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19 Apr 2002: Upper two rows, open V conformation; middle two rows, Yconformation; lower row, coils spread conformation. ... To test this directly, we repeated the aboveexperiment using a yeast strain expressing Scc1myc18Cohesin Contains Only a Single Molecule.

26 Aug 2019: Left, magnifications of the corners of a. Middle, magnifications of the corners of b with the TtBac sequence added beneath. ... coli. N. N. N. N. WT. Liposome. BactofilinBactofilin. Liposome. WT: middle of cell WT: top of cell.

10 Jun 2008: To test the possible involvement of FLP-1-encoded peptides in the modulation of egg-.

30 Jul 2013: twist around the tube axis). Preliminary tests using a segment size similar to that in Fig. ... 7b). As will be. described in Section 4.3, the gate of the channel is located near the middle of the membrane-.

11 Aug 2020: P-values of statistically significant increases, measured by unpaired two tailed Student’s t-tests, are indicated. ... P-values of statisticallysignificant decreases, measured by unpaired two tailed Student’s t-tests, are indicated.

1 May 2004: FtsZ is the organizingmolecule of bacterial cell division and forms a filamentous ring around the middle ofthe cell. ... At least theparC/ParR/M system is simple enoughto test this possibility in vitro.

18 Apr 2006: through an adaptor protein. To test whether these two. complexes can directly interact in vitro, we used analyt-. ... Avanti or Echelon). Control vesicles contained an additional 10% PC. instead of the test lipid.

10 Oct 1997: To test this directly, Amph2 immunoprecipi-tates were Coomassie-stained, revealing that Amph1. ... This provided us with a useful tool to test theimportance of the heterodimer in vivo.

3 Dec 2004: endogenous AP2 (middle, AP6 antibody) and transferrin uptake (right). A strong. ... Liposome tubulation, sedimentation and electron microscopySynthetic liposomes contained 10% cholesterol, 40% phosphatidylethanolamine,40% phosphatidylcholine, and 10% of

19 Nov 2004: 2). The break in the protofilament is necessary for crystallization because the dimer formed by the 43-Å interaction (middle of Fig. ... To test for bound nucleotide, FtsZ was precipitated using 0.5 N cold HClO4, and after centrifugation nucleotide

3 Sep 2014: tween the classical dynamin middle domain and GTPase effector. domain (GED) (Figure 1B). ... per other DFMs) a predicted membrane binding domain between. a middle domain and a GTPase effector domain (GED).

1 Dec 2010: form a pseudo twofold symmetric torus with a small channel. in the middle. ... generate sister chromatid cohesion. To test the effect of charge neutralization on cohesin complex.

20 Sep 2004: However, the disorder makes it difficult todesign specific mutants to test this hypothesis.its C terminus (Figure 1D). ... To test whether the S525N mutation Is There Any Turnover of Cohesin Subunits.

2 Jun 2007: protein saturation on liposomes. To test more directly whether the dimer seen in the crys-. ... tutive helix. To test for a role for helix 1 in membrane bind-.

19 Mar 2013: 3C). To further test for auto-inhibition, we per-formed a series of co-sedimentation assays with SUVs (Fig. ... 42). Although our assay does notallow us to test for possible scission mechanisms, we can ask thefollowing question.

3 Dec 2004: endogenous AP2 (middle, AP6 antibody) and transferrin uptake (right). A strong. ... Liposome tubulation, sedimentation and electron microscopySynthetic liposomes contained 10% cholesterol, 40% phosphatidylethanolamine,40% phosphatidylcholine, and 10% of

13 Dec 2008: inclusion reaching its middle (Fig. 4 b) will not result in themembrane bending since the upper monolayer can expand. ... 5). To test the sensitivity of the model. predictions to this assumption, we repeated the calculations.

14 Dec 2010: Toledo, Ohio, in the middle of winter and, finally, to San Francisco. ... After the. UK was a stop in Toledo, Ohio, in the middle of winter, not my top choice of places to.

