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18 Dec 2008: 1C, middle panel). Immunostain-ing with antibodies to Saci1373 (ESCRT-III). revealed a distinct subcellular localization ofthe protein. ... To further test the invivo role of the Sulfolobus ESCRTcomponents, wegenerated an episomal vector to direct

13 Dec 2008: inclusion reaching its middle (Fig. 4 b) will not result in themembrane bending since the upper monolayer can expand. ... 5). To test the sensitivity of the model. predictions to this assumption, we repeated the calculations.

22 Jul 2007: F-actin stress fibres (Figure 2E, middle panel compared with. uninfected cells or cells infected with retrovirus expressing. ... In addition,. using mouse models as a preclinical test, as we have done in.

3 Dec 2005: previously used for the egg detection test and 20 new normal wild type videos. ... in 5 sec CANGCRMAX Max angle change rate in middle sect.TLMV10AVG Avg.

16 Jul 2023: S1E). As a further test of thisfinding, we expressed the Vps4E209Q plasmid in a cell line carryinga thymidine kinase homolog (hereafter called STK). ... 3A).To test the functional significance of this patterned assembly ofESCRT-III polymers, we used

14 Jun 2006: Middle) Plot of the similarity between PI3-Ks according to their IC50 values against small molecule. ... cell culture are recapitulated in vivo by performing insulin. tolerance tests in mice.

cam.ac.uk/venki-ramakrishnan-world-economic-forum-davos/">Venki Ramakrishnan at World Economic Forum in Davos</a></blockquote><iframe sandbox="allow-scripts" security="restricted" ... The LMB's Venki Ramakrishnan took part in a panel discussion on the

24 Jan 2005: Theinner M2 helices also change their configuration inresponse to ACh, widening the lumen of the pore atthe middle of the membrane. ... The model-derivedphases provided a more sensitive test of the signalretained in the images at high resolution than

27 Mar 2012: and Errington, 2009). FtsZ finds the middle of the cell. through two independent mechanisms in E. ... colicells and imaged by electron cryotomography. EcFtsA without the amphipathic helix (top left) forms long, straight bundles in the middle of thecell

12 Oct 2012: Imageswere obtained with a cooled EMCCD camera (9100-02; Hamamatsu). Statistics. Significance was tested using the 2-tailed Student t test. ... Pseudocolored fluorescence image ofCOS-7 cells expressing EGFP alone (left), WT EGFP-PSTPIP2 (middle) or

4 Oct 2007: tion. Mutant parR genes were tested for functionality in their. natural genetic context on test plasmids derived from low-. ... As shown in Figure 4A, test plasmids. containing the ParR171 K6E, R7S and K8E mutations severely.

30 Jun 2004: parS. sequence5. ¢. -TGTTCCACGTGAAACA-3. ¢. (Fig. 1A and B). To test forthe ability of wild-type and truncated proteins to carry outDNA binding and spreading, we used an agarose gel ... Groups of 12 scans were taken shortly after theboundary cleared

21 Nov 2014: Scc1 contains many more residues in the middle domain. Separase cleavage sites, Pds5(7) and Scc3 binding sites are highlighted (28). ... S7E). Sister DNAs are entrapped bycohesin trimers. To test whether cross-linking all three interfacesof the cohesin

15 Mar 2018: thetic complex. To test this hypothesis, we examined MreB motions as we changed the shape of. ... increase with cell width (Ouzounov et al., 2016). To test if the angle of MreB movement changes.

18 Feb 2016: Thus, the idea emerged that mRNAs are ‘tested’ for their integrity by translation, and failure of this test initiates their degradation to prevent production of faulty proteins1,31. ... An advantage of encoding an arrest-inducing sequence in the

11 Nov 2011: The aggregated protein indicated at left was coexpressed with the test protein indicated along the top. ... At each time point, the % of aggregate-containing cells that showed excess intracellular accumulation of the test protein is indicated.

9 Nov 2001: Testing them will involve addressing ques-tions such as the interactions that localize T-tubules tothe middle of the M–Z interval, the binding partners ofthe SH3 domain of Drosophila amphiphysin ... For complementation tests, amph26 homozygoteswere

21 Feb 2011: To test these activities, wecoexpressed full-length Rvs161 and Rvs167 using an E. ... To-gether, our biochemical tests show that Rvs161-Rvs167 bindsto membranes and induces three-dimensional curvature.

19 Dec 2016: helical 2D class average (top), re-projections of F-actin double-stranded filament structure (middle) (von der Ecken et al., 2015) and crenactin single. ... crenactin filaments. To test this, we performed. the same experiment using a peptide spanning.

17 Jun 2005: ease of vesicles that were not primed at the time theeptide was introduced.To test whether a population of vesicles were primed. ... irst stimulus (upper), the percentage of excess membrane re-rieved during the fast phase (middle), and the rate constant

15 Apr 2004: The left and middle panels used directly conjugated anti-CNX antibodies against the COOH and NH2 terminus, respectively. ... multiple days.aTreatment is significant (P 0.01) compared with untreated cells using t test.

8 Jun 2006: implying the formation of additional helical structures. To test. how the predicted N-terminal amphipathic helix folds,. ... seeResults). Student’s t-test Po0.001, Po0.2. Endophilin and vesicle buddingJL Gallop et al. &

22 Mar 2005: EpsinR was localized to endosomal cells that were recognized via thick tubular elements inmembranes, and involved in the retrograde transport of their cytoplasm (Figure 1G, middle). ... To test whether AP1 is required for the retro- dress its function in

20 Jun 2009: Horizontal black barsindicate the mean values for each data set. Each mutant dataset was compared to wtPrP by the Student’s t-test and found to be statisticallysignificant in all cases

17 Mar 2003: of polymerized clathrin triskelia making a lattice of hexa-gons and pentagons, a middle layer where clathrin and To whom correspondence should be addressed (e-mail: hmm@. ... In order to test the validity of this hypoth-by six residues to complete the b

10 Jan 2013: A side view is shown in the middle and a view of theconcave surface on the bottom. ... Test set size (%) 5.4. Rwork (%) 19.6(22.9). Rfree (%) 25.2(23.8).

