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LMB 365 - Day 149 - MRC Laboratory of Molecular Biology
https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-149%2F&format=xmld.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... The supporting metal helices in the middle of the stem have some parts where the nucleotide bases are left out, -
Phil. Trans. Roy. Soc. Lond. B. 261, 95-104 (1971) ...
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ptrs71.pdf26 Nov 2012: electron diffraction pattern: for the weakly scattering material we wish to examine, the high intensity of the central order beam would ensure that the potential at the middle of the thread ... The requirement now is that the potential at the middle of -
Bacterial actin MreB assembles in complex with cell shape protein RodZ
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embo2010RodZMreB.pdf17 Feb 2010: B) MreB polymerisation assays wereused to test the interface mutants of RodZ for MreB binding. ... structures from whichfilaments grow when mixed together in the presence of nucleotide and MgCl2 (middle panel). -
61 Ultramicroscopy 25 (1988) 279-292 North-HoUand, Amsterdam 279…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ultram88.pdf1 Apr 2016: In this paper, we examine the behaviour of the CTF for thin ice-embedded specimens within the framework of the hnear theory of image forma-tion, using as a test object ... correspond to 0.1 fim (left and middle) and 1/50 A " ' (right). -
2623.tif
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb90.pdf23 Jan 2012: Sections at the horizontal line near the middle of the tube are shown in Fig. ... The ridges are quite prominent near the membrane but broad and smooth near the synaptic end; surface features are more complicated in the middle region. -
Insight into bacterial cell division: Architecture of the FtsZ ring - …
https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Finsight-bacterial-cell-division-architecture-ftsz-ring%2F&format=xmlt.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... n=new -
MinCD cell division proteins form alternating copolymeric cytomotive…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/ncomms6341.pdf6 Dec 2014: 1a,d). MinC and MinD copolymerize to form an alternatingcopolymer. To experimentally test filament formation, we usedfull-length, untagged E. ... A. Rapid pole-to-pole oscillation of a proteinrequired for directing division to the middle of Escherichia -
Coot Scripting Interface: cc-interface.hh Source File
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/docs/html/cc-interface_8hh_source.html24 Aug 2021: 615 // test the returned spec for unset_p(). -
PNAS202120006_proof
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/pnas%20Lee%202022.pdf18 Dec 2022: To test thesehypotheses, we used the same N-terminal Brn1 release assay(Fig. ... 4. (A, Left and Middle) Condensin pentamer clamping circular plasmid DNA, as observed by cryo-EM with a VPP. -
Membrane transport between compartments in eukary-otic cells requires …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Dynaminreview2004.pdf2 Feb 2004: Sequence homology does not allow us todefine the region between the middle domain and the. ... In humanMxA, a single point mutation (L612K) in the GEDblocks the interaction with the middle domain andinhibits oligomerization74. -
The structure of bactofilin filaments reveals their mode of membrane…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Deng2019Natmicro.pdf26 Aug 2019: Left, magnifications of the corners of a. Middle, magnifications of the corners of b with the TtBac sequence added beneath. ... coli. N. N. N. N. WT. Liposome. BactofilinBactofilin. Liposome. WT: middle of cell WT: top of cell. -
Thesis Chapter 0
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/Hardaker.pdf10 Jun 2008: To test the possible involvement of FLP-1-encoded peptides in the modulation of egg-. -
Qrb1300006 1..40
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/qrb13.pdf30 Jul 2013: twist around the tube axis). Preliminary tests using a segment size similar to that in Fig. ... 7b). As will be. described in Section 4.3, the gate of the channel is located near the middle of the membrane-. -
30 Apr 2004 18:9 AR AR214-BB33-09.tex AR214-BB33-09.sgm…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/annrev2004.pdf1 May 2004: FtsZ is the organizingmolecule of bacterial cell division and forms a filamentous ring around the middle ofthe cell. ... At least theparC/ParR/M system is simple enoughto test this possibility in vitro. -
doi:10.1016/j.molcel.2008.05.027
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsK%20MolCell%202008%20Lowe.pdf16 Aug 2008: generally further away from the DNA with many water molecules. mediating contacts with the DNA (Figures 1E, middle, S1D, and. ... sequently activate recombination. In order to test whether the dimeric forms of the protein had. -
PII: S1097-2765(02)00515-4
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/SMC%20cohesin%20paper%202002.pdf19 Apr 2002: Upper two rows, open V conformation; middle two rows, Yconformation; lower row, coils spread conformation. ... To test this directly, we repeated the aboveexperiment using a yeast strain expressing Scc1myc18Cohesin Contains Only a Single Molecule. -
