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  2. Features critical for membrane binding revealed by DivIVA crystal…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/emboj201099a.pdf
    20 May 2010: membrane interaction. To test this, we purified the F17A. mutant and investigated membrane binding in vitro. ... could conveniently test suppressors for restored localization. The motivation for such screen was that suppressors of the.
  3. Membrane Localization is Critical for Activation of the PICK1 BAR…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/MadsenGetherTraffic2008.pdf
    18 Oct 2008: eYFP–PICK1 wt (middle row) or to-. gether with eYFP–PICK1 3KE (bottom. ... green (middle column). Right column. shows the merged pictures. White. bar 10 mm.
  4. Dissertation

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/AndyHuang.pdf
    6 Mar 2008: 74. Table 4.5: The results of the significance test (p-value < 0.05 is considered to be significant).
  5. Anne Bertolotti: Finding clues to combat neurodegenerative disease -…

    https://www2.mrc-lmb.cam.ac.uk/life-at-the-lmb/profiles/anne-bertolotti-finding-clues-to-combat-neurodegenerative-disease/
    Thumbnail for Anne Bertolotti: Finding clues to combat neurodegenerative disease - MRC Laboratory of Molecular Biology 21 Jul 2023: she says inadvertently became a test for how the LMB would perceive someone who wanted to be both a mother and a scientist. ... We don’t just want junior or middle-ranking women, we need women in leadership positions, and we still have a long way to go
  6. Using Coot for model building into EM maps:A tutorial ...

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/EM-Tutorial-Coot-PE.pdf
    8 Sep 2015: The test case for the tutorial is F420-reducing NiFe hydrogenase (Frh) {Allegretti:2014tz}. ... As a test case, we are going to use chain A from Frh.
  7. LMB 365 - Day 31 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-31%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... was officially handed over on 29 January 1962, and all 43 scientists had moved in by the middle of February.
  8. LMB 365 - Day 64 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-64%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... elegans (and eggs), with red and green fluorescence marking different tissues in the body, including neurons in the head
  9. LMB 365 - Day 53 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-53%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... 200),s.height=r):"link"===t.message&&(r=new URL(s.getAttribute("src")),n=new
  10. LMB 365 - Day 137 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-137%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... 200),s.height=r):"link"===t.message&&(r=new URL(s.getAttribute("src")),n=new
  11. LMB 365 - Day 146 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-146%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... 200),s.height=r):"link"===t.message&&(r=new URL(s.getAttribute("src")),n=new
  12. LMB 365 - Day 221 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-221%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... 200),s.height=r):"link"===t.message&&(r=new URL(s.getAttribute("src")),n=new
  13. LMB 365 - Day 167 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-167%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... over on 29 January 1962, and all 43 scientists had moved in by the middle of February.
  14. LMB 365 - Day 166 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-166%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... was officially handed over on 29 January 1962, and all 43 scientists had moved in by the middle of February.
  15. LMB 365 - Day 165 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-165%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... over on 29 January 1962, and all 43 scientists had moved in by the middle of February.
  16. LMB 365 - Day 163 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-163%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... over on 29 January 1962, and all 43 scientists had moved in by the middle of February.
  17. LMB 365 - Day 168 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-168%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... over on 29 January 1962, and all 43 scientists had moved in by the middle of February.
  18. The Coot User Manual

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/docs/coot.html
    1 Mar 2021: The Coot User Manual
  19. LMB 365 - Day 149 - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Flmb-365-day-149%2F&format=xml
    d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... The supporting metal helices in the middle of the stem have some parts where the nucleotide bases are left out,
  20. Phil. Trans. Roy. Soc. Lond. B. 261, 95-104 (1971) ...

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ptrs71.pdf
    26 Nov 2012: electron diffraction pattern: for the weakly scattering material we wish to examine, the high intensity of the central order beam would ensure that the potential at the middle of the thread ... The requirement now is that the potential at the middle of
  21. Bacterial actin MreB assembles in complex with cell shape protein RodZ

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embo2010RodZMreB.pdf
    17 Feb 2010: B) MreB polymerisation assays wereused to test the interface mutants of RodZ for MreB binding. ... structures from whichfilaments grow when mixed together in the presence of nucleotide and MgCl2 (middle panel).
  22. Insight into bacterial cell division: Architecture of the FtsZ ring - …

    https://www2.mrc-lmb.cam.ac.uk/wp-json/oembed/1.0/embed?url=https%3A%2F%2Fwww2.mrc-lmb.cam.ac.uk%2Finsight-bacterial-cell-division-architecture-ftsz-ring%2F&format=xml
    t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... n=new
  23. 61 Ultramicroscopy 25 (1988) 279-292 North-HoUand, Amsterdam 279…

