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17 Jun 2005: ease of vesicles that were not primed at the time theeptide was introduced.To test whether a population of vesicles were primed. ... irst stimulus (upper), the percentage of excess membrane re-rieved during the fast phase (middle), and the rate constant

24 Jan 2005: Theinner M2 helices also change their configuration inresponse to ACh, widening the lumen of the pore atthe middle of the membrane. ... The model-derivedphases provided a more sensitive test of the signalretained in the images at high resolution than

30 Jun 2004: parS. sequence5. ¢. -TGTTCCACGTGAAACA-3. ¢. (Fig. 1A and B). To test forthe ability of wild-type and truncated proteins to carry outDNA binding and spreading, we used an agarose gel ... Groups of 12 scans were taken shortly after theboundary cleared

28 Sep 2007: later). Following the middle domain, a40-residue linker connects the helical domain with the C-terminalEH-domain (residues 443–543). ... S, supernatant; P, pelleted fraction.d, Electron micrographs of negatively stainedPtdSer liposomes in the absence

22 Mar 2005: EpsinR was localized to endosomal cells that were recognized via thick tubular elements inmembranes, and involved in the retrograde transport of their cytoplasm (Figure 1G, middle). ... To test whether AP1 is required for the retro- dress its function in

20 Nov 2010: The fusion pathway consists of. membrane dimpling leading to the generation of curvature stresses and the establishment of point-like intermembrane contacts (left panel), fusion stalk formation (middle. ... Science 321, 1354–1357. 62 Sever, S. et al.

9 Nov 2001: Testing them will involve addressing ques-tions such as the interactions that localize T-tubules tothe middle of the M–Z interval, the binding partners ofthe SH3 domain of Drosophila amphiphysin ... For complementation tests, amph26 homozygoteswere

15 Mar 2018: thetic complex. To test this hypothesis, we examined MreB motions as we changed the shape of. ... increase with cell width (Ouzounov et al., 2016). To test if the angle of MreB movement changes.

21 Feb 2011: middle of the stalk and contributes similarly to theenergies of both fusion and fission stalks [3,73].[(Box_1)TD$FIG]. ... Science 321, 1354–1357. 62 Sever, S. et al. (2000) Garrotes, springs, ratchets, and whips: puttingdynamin models to the test.

17 Mar 2003: of polymerized clathrin triskelia making a lattice of hexa-gons and pentagons, a middle layer where clathrin and To whom correspondence should be addressed (e-mail: hmm@. ... In order to test the validity of this hypoth-by six residues to complete the b

19 Dec 2016: helical 2D class average (top), re-projections of F-actin double-stranded filament structure (middle) (von der Ecken et al., 2015) and crenactin single. ... crenactin filaments. To test this, we performed. the same experiment using a peptide spanning.

3 Sep 2008: Second, a molecule from the A layer of one. strand uses its a1/a2 loop to contact the middle of helix a2 of. ... some uncertainty of the molecular interpretation of the filaments,. so we carried out site-specific mutagenesis to test the contacts.

11 Aug 2015: To test this directly, we used nondenaturing affinity purification to assess a possible 60S-listerin-translocon ternary complex (Figure 1E). ... NCtRNA. NC. 87654321. 87654321. 654321. (middle) E1 and E2 were analyzed by SDS–PAGE and autoradiography.

7 Jul 1999: The middle sphere lies between the side-chains of Asp2 of one molecule in the asymmetricunit and a symmetry-related copy of the other mol-ecule in the asymmetric unit. ... The residuals betweenthe tted absorbance and the experimental values wereplotted,

13 Jun 2009: an in vivo interaction assay based on coassociation of an. FP-tagged test protein with FP-tagged cytosolic PrP aggregates. ... involving excessive PrP exposure to the cytosol. To test this. idea, we analyzed the status of Mgrn and lysosomes in trans-.

28 Nov 2008: The error bars show 95% confidence intervals (calculated by t tests) for each condition. ... top), internalized anti-GFP (middle), and a merged, triple-labeled image (bottom). Arrows indicate points of colocalization.

23 Oct 2012: parenthesis. † Rmerge = ΣΣ | Ihl - 〈Ih〉 | / ΣΣ 〈Ih〉 h l h l ‡ 5 % of the reflections were included in the test set. ... The middle segments (initial seed) of the filaments become photobleached as the movie progresses, while the intensity

9 Sep 2015: Comparison of sensitive and resistant TMDs at multiple points along the integration pathway (i.e., at multiple nascent chain lengths) should allow us to: (1) test whether they pass through a

7 Apr 2010: As a result, a membranefragment with an amphipathic helix in the middle has an effectivefunnel-like shape, which can fit to a sphere with a certain radiusand the corresponding total curvature.

4 Aug 2017: middle) or physical interactions (bottom). ... Fig. 2. Reconstitution ofUBE2O-mediated clientubiquitination with purifiedfactors. (A) Experimentalstrategy to test UBE2O inter-action and ubiquitination in adefined system.

