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  2. Identifying proteins bound to native mitotic ESC chromosomes reveals…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Djeghloul%20Nat%20Camms%202020.pdf
    11 Aug 2020: P-values of statistically significant increases, measured by unpaired two tailed Student’s t-tests, are indicated. ... P-values of statisticallysignificant decreases, measured by unpaired two tailed Student’s t-tests, are indicated.
  3. 1 Supplementary data to accompany paper: Mechanism of endophilin ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndophilinEMBO/EndoSupMethods.pdf
    8 Jun 2006: simple gaussian functions to dc/dt versus s curves to test for the number of components and. ... amphipathic helix are disordered, as well as a loop in the middle of the first helix which.
  4. A-nsmb855.indd

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nsmb%20FtsZ%202004.pdf
    19 Nov 2004: 2). The break in the protofilament is necessary for crystallization because the dimer formed by the 43-Å interaction (middle of Fig. ... To test for bound nucleotide, FtsZ was precipitated using 0.5 N cold HClO4, and after centrifugation nucleotide
  5. Molecular Biology of the CellVol. 8, 2003–2015, October 1997 ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/WiggeKohler_McMahon1997.pdf
    10 Oct 1997: To test this directly, Amph2 immunoprecipi-tates were Coomassie-stained, revealing that Amph1. ... This provided us with a useful tool to test theimportance of the heterodimer in vivo.
  6. doi:10.1016/j.cell.2006.01.047

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/16615893.pdf
    18 Apr 2006: through an adaptor protein. To test whether these two. complexes can directly interact in vitro, we used analyt-. ... Avanti or Echelon). Control vesicles contained an additional 10% PC. instead of the test lipid.
  7. doi:10.1016/j.str.2007.05.002

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/FCH02F-BARHenne07.pdf
    2 Jun 2007: protein saturation on liposomes. To test more directly whether the dimer seen in the crys-. ... tutive helix. To test for a role for helix 1 in membrane bind-.
  8. A positively charged channel within the Smc1/Smc3 hinge required for…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embosmcjingekurze2010.pdf
    1 Dec 2010: form a pseudo twofold symmetric torus with a small channel. in the middle. ... generate sister chromatid cohesion. To test the effect of charge neutralization on cohesin complex.
  9. doi:10.1016/j.molcel.2004.08.030

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Haering%20Mol%20Cell%202004.pdf
    20 Sep 2004: However, the disorder makes it difficult todesign specific mutants to test this hypothesis.its C terminus (Figure 1D). ... To test whether the S525N mutation Is There Any Turnover of Cohesin Subunits.
  10. JFCregg_OGS_finaledit

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/Cregg_thesis.pdf
    14 Dec 2010: Toledo, Ohio, in the middle of winter and, finally, to San Francisco. ... After the. UK was a stop in Toledo, Ohio, in the middle of winter, not my top choice of places to.
  11. x16412u141

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/MaoAP180-2001.pdf
    17 Mar 2003: test set of reflections, consisting of a randomly selected 5% of the data and not used during refinement. ... The segments (colored yellow) at the C-terminal end of a7 helix and the middle of a8 helix are engaged in an intramolecularantiparallel leucine
  12. Supplementary Text & Figs

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Schmid_Nature2007Sup.pdf
    22 Aug 2007: GTP domain, middle domain, GED, PH, PRD, no clathrin binding sites, maybe adaptor binding via a few motifs. ... no addit. Dynamins (excluding Dynamin like and mitofusins). XP_001183998, similar to Dynamin 2 GTP domain, middle domain, PH, GED, but no PRD,
  13. Cooperative Recruitment of Dynamin and BIN/Amphiphysin/Rvs (BAR)…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/JBC_Meinecke2013.pdf
    19 Mar 2013: 3C). To further test for auto-inhibition, we per-formed a series of co-sedimentation assays with SUVs (Fig. ... 42). Although our assay does notallow us to test for possible scission mechanisms, we can ask thefollowing question.
  14. Twinning and other pathologies Andrey Lebedev CCP4 OD-­‐structures…

    https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/%22https:/www2.mrc-lmb.cam.ac.uk/download/twinningaandaotherapathologiesa-andreyalebedeva-a/?wpdmdl=18788&
    11 Jun 2013: Twinning tests summary. Space group validaAon. 04/06/2013 58 Crystallography Course LMB. ... Twinning tests summary. Space group validaAon. 04/06/2013 61 Crystallography Course LMB.
  15. doi:10.1016/j.devcel.2008.06.015

