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{"id":19090,"date":"2020-09-03T15:00:35","date_gmt":"2020-09-03T13:00:00","guid":{"rendered":"https://www2.mrc-lmb.cam.ac.uk/?page_id=19090"},"modified":"2023-07-21T12:51:37","modified_gmt":"2023-07-21T11:51:37","slug":"anne-bertolotti-finding-clues-

29 Apr 2024: We showed that FtsK forms a hexamer, with the DNA going through the middle of the ring. ... The middle section, after the membrane anchor, is unfolded, possibly providing a spacer to the cell wall through periplasm.

26 Sep 2011: What is endophilin and how does it work. Research on the mechanism of endophilin in rapid vesicle endocytosis. Research on proteins involved in clathrin-coated vesicle formation. Research on membrane bending. Research on protein-lipid interactions.

19 Jun 2024: Libcootapi’s Documentation. Libcootapi is a new (as of 2022) interface to the functionality of Coot. It is designed to be (is intended to be) a clean, consistent and easy-to-use interface primarily targeting WebAssembly/JavaScript and secondarily,

26 Sep 2011: Overexpression of endocytic proteins in COS cells to test the effects of mutations in vivo. ... Liposome/lipid nanotube sedimentation assays to test lipid binding and specificity. (also immunoprecipitations, cloning, kinase assays, etc.).

26 Sep 2011: More experiments need to be done to test this. The platinum shadowing (from a single angle) shows that AP180 does not induce curvature in clathrin assemblies (left panel).

26 Sep 2011: Isothermal Titration Calorimetry is used to make accurate measurements of affinities and stoichiometries of interactions. It is based on fine measurements of heat changes when ligands interact

20 Mar 2023: An insert in the middle of the BAR domain, defines an endophilin/nadrin subfamily of BAR domains, where part of this insert forms another short amphipathic helix and also contributes to ... We then collaborated with Peter Cullen (Bristol University) to

26 Sep 2011: Researching the reason immune responses are sprroessed during Yersinia infections.

{"id":23084,"date":"2022-06-07T10:17:27","date_gmt":"2022-06-07T09:17:27","guid":{"rendered":"https://www2.mrc-lmb.cam.ac.uk/?p=23084"},"modified":"2022-06-07T10:17:29","modified_gmt":"2022-06-07T09:17:29","slug":"blocking-action-of-intestinal-immune

8 Aug 2019: Pan the view so that the middle of the map is at the middle of the screen. ... Test, play, refine, yank until satisfied. Reset Geman-McClure alpha to 1.

20 Oct 2020: Move back to the middle of the molecule map. Cryo-EM Go To Map Molecule Middle. ... Try proportial editing: with the Real Space Refinement active, use Ctrl Middle-mouse scroll to change the radius of the atoms affected by the atom pull displacement.

21 Jul 2023: During the last year, over 4,500 initial crystallisation screens were used at the LMB to test macromolecular samples of interest. ... This image for day 263 of #LMB365 shows a test spectrum created using precision sensors and software.

24 Aug 2021: analyse_map_point_density_change (const std::vector< int > &map_number_list). test function for analysis of multiple map. ... map to model density statistics, reported per residue, the middle residue of a range of residues More..

31 Mar 2021: An x-ray beam will follow the hole down the middle of a capillary tube so you can move the tube about and aim it for the crystal. ... More recently, I’ve worked with Richard Henderson and Greg McMullan on various high vacuum test rigs for the new breed

20 Oct 2015: 3A, middle).We were thus able to introduce a qualita-tive modification to the C. ... We inserted an electrical syn-apse between AWC and AIY (Fig. 3B,middle).

3 Dec 2004: endogenous AP2 (middle, AP6 antibody) and transferrin uptake (right). A strong. ... Liposome tubulation, sedimentation and electron microscopySynthetic liposomes contained 10% cholesterol, 40% phosphatidylethanolamine,40% phosphatidylcholine, and 10% of

11 Jun 2013: Twinning tests summary. Space group validaAon. 04/06/2013 58 Crystallography Course LMB. ... Twinning tests summary. Space group validaAon. 04/06/2013 61 Crystallography Course LMB.

8 Dec 2007: Leu 64Vps4 and Glu 68Vps4 form key interactions withArg 224Vps2 in the middle of aC (Fig. ... To test whether MIMs have a role in sorting, we mutated motifs inboth Vps2 and Did2.

18 Dec 2008: 1C, middle panel). Immunostain-ing with antibodies to Saci1373 (ESCRT-III). revealed a distinct subcellular localization ofthe protein. ... To further test the invivo role of the Sulfolobus ESCRTcomponents, wegenerated an episomal vector to direct

11 Jun 2013: Twinning tests summary. Space group validaAon. 04/06/2013 58 Crystallography Course LMB. ... Twinning tests summary. Space group validaAon. 04/06/2013 61 Crystallography Course LMB.

13 Dec 2008: inclusion reaching its middle (Fig. 4 b) will not result in themembrane bending since the upper monolayer can expand. ... 5). To test the sensitivity of the model. predictions to this assumption, we repeated the calculations.

16 Jul 2023: S1E). As a further test of thisfinding, we expressed the Vps4E209Q plasmid in a cell line carryinga thymidine kinase homolog (hereafter called STK). ... 3A).To test the functional significance of this patterned assembly ofESCRT-III polymers, we used

22 Jul 2007: F-actin stress fibres (Figure 2E, middle panel compared with. uninfected cells or cells infected with retrovirus expressing. ... In addition,. using mouse models as a preclinical test, as we have done in.

3 Dec 2005: previously used for the egg detection test and 20 new normal wild type videos. ... in 5 sec CANGCRMAX Max angle change rate in middle sect.TLMV10AVG Avg.