22 Jul 2007: F-actin stress fibres (Figure 2E, middle panel compared with. uninfected cells or cells infected with retrovirus expressing. ... In addition,. using mouse models as a preclinical test, as we have done in.

12 Oct 2012: Imageswere obtained with a cooled EMCCD camera (9100-02; Hamamatsu). Statistics. Significance was tested using the 2-tailed Student t test. ... Pseudocolored fluorescence image ofCOS-7 cells expressing EGFP alone (left), WT EGFP-PSTPIP2 (middle) or

16 Jul 2023: S1E). As a further test of thisfinding, we expressed the Vps4E209Q plasmid in a cell line carryinga thymidine kinase homolog (hereafter called STK). ... 3A).To test the functional significance of this patterned assembly ofESCRT-III polymers, we used

14 Jun 2006: Middle) Plot of the similarity between PI3-Ks according to their IC50 values against small molecule. ... cell culture are recapitulated in vivo by performing insulin. tolerance tests in mice.

18 Feb 2016: Thus, the idea emerged that mRNAs are ‘tested’ for their integrity by translation, and failure of this test initiates their degradation to prevent production of faulty proteins1,31. ... An advantage of encoding an arrest-inducing sequence in the

21 Nov 2014: Scc1 contains many more residues in the middle domain. Separase cleavage sites, Pds5(7) and Scc3 binding sites are highlighted (28). ... S7E). Sister DNAs are entrapped bycohesin trimers. To test whether cross-linking all three interfacesof the cohesin

4 Oct 2007: tion. Mutant parR genes were tested for functionality in their. natural genetic context on test plasmids derived from low-. ... As shown in Figure 4A, test plasmids. containing the ParR171 K6E, R7S and K8E mutations severely.

27 Mar 2012: and Errington, 2009). FtsZ finds the middle of the cell. through two independent mechanisms in E. ... colicells and imaged by electron cryotomography. EcFtsA without the amphipathic helix (top left) forms long, straight bundles in the middle of thecell

11 Nov 2011: The aggregated protein indicated at left was coexpressed with the test protein indicated along the top. ... At each time point, the % of aggregate-containing cells that showed excess intracellular accumulation of the test protein is indicated.

8 Jun 2006: implying the formation of additional helical structures. To test. how the predicted N-terminal amphipathic helix folds,. ... seeResults). Student’s t-test Po0.001, Po0.2. Endophilin and vesicle buddingJL Gallop et al. &

15 Apr 2004: The left and middle panels used directly conjugated anti-CNX antibodies against the COOH and NH2 terminus, respectively. ... multiple days.aTreatment is significant (P 0.01) compared with untreated cells using t test.

20 Jun 2009: Horizontal black barsindicate the mean values for each data set. Each mutant dataset was compared to wtPrP by the Student’s t-test and found to be statisticallysignificant in all cases

10 Jan 2013: A side view is shown in the middle and a view of theconcave surface on the bottom. ... Test set size (%) 5.4. Rwork (%) 19.6(22.9). Rfree (%) 25.2(23.8).

21 Feb 2011: To test these activities, wecoexpressed full-length Rvs161 and Rvs167 using an E. ... To-gether, our biochemical tests show that Rvs161-Rvs167 bindsto membranes and induces three-dimensional curvature.

17 Jun 2005: ease of vesicles that were not primed at the time theeptide was introduced.To test whether a population of vesicles were primed. ... irst stimulus (upper), the percentage of excess membrane re-rieved during the fast phase (middle), and the rate constant

24 Jan 2005: Theinner M2 helices also change their configuration inresponse to ACh, widening the lumen of the pore atthe middle of the membrane. ... The model-derivedphases provided a more sensitive test of the signalretained in the images at high resolution than

30 Jun 2004: parS. sequence5. ¢. -TGTTCCACGTGAAACA-3. ¢. (Fig. 1A and B). To test forthe ability of wild-type and truncated proteins to carry outDNA binding and spreading, we used an agarose gel ... Groups of 12 scans were taken shortly after theboundary cleared

