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  2. KWRM_A_992668_O

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-3.pdf
    20 Oct 2015: 3A, middle).We were thus able to introduce a qualita-tive modification to the C. ... We inserted an electrical syn-apse between AWC and AIY (Fig. 3B,middle).
  3. Impress

    https://www2.mrc-lmb.cam.ac.uk/coot/ephemera/beyond-coloured-lines.pdf
    28 Sep 2020: see the video– Ctrl <middle-mouse> scroll. 4 / 50. “3D sticks in space” is the way in which Coot 0.0-0.9.1 has represented models and maps. ... 28 / 50. Atoms Clashing. Test-bed/Hello World. Ribosomes at 60FPS. Ribosomes at 60FPS.
  4. Using Coot for model building into EM maps:A tutorial ...

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/EM-Tutorial-Coot-PE.pdf
    8 Sep 2015: The test case for the tutorial is F420-reducing NiFe hydrogenase (Frh) {Allegretti:2014tz}. ... As a test case, we are going to use chain A from Frh.
  5. MeganPalm

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/MeganPalm.pdf
    3 Dec 2005: previously used for the egg detection test and 20 new normal wild type videos. ... in 5 sec CANGCRMAX Max angle change rate in middle sect.TLMV10AVG Avg.
  6. supplementary figures 070814 JL

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/scc32014si.pdf
    8 Aug 2014: and Zr Scc3. (A) Zr Scc3 structure, same as Fig. 1A, middle, colored blue to red from N- to C-. ... flow through, b: bound). (B) To test if Sc Scc3 forms homodimers, full length.
  7. Membrane Localization is Critical for Activation of the PICK1 BAR…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/MadsenGetherTraffic2008.pdf
    18 Oct 2008: eYFP–PICK1 wt (middle row) or to-. gether with eYFP–PICK1 3KE (bottom. ... green (middle column). Right column. shows the merged pictures. White. bar 10 mm.
  8. Features critical for membrane binding revealed by DivIVA crystal…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/emboj201099a.pdf
    20 May 2010: membrane interaction. To test this, we purified the F17A. mutant and investigated membrane binding in vitro. ... could conveniently test suppressors for restored localization. The motivation for such screen was that suppressors of the.
  9. Dissertation

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/AndyHuang.pdf
    6 Mar 2008: 74. Table 4.5: The results of the significance test (p-value < 0.05 is considered to be significant).
  10. Influence of lipid bilayer on the structure of the muscle-­type…

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnasS24.pdf
    30 Apr 2024: 1 A, Middle) and a symmetrical rim of MX helices lying flat against the inner (cytoplasmic) bilayer surface (Fig. ... B) In-plane slabs through densities and superimposed model cutting through the top, middle and bottom portions of the TM helices.
  11. Twinning and other pathologies Andrey Lebedev CCP4 OD-­‐structures…

    https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_crystallography_course_2013/lectures/pathologies_and_twinning.pdf
    11 Jun 2013: Twinning tests summary. Space group validaAon. 04/06/2013 58 Crystallography Course LMB. ... Twinning tests summary. Space group validaAon. 04/06/2013 61 Crystallography Course LMB.
  12. Twinning and other pathologies Andrey Lebedev CCP4 OD-­‐structures…

    https://www2.mrc-lmb.cam.ac.uk/download/twinningaandaotherapathologiesa-andreyalebedeva-a/?wpdmdl=18788&refresh=668aac7b788ee1720364155
    11 Jun 2013: Twinning tests summary. Space group validaAon. 04/06/2013 58 Crystallography Course LMB. ... Twinning tests summary. Space group validaAon. 04/06/2013 61 Crystallography Course LMB.
  13. Phil. Trans. Roy. Soc. Lond. B. 261, 95-104 (1971) ...