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/54_Rane_NS_DevCell_2008.pdf
    18 Sep 2008: In this study, we have. performed experiments to test these predictions. ... After crosslinking, the products were fractionated into membrane-associated and. lumenal proteins, shown in the left and middle panels, respectively.
  16. coot-user-manual.dvi

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/docs/coot-user-manual.pdf
    28 Sep 2012: If you set it, Coot will test each ’:’-separatedstring that it points to a directory, and if it does, Coot will load all the. ... Rotate View around Screen Z axis. Middle-mouse. Centre on atom. Scroll-wheel Forward.
  17. ProSHADE: src/proshade/ProSHADE_saveLoad.cpp Source File

    https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/d0/df1/_pro_s_h_a_d_e__save_load_8cpp_source.html
    1 Nov 2018: ProSHADE 0.6.5 (NOV 2018). Protein Shape Descriptors and Symmetry Detection. ProSHADE_saveLoad.cpp. Go to the documentation of this file.. 1. 21 //============================================ RVAPI. 22 #include <rvapi_interface.h>. 23. 24 //=========
  18. Supplement_Final

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/81_Shao_S_Mol_Cell_2013.pdf
    16 May 2013: To test this idea, we produced RNCs ina ubiquitination-deficient lysate (Hessa et al., 2011) depleted ofthe requisite E2s and ubiquitin (Figure 4C). ... This suggests that their combinationprovides the specificity for Listerin recruitment. To test
  19. Abstract_Book_HMM_v2

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/conferences/JM_Abstract_Book.pdf
    15 Sep 2011: MOLECULAR BASIS FOR MEMBRANE REMODELLING  AND ORGANIZATION . Analyses moléculaires de l'organisation et du remodelage des membranes. President: Harvey McMAHON MRC Laboratory of Molecular Biology, Neurobiology Division,.
  20. Membrane Protein Insertion at the Endoplasmic Reticulum

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/75_Shao_S_Ann_Rev_2011.pdf
    9 Nov 2011: The apparentfree-energy contribution of each amino acidin any test sequence was found to be additive,with the sum determining the final insertionprobability. ... earlier transmembrane domains ( gray), and a later test sequence(red ) is measured for
  21. Transmembrane topogenesis of a tail-anchoredprotein is modulated by…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/36_Brambillasca_S_EMBO_2005.pdf
    20 Jul 2005: test this hypothesis, we analysed b5-Nglyc translocation. into protein-free liposomes of differing composition.
  22. Molecular Biology of the CellVol. 16, 279 –291, January ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/32_Levine_CG_MBC_2005.pdf
    21 Dec 2004: Differences among eachconstruct were statistically significant (p 0.01 by Student’s t test).(B) Ten nanograms per well of constructs encoding the indicatedsignal sequences fused to TF were analyzed for
  23. 16262551036386 1..32

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/cohesin%20folding%202021%20elife-67268-v1.pdf
    14 Jul 2021: decreasing it with Pds5. To test this, we measured the effect of scc4-4 in the presence or absence of.
  24. JCB: Article The Rockefeller University Press $30.00J. Cell Biol. ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/J%20Cell%20Biol%202010%20Howes.pdf
    24 Aug 2011: A single section of the original tomogram is shown (left). Various rotations of a 3D contoured electron density render were generated (middle). ... 6 F). To test the requirement for CLIC endocytosis during mi-gration, this inactivation assay was applied
  25. doi:10.1016/j.str.2008.05.003

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/52_Menetret_JF_Structure_2008.pdf
    22 Jul 2008: We. analyzed subsets of the final data set to test this idea. ... low the tunnel exit (Figure 7, middle). Subsequent disassocia-. tion of the SRP-SR complex from the ribosome would precede.
  26. 15955888524538 1..29

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2020/09/eLife_2020b.pdf
    24 Jul 2020: To test this idea, we monitored translation output from a reporter mRNA containing a collision-. ... Hegde, 2017). This precluded a clear test of EDF1’s role using this stalling sequence.
  27. A unified evolutionary origin for the ubiquitous protein transporters …

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2023/08/BMC_Biology_2021.pdf
    15 Dec 2021: To test the sensitivity of these results toour choice of queries (6fti N and 6itc C), selected abovefor maximum symmetry, we repeated them with the op-posite half of each structure

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