Feb 2004: Sequence homology does not allow us todefine the region between the middle domain and the. ... In humanMxA, a single point mutation (L612K) in the GEDblocks the interaction with the middle domain andinhibits oligomerization74.
  45. The structure of bactofilin filaments reveals their mode of membrane…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Deng2019Natmicro.pdf
    26 Aug 2019: Left, magnifications of the corners of a. Middle, magnifications of the corners of b with the TtBac sequence added beneath. ... coli. N. N. N. N. WT. Liposome. BactofilinBactofilin. Liposome. WT: middle of cell WT: top of cell.
  46. Thesis Chapter 0

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/Hardaker.pdf
    10 Jun 2008: To test the possible involvement of FLP-1-encoded peptides in the modulation of egg-.
  47. Qrb1300006 1..40

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/qrb13.pdf
    30 Jul 2013: twist around the tube axis). Preliminary tests using a segment size similar to that in Fig. ... 7b). As will be. described in Section 4.3, the gate of the channel is located near the middle of the membrane-.
  48. 30 Apr 2004 18:9 AR AR214-BB33-09.tex AR214-BB33-09.sgm…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/annrev2004.pdf
    1 May 2004: FtsZ is the organizingmolecule of bacterial cell division and forms a filamentous ring around the middle ofthe cell. ... At least theparC/ParR/M system is simple enoughto test this possibility in vitro.
  49. doi:10.1016/j.molcel.2008.05.027

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsK%20MolCell%202008%20Lowe.pdf
    16 Aug 2008: generally further away from the DNA with many water molecules. mediating contacts with the DNA (Figures 1E, middle, S1D, and. ... sequently activate recombination. In order to test whether the dimeric forms of the protein had.
  50. PII: S1097-2765(02)00515-4

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/SMC%20cohesin%20paper%202002.pdf
    19 Apr 2002: Upper two rows, open V conformation; middle two rows, Yconformation; lower row, coils spread conformation. ... To test this directly, we repeated the aboveexperiment using a yeast strain expressing Scc1myc18Cohesin Contains Only a Single Molecule.
  51. pone.0107211 1..10

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/leoa2014.pdf
    3 Sep 2014: tween the classical dynamin middle domain and GTPase effector. domain (GED) (Figure 1B). ... per other DFMs) a predicted membrane binding domain between. a middle domain and a GTPase effector domain (GED).

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