24 Jan 2005: Theinner M2 helices also change their configuration inresponse to ACh, widening the lumen of the pore atthe middle of the membrane. ... The model-derivedphases provided a more sensitive test of the signalretained in the images at high resolution than

27 Mar 2012: and Errington, 2009). FtsZ finds the middle of the cell. through two independent mechanisms in E. ... colicells and imaged by electron cryotomography. EcFtsA without the amphipathic helix (top left) forms long, straight bundles in the middle of thecell

14 Jun 2006: Middle) Plot of the similarity between PI3-Ks according to their IC50 values against small molecule. ... cell culture are recapitulated in vivo by performing insulin. tolerance tests in mice.

30 Jun 2004: parS. sequence5. ¢. -TGTTCCACGTGAAACA-3. ¢. (Fig. 1A and B). To test forthe ability of wild-type and truncated proteins to carry outDNA binding and spreading, we used an agarose gel ... Groups of 12 scans were taken shortly after theboundary cleared

12 Oct 2012: Imageswere obtained with a cooled EMCCD camera (9100-02; Hamamatsu). Statistics. Significance was tested using the 2-tailed Student t test. ... Pseudocolored fluorescence image ofCOS-7 cells expressing EGFP alone (left), WT EGFP-PSTPIP2 (middle) or

4 Oct 2007: tion. Mutant parR genes were tested for functionality in their. natural genetic context on test plasmids derived from low-. ... As shown in Figure 4A, test plasmids. containing the ParR171 K6E, R7S and K8E mutations severely.

21 Nov 2014: Scc1 contains many more residues in the middle domain. Separase cleavage sites, Pds5(7) and Scc3 binding sites are highlighted (28). ... S7E). Sister DNAs are entrapped bycohesin trimers. To test whether cross-linking all three interfacesof the cohesin

15 Mar 2018: thetic complex. To test this hypothesis, we examined MreB motions as we changed the shape of. ... increase with cell width (Ouzounov et al., 2016). To test if the angle of MreB movement changes.

18 Feb 2016: Thus, the idea emerged that mRNAs are ‘tested’ for their integrity by translation, and failure of this test initiates their degradation to prevent production of faulty proteins1,31. ... An advantage of encoding an arrest-inducing sequence in the

9 Nov 2001: Testing them will involve addressing ques-tions such as the interactions that localize T-tubules tothe middle of the M–Z interval, the binding partners ofthe SH3 domain of Drosophila amphiphysin ... For complementation tests, amph26 homozygoteswere

19 Dec 2016: helical 2D class average (top), re-projections of F-actin double-stranded filament structure (middle) (von der Ecken et al., 2015) and crenactin single. ... crenactin filaments. To test this, we performed. the same experiment using a peptide spanning.

11 Nov 2011: The aggregated protein indicated at left was coexpressed with the test protein indicated along the top. ... At each time point, the % of aggregate-containing cells that showed excess intracellular accumulation of the test protein is indicated.

21 Feb 2011: To test these activities, wecoexpressed full-length Rvs161 and Rvs167 using an E. ... To-gether, our biochemical tests show that Rvs161-Rvs167 bindsto membranes and induces three-dimensional curvature.

17 Jun 2005: ease of vesicles that were not primed at the time theeptide was introduced.To test whether a population of vesicles were primed. ... irst stimulus (upper), the percentage of excess membrane re-rieved during the fast phase (middle), and the rate constant

15 Apr 2004: The left and middle panels used directly conjugated anti-CNX antibodies against the COOH and NH2 terminus, respectively. ... multiple days.aTreatment is significant (P 0.01) compared with untreated cells using t test.

8 Jun 2006: implying the formation of additional helical structures. To test. how the predicted N-terminal amphipathic helix folds,. ... seeResults). Student’s t-test Po0.001, Po0.2. Endophilin and vesicle buddingJL Gallop et al. &

22 Mar 2005: EpsinR was localized to endosomal cells that were recognized via thick tubular elements inmembranes, and involved in the retrograde transport of their cytoplasm (Figure 1G, middle). ... To test whether AP1 is required for the retro- dress its function in

20 Jun 2009: Horizontal black barsindicate the mean values for each data set. Each mutant dataset was compared to wtPrP by the Student’s t-test and found to be statisticallysignificant in all cases

17 Mar 2003: of polymerized clathrin triskelia making a lattice of hexa-gons and pentagons, a middle layer where clathrin and To whom correspondence should be addressed (e-mail: hmm@. ... In order to test the validity of this hypoth-by six residues to complete the b

21 Feb 2011: middle of the stalk and contributes similarly to theenergies of both fusion and fission stalks [3,73].[(Box_1)TD$FIG]. ... Science 321, 1354–1357. 62 Sever, S. et al. (2000) Garrotes, springs, ratchets, and whips: puttingdynamin models to the test.

10 Jan 2013: A side view is shown in the middle and a view of theconcave surface on the bottom. ... Test set size (%) 5.4. Rwork (%) 19.6(22.9). Rfree (%) 25.2(23.8).

3 Sep 2008: Second, a molecule from the A layer of one. strand uses its a1/a2 loop to contact the middle of helix a2 of. ... some uncertainty of the molecular interpretation of the filaments,. so we carried out site-specific mutagenesis to test the contacts.

4 Aug 2017: middle) or physical interactions (bottom). ... Fig. 2. Reconstitution ofUBE2O-mediated clientubiquitination with purifiedfactors. (A) Experimentalstrategy to test UBE2O inter-action and ubiquitination in adefined system.

7 Jul 1999: The middle sphere lies between the side-chains of Asp2 of one molecule in the asymmetricunit and a symmetry-related copy of the other mol-ecule in the asymmetric unit. ... The residuals betweenthe tted absorbance and the experimental values wereplotted,