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ptrs71.pdf
    26 Nov 2012: electron diffraction pattern: for the weakly scattering material we wish to examine, the high intensity of the central order beam would ensure that the potential at the middle of the thread ... The requirement now is that the potential at the middle of
  14. Bacterial actin MreB assembles in complex with cell shape protein RodZ

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embo2010RodZMreB.pdf
    17 Feb 2010: B) MreB polymerisation assays wereused to test the interface mutants of RodZ for MreB binding. ... structures from whichfilaments grow when mixed together in the presence of nucleotide and MgCl2 (middle panel).
  15. 61 Ultramicroscopy 25 (1988) 279-292 North-HoUand, Amsterdam 279…

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ultram88.pdf
    1 Apr 2016: In this paper, we examine the behaviour of the CTF for thin ice-embedded specimens within the framework of the hnear theory of image forma-tion, using as a test object ... correspond to 0.1 fim (left and middle) and 1/50 A " ' (right).
  16. 2623.tif

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb90.pdf
    23 Jan 2012: Sections at the horizontal line near the middle of the tube are shown in Fig. ... The ridges are quite prominent near the membrane but broad and smooth near the synaptic end; surface features are more complicated in the middle region.
  17. Membrane transport between compartments in eukary-otic cells requires …

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Dynaminreview2004.pdf
    2 Feb 2004: Sequence homology does not allow us todefine the region between the middle domain and the. ... In humanMxA, a single point mutation (L612K) in the GEDblocks the interaction with the middle domain andinhibits oligomerization74.
  18. 1 Supplementary data to accompany paper: Mechanism of endophilin ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndophilinEMBO/EndoSupMethods.pdf
    8 Jun 2006: simple gaussian functions to dc/dt versus s curves to test for the number of components and. ... amphipathic helix are disordered, as well as a loop in the middle of the first helix which.
  19. MinCD cell division proteins form alternating copolymeric cytomotive…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/ncomms6341.pdf
    6 Dec 2014: 1a,d). MinC and MinD copolymerize to form an alternatingcopolymer. To experimentally test filament formation, we usedfull-length, untagged E. ... A. Rapid pole-to-pole oscillation of a proteinrequired for directing division to the middle of Escherichia
  20. doi:10.1016/j.molcel.2008.05.027

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsK%20MolCell%202008%20Lowe.pdf
    16 Aug 2008: generally further away from the DNA with many water molecules. mediating contacts with the DNA (Figures 1E, middle, S1D, and. ... sequently activate recombination. In order to test whether the dimeric forms of the protein had.
  21. PNAS202120006_proof

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/pnas%20Lee%202022.pdf
    18 Dec 2022: To test thesehypotheses, we used the same N-terminal Brn1 release assay(Fig. ... 4. (A, Left and Middle) Condensin pentamer clamping circular plasmid DNA, as observed by cryo-EM with a VPP.
  22. PII: S1097-2765(02)00515-4

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/SMC%20cohesin%20paper%202002.pdf
    19 Apr 2002: Upper two rows, open V conformation; middle two rows, Yconformation; lower row, coils spread conformation. ... To test this directly, we repeated the aboveexperiment using a yeast strain expressing Scc1myc18Cohesin Contains Only a Single Molecule.
  23. The structure of bactofilin filaments reveals their mode of membrane…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Deng2019Natmicro.pdf
    26 Aug 2019: Left, magnifications of the corners of a. Middle, magnifications of the corners of b with the TtBac sequence added beneath. ... coli. N. N. N. N. WT. Liposome. BactofilinBactofilin. Liposome. WT: middle of cell WT: top of cell.
  24. Thesis Chapter 0

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/Hardaker.pdf
    10 Jun 2008: To test the possible involvement of FLP-1-encoded peptides in the modulation of egg-.
  25. Qrb1300006 1..40

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/qrb13.pdf
    30 Jul 2013: twist around the tube axis). Preliminary tests using a segment size similar to that in Fig. ... 7b). As will be. described in Section 4.3, the gate of the channel is located near the middle of the membrane-.
  26. 30 Apr 2004 18:9 AR AR214-BB33-09.tex AR214-BB33-09.sgm…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/annrev2004.pdf
    1 May 2004: FtsZ is the organizingmolecule of bacterial cell division and forms a filamentous ring around the middle ofthe cell. ... At least theparC/ParR/M system is simple enoughto test this possibility in vitro.
  27. Identifying proteins bound to native mitotic ESC chromosomes reveals…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Djeghloul%20Nat%20Camms%202020.pdf
    11 Aug 2020: P-values of statistically significant increases, measured by unpaired two tailed Student’s t-tests, are indicated. ... P-values of statisticallysignificant decreases, measured by unpaired two tailed Student’s t-tests, are indicated.
  28. doi:10.1016/j.cell.2006.01.047