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ultram88.pdf
    1 Apr 2016: In this paper, we examine the behaviour of the CTF for thin ice-embedded specimens within the framework of the hnear theory of image forma-tion, using as a test object ... correspond to 0.1 fim (left and middle) and 1/50 A " ' (right).
  24. 2623.tif

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb90.pdf
    23 Jan 2012: Sections at the horizontal line near the middle of the tube are shown in Fig. ... The ridges are quite prominent near the membrane but broad and smooth near the synaptic end; surface features are more complicated in the middle region.
  25. MinCD cell division proteins form alternating copolymeric cytomotive…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/ncomms6341.pdf
    6 Dec 2014: 1a,d). MinC and MinD copolymerize to form an alternatingcopolymer. To experimentally test filament formation, we usedfull-length, untagged E. ... A. Rapid pole-to-pole oscillation of a proteinrequired for directing division to the middle of Escherichia
  26. Coot Scripting Interface: cc-interface.hh Source File

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/docs/html/cc-interface_8hh_source.html
    24 Aug 2021: 615 // test the returned spec for unset_p().
  27. PNAS202120006_proof

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/pnas%20Lee%202022.pdf
    18 Dec 2022: To test thesehypotheses, we used the same N-terminal Brn1 release assay(Fig. ... 4. (A, Left and Middle) Condensin pentamer clamping circular plasmid DNA, as observed by cryo-EM with a VPP.
  28. Membrane transport between compartments in eukary-otic cells requires …

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Dynaminreview2004.pdf
    2 Feb 2004: Sequence homology does not allow us todefine the region between the middle domain and the. ... In humanMxA, a single point mutation (L612K) in the GEDblocks the interaction with the middle domain andinhibits oligomerization74.
  29. The structure of bactofilin filaments reveals their mode of membrane…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Deng2019Natmicro.pdf
    26 Aug 2019: Left, magnifications of the corners of a. Middle, magnifications of the corners of b with the TtBac sequence added beneath. ... coli. N. N. N. N. WT. Liposome. BactofilinBactofilin. Liposome. WT: middle of cell WT: top of cell.
  30. Thesis Chapter 0

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/Hardaker.pdf
    10 Jun 2008: To test the possible involvement of FLP-1-encoded peptides in the modulation of egg-.
  31. Qrb1300006 1..40

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/qrb13.pdf
    30 Jul 2013: twist around the tube axis). Preliminary tests using a segment size similar to that in Fig. ... 7b). As will be. described in Section 4.3, the gate of the channel is located near the middle of the membrane-.
  32. 30 Apr 2004 18:9 AR AR214-BB33-09.tex AR214-BB33-09.sgm…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/annrev2004.pdf
    1 May 2004: FtsZ is the organizingmolecule of bacterial cell division and forms a filamentous ring around the middle ofthe cell. ... At least theparC/ParR/M system is simple enoughto test this possibility in vitro.
  33. doi:10.1016/j.molcel.2008.05.027

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsK%20MolCell%202008%20Lowe.pdf
    16 Aug 2008: generally further away from the DNA with many water molecules. mediating contacts with the DNA (Figures 1E, middle, S1D, and. ... sequently activate recombination. In order to test whether the dimeric forms of the protein had.
  34. PII: S1097-2765(02)00515-4

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/SMC%20cohesin%20paper%202002.pdf
    19 Apr 2002: Upper two rows, open V conformation; middle two rows, Yconformation; lower row, coils spread conformation. ... To test this directly, we repeated the aboveexperiment using a yeast strain expressing Scc1myc18Cohesin Contains Only a Single Molecule.
  35. 1 Supplementary data to accompany paper: Mechanism of endophilin ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndophilinEMBO/EndoSupMethods.pdf
    8 Jun 2006: simple gaussian functions to dc/dt versus s curves to test for the number of components and. ... amphipathic helix are disordered, as well as a loop in the middle of the first helix which.
  36. Identifying proteins bound to native mitotic ESC chromosomes reveals…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Djeghloul%20Nat%20Camms%202020.pdf
    11 Aug 2020: P-values of statistically significant increases, measured by unpaired two tailed Student’s t-tests, are indicated. ... P-values of statisticallysignificant decreases, measured by unpaired two tailed Student’s t-tests, are indicated.
  37. 53091 361..366

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/McMahonE.pdf
    3 Dec 2004: endogenous AP2 (middle, AP6 antibody) and transferrin uptake (right). A strong. ... Liposome tubulation, sedimentation and electron microscopySynthetic liposomes contained 10% cholesterol, 40% phosphatidylethanolamine,40% phosphatidylcholine, and 10% of
  38. pone.0107211 1..10