Identifying proteins bound to native mitotic ESC chromosomes reveals…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Djeghloul%20Nat%20Camms%202020.pdf11 Aug 2020: P-values of statistically significant increases, measured by unpaired two tailed Student’s t-tests, are indicated. ... P-values of statisticallysignificant decreases, measured by unpaired two tailed Student’s t-tests, are indicated. -
pone.0107211 1..10
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/leoa2014.pdf3 Sep 2014: tween the classical dynamin middle domain and GTPase effector. domain (GED) (Figure 1B). ... per other DFMs) a predicted membrane binding domain between. a middle domain and a GTPase effector domain (GED). -
53091 361..366
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/McMahonE.pdf3 Dec 2004: endogenous AP2 (middle, AP6 antibody) and transferrin uptake (right). A strong. ... Liposome tubulation, sedimentation and electron microscopySynthetic liposomes contained 10% cholesterol, 40% phosphatidylethanolamine,40% phosphatidylcholine, and 10% of -
1 Supplementary data to accompany paper: Mechanism of endophilin ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndophilinEMBO/EndoSupMethods.pdf8 Jun 2006: simple gaussian functions to dc/dt versus s curves to test for the number of components and. ... amphipathic helix are disordered, as well as a loop in the middle of the first helix which. -
A-nsmb855.indd
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nsmb%20FtsZ%202004.pdf19 Nov 2004: 2). The break in the protofilament is necessary for crystallization because the dimer formed by the 43-Å interaction (middle of Fig. ... To test for bound nucleotide, FtsZ was precipitated using 0.5 N cold HClO4, and after centrifugation nucleotide -
Molecular Biology of the CellVol. 8, 2003–2015, October 1997 ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/WiggeKohler_McMahon1997.pdf10 Oct 1997: To test this directly, Amph2 immunoprecipi-tates were Coomassie-stained, revealing that Amph1. ... This provided us with a useful tool to test theimportance of the heterodimer in vivo. -
doi:10.1016/j.cell.2006.01.047
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/16615893.pdf18 Apr 2006: through an adaptor protein. To test whether these two. complexes can directly interact in vitro, we used analyt-. ... Avanti or Echelon). Control vesicles contained an additional 10% PC. instead of the test lipid. -
doi:10.1016/j.str.2007.05.002
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/FCH02F-BARHenne07.pdf2 Jun 2007: protein saturation on liposomes. To test more directly whether the dimer seen in the crys-. ... tutive helix. To test for a role for helix 1 in membrane bind-. -
Twinning and other pathologies Andrey Lebedev CCP4 OD-‐structures…
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/%22https:/www2.mrc-lmb.cam.ac.uk/download/twinningaandaotherapathologiesa-andreyalebedeva-a/?wpdmdl=18788&11 Jun 2013: Twinning tests summary. Space group validaAon. 04/06/2013 58 Crystallography Course LMB. ... Twinning tests summary. Space group validaAon. 04/06/2013 61 Crystallography Course LMB. -
A positively charged channel within the Smc1/Smc3 hinge required for…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embosmcjingekurze2010.pdf1 Dec 2010: form a pseudo twofold symmetric torus with a small channel. in the middle. ... generate sister chromatid cohesion. To test the effect of charge neutralization on cohesin complex. -
doi:10.1016/j.molcel.2004.08.030
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Haering%20Mol%20Cell%202004.pdf20 Sep 2004: However, the disorder makes it difficult todesign specific mutants to test this hypothesis.its C terminus (Figure 1D). ... To test whether the S525N mutation Is There Any Turnover of Cohesin Subunits. -
elife.elifesciences.org MacKinnon et al. eLife 2014;3:e01483. DOI:…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/83_MacKinnon_AL_eLife_2014.pdf9 Sep 2015: Comparison of sensitive and resistant TMDs at multiple points along the integration pathway (i.e., at multiple nascent chain lengths) should allow us to: (1) test whether they pass through a -
cdf519 5057..5068
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/12356722.pdf12 Oct 2002: Within the p47phox-PX PtdIns(3,4)P2-binding site,Arg43 [the middle residue of the conserved (R/K)-(R/K)-(F/Y) motif located in the b3/a1 loop] ... To test this notion, wemeasured the binding of mutants to vesicles contain-ing -
JFCregg_OGS_finaledit
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/Cregg_thesis.pdf14 Dec 2010: Toledo, Ohio, in the middle of winter and, finally, to San Francisco. ... After the. UK was a stop in Toledo, Ohio, in the middle of winter, not my top choice of places to. -
Cryo‐EM structure of the MinCD copolymeric filament from Pseudomonas…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Szewczak-Harris_et_al-2019-FEBS_Letters.pdf22 Jul 2019: by MinD’s MTS. To test whether P. aeruginosa MinC and MinD pro-. ... pole oscillation of a protein required for directing. division to the middle of Escherichia coli. -
x16412u141
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/MaoAP180-2001.pdf17 Mar 2003: test set of reflections, consisting of a randomly selected 5% of the data and not used during refinement. ... The segments (colored yellow) at the C-terminal end of a7 helix and the middle of a8 helix are engaged in an intramolecularantiparallel leucine -
doi:10.1016/j.devcel.2008.06.015
