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The patterned assembly and stepwise Vps4-mediated disassembly of…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Hurtig%202023%20sciadv.ade5224.pdf16 Jul 2023: S1E). As a further test of thisfinding, we expressed the Vps4E209Q plasmid in a cell line carryinga thymidine kinase homolog (hereafter called STK). ... 3A).To test the functional significance of this patterned assembly ofESCRT-III polymers, we used -
Ribosome-associated protein quality control
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/98_Brandman_O_NSMB_2016.pdf18 Feb 2016: Thus, the idea emerged that mRNAs are ‘tested’ for their integrity by translation, and failure of this test initiates their degradation to prevent production of faulty proteins1,31. ... An advantage of encoding an arrest-inducing sequence in the -
doi:10.1016/j.cell.2006.03.035
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/16647110.pdf14 Jun 2006: Middle) Plot of the similarity between PI3-Ks according to their IC50 values against small molecule. ... cell culture are recapitulated in vivo by performing insulin. tolerance tests in mice. -
DOI: 10.1126/science.1256917, 963 (2014);346 Science et al.Thomas G.…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Science-2014-Gligoris-963-7.pdf21 Nov 2014: Scc1 contains many more residues in the middle domain. Separase cleavage sites, Pds5(7) and Scc3 binding sites are highlighted (28). ... S7E). Sister DNAs are entrapped bycohesin trimers. To test whether cross-linking all three interfacesof the cohesin -
FtsA forms actin-like protofilaments
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/EMBO2012FtsAPiotr/emboj201276a.pdf27 Mar 2012: and Errington, 2009). FtsZ finds the middle of the cell. through two independent mechanisms in E. ... colicells and imaged by electron cryotomography. EcFtsA without the amphipathic helix (top left) forms long, straight bundles in the middle of thecell -
Structural analysis of the ParR/parC plasmidpartition complex Jakob…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MollerJensen_ParR_EMMBO_2007.pdf4 Oct 2007: tion. Mutant parR genes were tested for functionality in their. natural genetic context on test plasmids derived from low-. ... As shown in Figure 4A, test plasmids. containing the ParR171 K6E, R7S and K8E mutations severely. -
Volume 22 May 15, 2011 1625 Cytosolic aggregates perturb ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/69_Chakrabarti_O_MBC_2011.pdf11 Nov 2011: The aggregated protein indicated at left was coexpressed with the test protein indicated along the top. ... At each time point, the % of aggregate-containing cells that showed excess intracellular accumulation of the test protein is indicated. -
Mechanism of endophilin N-BAR domain-mediated membrane curvature…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndophilinEMBO/GallopEndophilin2006.pdf8 Jun 2006: implying the formation of additional helical structures. To test. how the predicted N-terminal amphipathic helix folds,. ... seeResults). Student’s t-test Po0.001, Po0.2. Endophilin and vesicle buddingJL Gallop et al. & -
ppat.1000479 1..19
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/60_Ashok_A_PLoS_Path_2009.pdf20 Jun 2009: Horizontal black barsindicate the mean values for each data set. Each mutant dataset was compared to wtPrP by the Student’s t-test and found to be statisticallysignificant in all cases -
JCB Article The Journal of Cell Biology, Volume 164, ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/28_Snapp_EL_JCB_2004.pdf15 Apr 2004: The left and middle panels used directly conjugated anti-CNX antibodies against the COOH and NH2 terminus, respectively. ... multiple days.aTreatment is significant (P 0.01) compared with untreated cells using t test.
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