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  2. LETTERS Structural basis for selective recognition of ESCRT-IIIby the …

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17928861.pdf
    8 Dec 2007: Leu 64Vps4 and Glu 68Vps4 form key interactions withArg 224Vps2 in the middle of aC (Fig. ... To test whether MIMs have a role in sorting, we mutated motifs inboth Vps2 and Did2.
  3. Supplement_Final

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/81_Shao_S_Mol_Cell_2013.pdf
    16 May 2013: To test this idea, we produced RNCs ina ubiquitination-deficient lysate (Hessa et al., 2011) depleted ofthe requisite E2s and ubiquitin (Figure 4C). ... This suggests that their combinationprovides the specificity for Listerin recruitment. To test
  4. DOI: 10.1126/science.1165322 , 1710 (2008); 322Science et al.Rachel…

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/19008417.pdf
    18 Dec 2008: 1C, middle panel). Immunostain-ing with antibodies to Saci1373 (ESCRT-III). revealed a distinct subcellular localization ofthe protein. ... To further test the invivo role of the Sulfolobus ESCRTcomponents, wegenerated an episomal vector to direct
  5. Transmembrane topogenesis of a tail-anchoredprotein is modulated by…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/36_Brambillasca_S_EMBO_2005.pdf
    20 Jul 2005: test this hypothesis, we analysed b5-Nglyc translocation. into protein-free liposomes of differing composition.
  6. Membrane Protein Insertion at the Endoplasmic Reticulum

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/75_Shao_S_Ann_Rev_2011.pdf
    9 Nov 2011: The apparentfree-energy contribution of each amino acidin any test sequence was found to be additive,with the sum determining the final insertionprobability. ... earlier transmembrane domains ( gray), and a later test sequence(red ) is measured for
  7. JCB: Article The Rockefeller University Press $30.00J. Cell Biol. ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/J%20Cell%20Biol%202010%20Howes.pdf
    24 Aug 2011: A single section of the original tomogram is shown (left). Various rotations of a 3D contoured electron density render were generated (middle). ... 6 F). To test the requirement for CLIC endocytosis during mi-gration, this inactivation assay was applied
  8. doi:10.1016/j.str.2008.05.003

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/52_Menetret_JF_Structure_2008.pdf
    22 Jul 2008: We. analyzed subsets of the final data set to test this idea. ... low the tunnel exit (Figure 7, middle). Subsequent disassocia-. tion of the SRP-SR complex from the ribosome would precede.
  9. Molecular Biology of the CellVol. 16, 279 –291, January ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/32_Levine_CG_MBC_2005.pdf
    21 Dec 2004: Differences among eachconstruct were statistically significant (p 0.01 by Student’s t test).(B) Ten nanograms per well of constructs encoding the indicatedsignal sequences fused to TF were analyzed for
  10. Abstract_Book_HMM_v2

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/conferences/JM_Abstract_Book.pdf
    15 Sep 2011: MOLECULAR BASIS FOR MEMBRANE REMODELLING  AND ORGANIZATION . Analyses moléculaires de l'organisation et du remodelage des membranes. President: Harvey McMAHON MRC Laboratory of Molecular Biology, Neurobiology Division,.
  11. 15955888524538 1..29

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2020/09/eLife_2020b.pdf
    24 Jul 2020: To test this idea, we monitored translation output from a reporter mRNA containing a collision-. ... Hegde, 2017). This precluded a clear test of EDF1’s role using this stalling sequence.
  12. A unified evolutionary origin for the ubiquitous protein transporters …

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2023/08/BMC_Biology_2021.pdf
    15 Dec 2021: To test the sensitivity of these results toour choice of queries (6fti N and 6itc C), selected abovefor maximum symmetry, we repeated them with the op-posite half of each structure
  13. 16262551036386 1..32

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/cohesin%20folding%202021%20elife-67268-v1.pdf
    14 Jul 2021: decreasing it with Pds5. To test this, we measured the effect of scc4-4 in the presence or absence of.

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