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/16615893.pdf
    18 Apr 2006: through an adaptor protein. To test whether these two. complexes can directly interact in vitro, we used analyt-. ... Avanti or Echelon). Control vesicles contained an additional 10% PC. instead of the test lipid.
  29. Molecular Biology of the CellVol. 8, 2003–2015, October 1997 ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/WiggeKohler_McMahon1997.pdf
    10 Oct 1997: To test this directly, Amph2 immunoprecipi-tates were Coomassie-stained, revealing that Amph1. ... This provided us with a useful tool to test theimportance of the heterodimer in vivo.
  30. 53091 361..366

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/McMahonE.pdf
    3 Dec 2004: endogenous AP2 (middle, AP6 antibody) and transferrin uptake (right). A strong. ... Liposome tubulation, sedimentation and electron microscopySynthetic liposomes contained 10% cholesterol, 40% phosphatidylethanolamine,40% phosphatidylcholine, and 10% of
  31. A-nsmb855.indd

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nsmb%20FtsZ%202004.pdf
    19 Nov 2004: 2). The break in the protofilament is necessary for crystallization because the dimer formed by the 43-Å interaction (middle of Fig. ... To test for bound nucleotide, FtsZ was precipitated using 0.5 N cold HClO4, and after centrifugation nucleotide
  32. pone.0107211 1..10

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/leoa2014.pdf
    3 Sep 2014: tween the classical dynamin middle domain and GTPase effector. domain (GED) (Figure 1B). ... per other DFMs) a predicted membrane binding domain between. a middle domain and a GTPase effector domain (GED).
  33. A positively charged channel within the Smc1/Smc3 hinge required for…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embosmcjingekurze2010.pdf
    1 Dec 2010: form a pseudo twofold symmetric torus with a small channel. in the middle. ... generate sister chromatid cohesion. To test the effect of charge neutralization on cohesin complex.
  34. doi:10.1016/j.molcel.2004.08.030

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Haering%20Mol%20Cell%202004.pdf
    20 Sep 2004: However, the disorder makes it difficult todesign specific mutants to test this hypothesis.its C terminus (Figure 1D). ... To test whether the S525N mutation Is There Any Turnover of Cohesin Subunits.
  35. doi:10.1016/j.str.2007.05.002

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/FCH02F-BARHenne07.pdf
    2 Jun 2007: protein saturation on liposomes. To test more directly whether the dimer seen in the crys-. ... tutive helix. To test for a role for helix 1 in membrane bind-.
  36. Cooperative Recruitment of Dynamin and BIN/Amphiphysin/Rvs (BAR)…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/JBC_Meinecke2013.pdf
    19 Mar 2013: 3C). To further test for auto-inhibition, we per-formed a series of co-sedimentation assays with SUVs (Fig. ... 42). Although our assay does notallow us to test for possible scission mechanisms, we can ask thefollowing question.
  37. 53091 361..366

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/McMahon01020.pdf
    3 Dec 2004: endogenous AP2 (middle, AP6 antibody) and transferrin uptake (right). A strong. ... Liposome tubulation, sedimentation and electron microscopySynthetic liposomes contained 10% cholesterol, 40% phosphatidylethanolamine,40% phosphatidylcholine, and 10% of
  38. JFCregg_OGS_finaledit

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/Cregg_thesis.pdf
    14 Dec 2010: Toledo, Ohio, in the middle of winter and, finally, to San Francisco. ... After the. UK was a stop in Toledo, Ohio, in the middle of winter, not my top choice of places to.
  39. The Hydrophobic Insertion Mechanism of Membrane Curvature Generation…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/CampeloBioPJ2008.pdf
    13 Dec 2008: inclusion reaching its middle (Fig. 4 b) will not result in themembrane bending since the upper monolayer can expand. ... 5). To test the sensitivity of the model. predictions to this assumption, we repeated the calculations.
  40. Tumour prevention by a single antibody domaintargeting the…

