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{"id":19090,"date":"2020-09-03T15:00:35","date_gmt":"2020-09-03T13:00:00","guid":{"rendered":"https://www2.mrc-lmb.cam.ac.uk/?page_id=19090"},"modified":"2023-07-21T12:51:37","modified_gmt":"2023-07-21T11:51:37","slug":"anne-bertolotti-finding-clues-

29 Apr 2024: We showed that FtsK forms a hexamer, with the DNA going through the middle of the ring. ... The middle section, after the membrane anchor, is unfolded, possibly providing a spacer to the cell wall through periplasm.

26 Sep 2011: What is endophilin and how does it work. Research on the mechanism of endophilin in rapid vesicle endocytosis. Research on proteins involved in clathrin-coated vesicle formation. Research on membrane bending. Research on protein-lipid interactions.

19 Jun 2024: Libcootapi’s Documentation. Libcootapi is a new (as of 2022) interface to the functionality of Coot. It is designed to be (is intended to be) a clean, consistent and easy-to-use interface primarily targeting WebAssembly/JavaScript and secondarily,

26 Sep 2011: Overexpression of endocytic proteins in COS cells to test the effects of mutations in vivo. ... Liposome/lipid nanotube sedimentation assays to test lipid binding and specificity. (also immunoprecipitations, cloning, kinase assays, etc.).

26 Sep 2011: More experiments need to be done to test this. The platinum shadowing (from a single angle) shows that AP180 does not induce curvature in clathrin assemblies (left panel).

26 Sep 2011: Isothermal Titration Calorimetry is used to make accurate measurements of affinities and stoichiometries of interactions. It is based on fine measurements of heat changes when ligands interact

20 Mar 2023: An insert in the middle of the BAR domain, defines an endophilin/nadrin subfamily of BAR domains, where part of this insert forms another short amphipathic helix and also contributes to ... We then collaborated with Peter Cullen (Bristol University) to

26 Sep 2011: Researching the reason immune responses are sprroessed during Yersinia infections.

{"id":23084,"date":"2022-06-07T10:17:27","date_gmt":"2022-06-07T09:17:27","guid":{"rendered":"https://www2.mrc-lmb.cam.ac.uk/?p=23084"},"modified":"2022-06-07T10:17:29","modified_gmt":"2022-06-07T09:17:29","slug":"blocking-action-of-intestinal-immune

8 Aug 2019: Pan the view so that the middle of the map is at the middle of the screen. ... Test, play, refine, yank until satisfied. Reset Geman-McClure alpha to 1.

20 Oct 2020: Move back to the middle of the molecule map. Cryo-EM Go To Map Molecule Middle. ... Try proportial editing: with the Real Space Refinement active, use Ctrl Middle-mouse scroll to change the radius of the atoms affected by the atom pull displacement.

21 Jul 2023: During the last year, over 4,500 initial crystallisation screens were used at the LMB to test macromolecular samples of interest. ... This image for day 263 of #LMB365 shows a test spectrum created using precision sensors and software.

24 Aug 2021: analyse_map_point_density_change (const std::vector< int > &map_number_list). test function for analysis of multiple map. ... map to model density statistics, reported per residue, the middle residue of a range of residues More..

31 Mar 2021: An x-ray beam will follow the hole down the middle of a capillary tube so you can move the tube about and aim it for the crystal. ... More recently, I’ve worked with Richard Henderson and Greg McMullan on various high vacuum test rigs for the new breed

20 Oct 2015: 3A, middle).We were thus able to introduce a qualita-tive modification to the C. ... We inserted an electrical syn-apse between AWC and AIY (Fig. 3B,middle).

3 Dec 2004: endogenous AP2 (middle, AP6 antibody) and transferrin uptake (right). A strong. ... Liposome tubulation, sedimentation and electron microscopySynthetic liposomes contained 10% cholesterol, 40% phosphatidylethanolamine,40% phosphatidylcholine, and 10% of

11 Jun 2013: Twinning tests summary. Space group validaAon. 04/06/2013 58 Crystallography Course LMB. ... Twinning tests summary. Space group validaAon. 04/06/2013 61 Crystallography Course LMB.

8 Dec 2007: Leu 64Vps4 and Glu 68Vps4 form key interactions withArg 224Vps2 in the middle of aC (Fig. ... To test whether MIMs have a role in sorting, we mutated motifs inboth Vps2 and Did2.