22 Jul 2019: by MinD’s MTS. To test whether P. aeruginosa MinC and MinD pro-. ... pole oscillation of a protein required for directing. division to the middle of Escherichia coli.

18 Sep 2008: In this study, we have. performed experiments to test these predictions. ... After crosslinking, the products were fractionated into membrane-associated and. lumenal proteins, shown in the left and middle panels, respectively.

12 Oct 2002: Within the p47phox-PX PtdIns(3,4)P2-binding site,Arg43 [the middle residue of the conserved (R/K)-(R/K)-(F/Y) motif located in the b3/a1 loop] ... To test this notion, wemeasured the binding of mutants to vesicles contain-ing

28 Sep 2012: If you set it, Coot will test each ’:’-separatedstring that it points to a directory, and if it does, Coot will load all the. ... Rotate View around Screen Z axis. Middle-mouse. Centre on atom. Scroll-wheel Forward.

11 Jun 2013: Twinning tests summary. Space group validaAon. 04/06/2013 58 Crystallography Course LMB. ... Twinning tests summary. Space group validaAon. 04/06/2013 61 Crystallography Course LMB.

27 Jan 2018: MEMBRANE TARGETING. The ER membrane protein complex isa transmembrane domain insertaseAlina Guna,1 Norbert Volkmar,2 John C. Christianson,2 Ramanujan S. Hegde1†. Insertion of proteins into membranes is an essential cellular process. The

19 Mar 2013: 3C). To further test for auto-inhibition, we per-formed a series of co-sedimentation assays with SUVs (Fig. ... 42). Although our assay does notallow us to test for possible scission mechanisms, we can ask thefollowing question.

17 Mar 2003: test set of reflections, consisting of a randomly selected 5% of the data and not used during refinement. ... The segments (colored yellow) at the C-terminal end of a7 helix and the middle of a8 helix are engaged in an intramolecularantiparallel leucine

22 Aug 2007: GTP domain, middle domain, GED, PH, PRD, no clathrin binding sites, maybe adaptor binding via a few motifs. ... no addit. Dynamins (excluding Dynamin like and mitofusins). XP_001183998, similar to Dynamin 2 GTP domain, middle domain, PH, GED, but no PRD,

8 Dec 2007: Leu 64Vps4 and Glu 68Vps4 form key interactions withArg 224Vps2 in the middle of aC (Fig. ... To test whether MIMs have a role in sorting, we mutated motifs inboth Vps2 and Did2.

18 Dec 2008: 1C, middle panel). Immunostain-ing with antibodies to Saci1373 (ESCRT-III). revealed a distinct subcellular localization ofthe protein. ... To further test the invivo role of the Sulfolobus ESCRTcomponents, wegenerated an episomal vector to direct

16 May 2013: To test this idea, we produced RNCs ina ubiquitination-deficient lysate (Hessa et al., 2011) depleted ofthe requisite E2s and ubiquitin (Figure 4C). ... This suggests that their combinationprovides the specificity for Listerin recruitment. To test

14 Dec 2010: Toledo, Ohio, in the middle of winter and, finally, to San Francisco. ... After the. UK was a stop in Toledo, Ohio, in the middle of winter, not my top choice of places to.

20 Jul 2005: test this hypothesis, we analysed b5-Nglyc translocation. into protein-free liposomes of differing composition.

9 Nov 2011: The apparentfree-energy contribution of each amino acidin any test sequence was found to be additive,with the sum determining the final insertionprobability. ... earlier transmembrane domains ( gray), and a later test sequence(red ) is measured for

24 Aug 2011: A single section of the original tomogram is shown (left). Various rotations of a 3D contoured electron density render were generated (middle). ... 6 F). To test the requirement for CLIC endocytosis during mi-gration, this inactivation assay was applied

22 Jul 2008: We. analyzed subsets of the final data set to test this idea. ... low the tunnel exit (Figure 7, middle). Subsequent disassocia-. tion of the SRP-SR complex from the ribosome would precede.

21 Dec 2004: Differences among eachconstruct were statistically significant (p 0.01 by Student’s t test).(B) Ten nanograms per well of constructs encoding the indicatedsignal sequences fused to TF were analyzed for

15 Sep 2011: MOLECULAR BASIS FOR MEMBRANE REMODELLING  AND ORGANIZATION . Analyses moléculaires de l'organisation et du remodelage des membranes. President: Harvey McMAHON MRC Laboratory of Molecular Biology, Neurobiology Division,.

24 Jul 2020: To test this idea, we monitored translation output from a reporter mRNA containing a collision-. ... Hegde, 2017). This precluded a clear test of EDF1’s role using this stalling sequence.

14 Jul 2021: decreasing it with Pds5. To test this, we measured the effect of scc4-4 in the presence or absence of.

15 Dec 2021: To test the sensitivity of these results toour choice of queries (6fti N and 6itc C), selected abovefor maximum symmetry, we repeated them with the op-posite half of each structure