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17568777.pdf
    22 Jul 2007: F-actin stress fibres (Figure 2E, middle panel compared with. uninfected cells or cells infected with retrovirus expressing. ... In addition,. using mouse models as a preclinical test, as we have done in.
  41. PHAGOCYTES, GRANULOCYTES, AND MYELOPOIESIS PSTPIP2 deficiency in mice …

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Blood-2012-Chitu-3126-35.pdf
    12 Oct 2012: Imageswere obtained with a cooled EMCCD camera (9100-02; Hamamatsu). Statistics. Significance was tested using the 2-tailed Student t test. ... Pseudocolored fluorescence image ofCOS-7 cells expressing EGFP alone (left), WT EGFP-PSTPIP2 (middle) or
  42. The patterned assembly and stepwise Vps4-mediated disassembly of…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Hurtig%202023%20sciadv.ade5224.pdf
    16 Jul 2023: S1E). As a further test of thisfinding, we expressed the Vps4E209Q plasmid in a cell line carryinga thymidine kinase homolog (hereafter called STK). ... 3A).To test the functional significance of this patterned assembly ofESCRT-III polymers, we used
  43. Ribosome-associated protein quality control

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/98_Brandman_O_NSMB_2016.pdf
    18 Feb 2016: Thus, the idea emerged that mRNAs are ‘tested’ for their integrity by translation, and failure of this test initiates their degradation to prevent production of faulty proteins1,31. ... An advantage of encoding an arrest-inducing sequence in the
  44. doi:10.1016/j.cell.2006.03.035

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/16647110.pdf
    14 Jun 2006: Middle) Plot of the similarity between PI3-Ks according to their IC50 values against small molecule. ... cell culture are recapitulated in vivo by performing insulin. tolerance tests in mice.
  45. DOI: 10.1126/science.1256917, 963 (2014);346 Science et al.Thomas G.…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Science-2014-Gligoris-963-7.pdf
    21 Nov 2014: Scc1 contains many more residues in the middle domain. Separase cleavage sites, Pds5(7) and Scc3 binding sites are highlighted (28). ... S7E). Sister DNAs are entrapped bycohesin trimers. To test whether cross-linking all three interfacesof the cohesin
  46. FtsA forms actin-like protofilaments

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/EMBO2012FtsAPiotr/emboj201276a.pdf
    27 Mar 2012: and Errington, 2009). FtsZ finds the middle of the cell. through two independent mechanisms in E. ... colicells and imaged by electron cryotomography. EcFtsA without the amphipathic helix (top left) forms long, straight bundles in the middle of thecell
  47. Structural analysis of the ParR/parC plasmidpartition complex Jakob…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MollerJensen_ParR_EMMBO_2007.pdf
    4 Oct 2007: tion. Mutant parR genes were tested for functionality in their. natural genetic context on test plasmids derived from low-. ... As shown in Figure 4A, test plasmids. containing the ParR171 K6E, R7S and K8E mutations severely.
  48. Volume 22 May 15, 2011 1625 Cytosolic aggregates perturb ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/69_Chakrabarti_O_MBC_2011.pdf
    11 Nov 2011: The aggregated protein indicated at left was coexpressed with the test protein indicated along the top. ... At each time point, the % of aggregate-containing cells that showed excess intracellular accumulation of the test protein is indicated.
  49. Mechanism of endophilin N-BAR domain-mediated membrane curvature…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndophilinEMBO/GallopEndophilin2006.pdf
    8 Jun 2006: implying the formation of additional helical structures. To test. how the predicted N-terminal amphipathic helix folds,. ... seeResults). Student’s t-test Po0.001, Po0.2. Endophilin and vesicle buddingJL Gallop et al. &
  50. JCB Article The Journal of Cell Biology, Volume 164, ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/28_Snapp_EL_JCB_2004.pdf
    15 Apr 2004: The left and middle panels used directly conjugated anti-CNX antibodies against the COOH and NH2 terminus, respectively. ... multiple days.aTreatment is significant (P 0.01) compared with untreated cells using t test.
  51. ppat.1000479 1..19

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/60_Ashok_A_PLoS_Path_2009.pdf
    20 Jun 2009: Horizontal black barsindicate the mean values for each data set. Each mutant dataset was compared to wtPrP by the Student’s t-test and found to be statisticallysignificant in all cases

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