21 Feb 2011: middle of the stalk and contributes similarly to theenergies of both fusion and fission stalks [3,73].[(Box_1)TD$FIG]. ... Science 321, 1354–1357. 62 Sever, S. et al. (2000) Garrotes, springs, ratchets, and whips: puttingdynamin models to the test.

3 Sep 2008: Second, a molecule from the A layer of one. strand uses its a1/a2 loop to contact the middle of helix a2 of. ... some uncertainty of the molecular interpretation of the filaments,. so we carried out site-specific mutagenesis to test the contacts.

7 Jul 1999: The middle sphere lies between the side-chains of Asp2 of one molecule in the asymmetricunit and a symmetry-related copy of the other mol-ecule in the asymmetric unit. ... The residuals betweenthe tted absorbance and the experimental values wereplotted,

4 Aug 2017: middle) or physical interactions (bottom). ... Fig. 2. Reconstitution ofUBE2O-mediated clientubiquitination with purifiedfactors. (A) Experimentalstrategy to test UBE2O inter-action and ubiquitination in adefined system.

28 Sep 2007: later). Following the middle domain, a40-residue linker connects the helical domain with the C-terminalEH-domain (residues 443–543). ... S, supernatant; P, pelleted fraction.d, Electron micrographs of negatively stainedPtdSer liposomes in the absence

7 Apr 2010: As a result, a membranefragment with an amphipathic helix in the middle has an effectivefunnel-like shape, which can fit to a sphere with a certain radiusand the corresponding total curvature.

20 Nov 2010: The fusion pathway consists of. membrane dimpling leading to the generation of curvature stresses and the establishment of point-like intermembrane contacts (left panel), fusion stalk formation (middle. ... Science 321, 1354–1357. 62 Sever, S. et al.

27 Jan 2018: MEMBRANE TARGETING. The ER membrane protein complex isa transmembrane domain insertaseAlina Guna,1 Norbert Volkmar,2 John C. Christianson,2 Ramanujan S. Hegde1†. Insertion of proteins into membranes is an essential cellular process. The

23 Oct 2012: parenthesis. † Rmerge = ΣΣ | Ihl - 〈Ih〉 | / ΣΣ 〈Ih〉 h l h l ‡ 5 % of the reflections were included in the test set. ... The middle segments (initial seed) of the filaments become photobleached as the movie progresses, while the intensity

11 Aug 2015: To test this directly, we used nondenaturing affinity purification to assess a possible 60S-listerin-translocon ternary complex (Figure 1E). ... NCtRNA. NC. 87654321. 87654321. 654321. (middle) E1 and E2 were analyzed by SDS–PAGE and autoradiography.

13 Jun 2009: an in vivo interaction assay based on coassociation of an. FP-tagged test protein with FP-tagged cytosolic PrP aggregates. ... involving excessive PrP exposure to the cytosol. To test this. idea, we analyzed the status of Mgrn and lysosomes in trans-.

28 Nov 2008: The error bars show 95% confidence intervals (calculated by t tests) for each condition. ... top), internalized anti-GFP (middle), and a merged, triple-labeled image (bottom). Arrows indicate points of colocalization.

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30 Apr 2024: 1 A, Middle) and a symmetrical rim of MX helices lying flat against the inner (cytoplasmic) bilayer surface (Fig. ... B) In-plane slabs through densities and superimposed model cutting through the top, middle and bottom portions of the TM helices.

8 Aug 2014: and Zr Scc3. (A) Zr Scc3 structure, same as Fig. 1A, middle, colored blue to red from N- to C-. ... flow through, b: bound). (B) To test if Sc Scc3 forms homodimers, full length.

7 Jun 2022: squares). Cancers from the transgenic APC mouse (left) are larger than those found in the APC mice that lack IL-25 (middle) or ILC2s (right). ... Further tests on human CRC tissue, provided by Simon Buczacki at Addenbrooke’s Hospital, Cambridge (now at

20 May 2010: membrane interaction. To test this, we purified the F17A. mutant and investigated membrane binding in vitro. ... could conveniently test suppressors for restored localization. The motivation for such screen was that suppressors of the.

28 Sep 2020: see the video– Ctrl <middle-mouse> scroll. 4 / 50. “3D sticks in space” is the way in which Coot 0.0-0.9.1 has represented models and maps. ... 28 / 50. Atoms Clashing. Test-bed/Hello World. Ribosomes at 60FPS. Ribosomes at 60FPS.

6 Mar 2008: 74. Table 4.5: The results of the significance test (p-value < 0.05 is considered to be significant).

18 Oct 2008: eYFP–PICK1 wt (middle row) or to-. gether with eYFP–PICK1 3KE (bottom. ... green (middle column). Right column. shows the merged pictures. White. bar 10 mm.

21 Jul 2023: she says inadvertently became a test for how the LMB would perceive someone who wanted to be both a mother and a scientist. ... We don’t just want junior or middle-ranking women, we need women in leadership positions, and we still have a long way to go

1 Mar 2021: The Coot User Manual

8 Sep 2015: The test case for the tutorial is F420-reducing NiFe hydrogenase (Frh) {Allegretti:2014tz}. ... As a test case, we are going to use chain A from Frh.

d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[a-zA-Z0-9]/.test(t.secret)){for(var ... was officially handed over on 29 January 1962, and all 43 scientists had moved in by the middle of February.