28 Sep 2007: later). Following the middle domain, a40-residue linker connects the helical domain with the C-terminalEH-domain (residues 443–543). ... S, supernatant; P, pelleted fraction.d, Electron micrographs of negatively stainedPtdSer liposomes in the absence

22 Mar 2005: EpsinR was localized to endosomal cells that were recognized via thick tubular elements inmembranes, and involved in the retrograde transport of their cytoplasm (Figure 1G, middle). ... To test whether AP1 is required for the retro- dress its function in

20 Nov 2010: The fusion pathway consists of. membrane dimpling leading to the generation of curvature stresses and the establishment of point-like intermembrane contacts (left panel), fusion stalk formation (middle. ... Science 321, 1354–1357. 62 Sever, S. et al.

9 Nov 2001: Testing them will involve addressing ques-tions such as the interactions that localize T-tubules tothe middle of the M–Z interval, the binding partners ofthe SH3 domain of Drosophila amphiphysin ... For complementation tests, amph26 homozygoteswere

15 Mar 2018: thetic complex. To test this hypothesis, we examined MreB motions as we changed the shape of. ... increase with cell width (Ouzounov et al., 2016). To test if the angle of MreB movement changes.

21 Feb 2011: middle of the stalk and contributes similarly to theenergies of both fusion and fission stalks [3,73].[(Box_1)TD$FIG]. ... Science 321, 1354–1357. 62 Sever, S. et al. (2000) Garrotes, springs, ratchets, and whips: puttingdynamin models to the test.

17 Mar 2003: of polymerized clathrin triskelia making a lattice of hexa-gons and pentagons, a middle layer where clathrin and To whom correspondence should be addressed (e-mail: hmm@. ... In order to test the validity of this hypoth-by six residues to complete the b

19 Dec 2016: helical 2D class average (top), re-projections of F-actin double-stranded filament structure (middle) (von der Ecken et al., 2015) and crenactin single. ... crenactin filaments. To test this, we performed. the same experiment using a peptide spanning.

3 Sep 2008: Second, a molecule from the A layer of one. strand uses its a1/a2 loop to contact the middle of helix a2 of. ... some uncertainty of the molecular interpretation of the filaments,. so we carried out site-specific mutagenesis to test the contacts.

11 Aug 2015: To test this directly, we used nondenaturing affinity purification to assess a possible 60S-listerin-translocon ternary complex (Figure 1E). ... NCtRNA. NC. 87654321. 87654321. 654321. (middle) E1 and E2 were analyzed by SDS–PAGE and autoradiography.

7 Jul 1999: The middle sphere lies between the side-chains of Asp2 of one molecule in the asymmetricunit and a symmetry-related copy of the other mol-ecule in the asymmetric unit. ... The residuals betweenthe tted absorbance and the experimental values wereplotted,

13 Jun 2009: an in vivo interaction assay based on coassociation of an. FP-tagged test protein with FP-tagged cytosolic PrP aggregates. ... involving excessive PrP exposure to the cytosol. To test this. idea, we analyzed the status of Mgrn and lysosomes in trans-.

28 Nov 2008: The error bars show 95% confidence intervals (calculated by t tests) for each condition. ... top), internalized anti-GFP (middle), and a merged, triple-labeled image (bottom). Arrows indicate points of colocalization.

23 Oct 2012: parenthesis. † Rmerge = ΣΣ | Ihl - 〈Ih〉 | / ΣΣ 〈Ih〉 h l h l ‡ 5 % of the reflections were included in the test set. ... The middle segments (initial seed) of the filaments become photobleached as the movie progresses, while the intensity

9 Sep 2015: Comparison of sensitive and resistant TMDs at multiple points along the integration pathway (i.e., at multiple nascent chain lengths) should allow us to: (1) test whether they pass through a