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/leoa2014.pdf
    3 Sep 2014: tween the classical dynamin middle domain and GTPase effector. domain (GED) (Figure 1B). ... per other DFMs) a predicted membrane binding domain between. a middle domain and a GTPase effector domain (GED).
  39. A-nsmb855.indd

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nsmb%20FtsZ%202004.pdf
    19 Nov 2004: 2). The break in the protofilament is necessary for crystallization because the dimer formed by the 43-Å interaction (middle of Fig. ... To test for bound nucleotide, FtsZ was precipitated using 0.5 N cold HClO4, and after centrifugation nucleotide
  40. Molecular Biology of the CellVol. 8, 2003–2015, October 1997 ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/WiggeKohler_McMahon1997.pdf
    10 Oct 1997: To test this directly, Amph2 immunoprecipi-tates were Coomassie-stained, revealing that Amph1. ... This provided us with a useful tool to test theimportance of the heterodimer in vivo.
  41. Twinning and other pathologies Andrey Lebedev CCP4 OD-­‐structures…

    https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/%22https:/www2.mrc-lmb.cam.ac.uk/download/twinningaandaotherapathologiesa-andreyalebedeva-a/?wpdmdl=18788&
    11 Jun 2013: Twinning tests summary. Space group validaAon. 04/06/2013 58 Crystallography Course LMB. ... Twinning tests summary. Space group validaAon. 04/06/2013 61 Crystallography Course LMB.
  42. Wild card macros

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/sciencegayathri2012/Gayathri.SM.pdf
    23 Oct 2012: parenthesis. † Rmerge = ΣΣ | Ihl - 〈Ih〉 | / ΣΣ 〈Ih〉 h l h l ‡ 5 % of the reflections were included in the test set. ... The middle segments (initial seed) of the filaments become photobleached as the movie progresses, while the intensity
  43. doi:10.1016/j.cell.2006.01.047

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/16615893.pdf
    18 Apr 2006: through an adaptor protein. To test whether these two. complexes can directly interact in vitro, we used analyt-. ... Avanti or Echelon). Control vesicles contained an additional 10% PC. instead of the test lipid.
  44. 2168 | K. von der Malsburg et al. Molecular ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/93_vonderMalsburg-_K_MBC_2015.pdf
    11 Aug 2015: To test this directly, we used nondenaturing affinity purification to assess a possible 60S-listerin-translocon ternary complex (Figure 1E). ... NCtRNA. NC. 87654321. 87654321. 654321. (middle) E1 and E2 were analyzed by SDS–PAGE and autoradiography.
  45. doi:10.1016/j.str.2007.05.002

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/FCH02F-BARHenne07.pdf
    2 Jun 2007: protein saturation on liposomes. To test more directly whether the dimer seen in the crys-. ... tutive helix. To test for a role for helix 1 in membrane bind-.
  46. Functional Depletion of Mahogunin by Cytosolically Exposed Prion…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/59_Chakrabarti_O_Cell_2009.pdf
    13 Jun 2009: an in vivo interaction assay based on coassociation of an. FP-tagged test protein with FP-tagged cytosolic PrP aggregates. ... involving excessive PrP exposure to the cytosol. To test this. idea, we analyzed the status of Mgrn and lysosomes in trans-.
  47. The GTPase-Activating Protein GRAF1 Regulates the CLIC/GEEC Endocytic …

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Lundmark_McMahon_GRAF2008.pdf
    28 Nov 2008: The error bars show 95% confidence intervals (calculated by t tests) for each condition. ... top), internalized anti-GFP (middle), and a merged, triple-labeled image (bottom). Arrows indicate points of colocalization.
  48. doi:10.1016/j.molcel.2004.08.030

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Haering%20Mol%20Cell%202004.pdf
    20 Sep 2004: However, the disorder makes it difficult todesign specific mutants to test this hypothesis.its C terminus (Figure 1D). ... To test whether the S525N mutation Is There Any Turnover of Cohesin Subunits.
  49. A positively charged channel within the Smc1/Smc3 hinge required for…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embosmcjingekurze2010.pdf
    1 Dec 2010: form a pseudo twofold symmetric torus with a small channel. in the middle. ... generate sister chromatid cohesion. To test the effect of charge neutralization on cohesin complex.
  50. JFCregg_OGS_finaledit

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/Cregg_thesis.pdf
    14 Dec 2010: Toledo, Ohio, in the middle of winter and, finally, to San Francisco. ... After the. UK was a stop in Toledo, Ohio, in the middle of winter, not my top choice of places to.
  51. elife.elifesciences.org MacKinnon et al. eLife 2014;3:e01483. DOI:…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/83_MacKinnon_AL_eLife_2014.pdf
    9 Sep 2015: Comparison of sensitive and resistant TMDs at multiple points along the integration pathway (i.e., at multiple nascent chain lengths) should allow us to: (1) test whether they pass through a

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