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/54_Rane_NS_DevCell_2008.pdf18 Sep 2008: In this study, we have. performed experiments to test these predictions. ... After crosslinking, the products were fractionated into membrane-associated and. lumenal proteins, shown in the left and middle panels, respectively. -
Supplementary Text & Figs
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Schmid_Nature2007Sup.pdf22 Aug 2007: GTP domain, middle domain, GED, PH, PRD, no clathrin binding sites, maybe adaptor binding via a few motifs. ... no addit. Dynamins (excluding Dynamin like and mitofusins). XP_001183998, similar to Dynamin 2 GTP domain, middle domain, PH, GED, but no PRD, -
Twinning and other pathologies Andrey Lebedev CCP4 OD-‐structures…
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_crystallography_course_2013/lectures/pathologies_and_twinning.pdf11 Jun 2013: Twinning tests summary. Space group validaAon. 04/06/2013 58 Crystallography Course LMB. ... Twinning tests summary. Space group validaAon. 04/06/2013 61 Crystallography Course LMB. -
coot-user-manual.dvi
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/docs/coot-user-manual.pdf28 Sep 2012: If you set it, Coot will test each ’:’-separatedstring that it points to a directory, and if it does, Coot will load all the. ... Rotate View around Screen Z axis. Middle-mouse. Centre on atom. Scroll-wheel Forward. -
Cooperative Recruitment of Dynamin and BIN/Amphiphysin/Rvs (BAR)…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/JBC_Meinecke2013.pdf19 Mar 2013: 3C). To further test for auto-inhibition, we per-formed a series of co-sedimentation assays with SUVs (Fig. ... 42). Although our assay does notallow us to test for possible scission mechanisms, we can ask thefollowing question. -
ProSHADE: src/proshade/ProSHADE_saveLoad.cpp Source File
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/d0/df1/_pro_s_h_a_d_e__save_load_8cpp_source.html1 Nov 2018: ProSHADE 0.6.5 (NOV 2018). Protein Shape Descriptors and Symmetry Detection. ProSHADE_saveLoad.cpp. Go to the documentation of this file.. 1. 21 //============================================ RVAPI. 22 #include <rvapi_interface.h>. 23. 24 //========= -
Supplement_Final
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/81_Shao_S_Mol_Cell_2013.pdf16 May 2013: To test this idea, we produced RNCs ina ubiquitination-deficient lysate (Hessa et al., 2011) depleted ofthe requisite E2s and ubiquitin (Figure 4C). ... This suggests that their combinationprovides the specificity for Listerin recruitment. To test -
Abstract_Book_HMM_v2
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/conferences/JM_Abstract_Book.pdf15 Sep 2011: MOLECULAR BASIS FOR MEMBRANE REMODELLING AND ORGANIZATION . Analyses moléculaires de l'organisation et du remodelage des membranes. President: Harvey McMAHON MRC Laboratory of Molecular Biology, Neurobiology Division,. -
Membrane Protein Insertion at the Endoplasmic Reticulum
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/75_Shao_S_Ann_Rev_2011.pdf9 Nov 2011: The apparentfree-energy contribution of each amino acidin any test sequence was found to be additive,with the sum determining the final insertionprobability. ... earlier transmembrane domains ( gray), and a later test sequence(red ) is measured for -
Transmembrane topogenesis of a tail-anchoredprotein is modulated by…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/36_Brambillasca_S_EMBO_2005.pdf20 Jul 2005: test this hypothesis, we analysed b5-Nglyc translocation. into protein-free liposomes of differing composition. -
Molecular Biology of the CellVol. 16, 279 –291, January ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/32_Levine_CG_MBC_2005.pdf21 Dec 2004: Differences among eachconstruct were statistically significant (p 0.01 by Student’s t test).(B) Ten nanograms per well of constructs encoding the indicatedsignal sequences fused to TF were analyzed for -
16262551036386 1..32
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/cohesin%20folding%202021%20elife-67268-v1.pdf14 Jul 2021: decreasing it with Pds5. To test this, we measured the effect of scc4-4 in the presence or absence of. -
JCB: Article The Rockefeller University Press $30.00J. Cell Biol. ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/J%20Cell%20Biol%202010%20Howes.pdf24 Aug 2011: A single section of the original tomogram is shown (left). Various rotations of a 3D contoured electron density render were generated (middle). ... 6 F). To test the requirement for CLIC endocytosis during mi-gration, this inactivation assay was applied -
doi:10.1016/j.str.2008.05.003
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/52_Menetret_JF_Structure_2008.pdf22 Jul 2008: We. analyzed subsets of the final data set to test this idea. ... low the tunnel exit (Figure 7, middle). Subsequent disassocia-. tion of the SRP-SR complex from the ribosome would precede. -
15955888524538 1..29
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2020/09/eLife_2020b.pdf24 Jul 2020: To test this idea, we monitored translation output from a reporter mRNA containing a collision-. ... Hegde, 2017). This precluded a clear test of EDF1’s role using this stalling sequence. -
A unified evolutionary origin for the ubiquitous protein transporters …
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2023/08/BMC_Biology_2021.pdf15 Dec 2021: To test the sensitivity of these results toour choice of queries (6fti N and 6itc C), selected abovefor maximum symmetry, we repeated them with the op-posite half of each structure
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