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Expression of selenomethionine substituted proteins innon-methionine…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/methods/SeMet%20recipe.doc.pdf2 Feb 2004: J Mol Biol. 1993 Jan 5;229(1):105-24. The protein is expressed in any E. -
News - Cambs 24
https://www2.mrc-lmb.cam.ac.uk/archive/articles/Road_named_after_legendary_scientist.pdf1 Feb 2013: Copyright 2013 Archant Regional Ltd. All rights reserved. News - Cambs 24 http://www.cambs24.co.uk/news/cambridge_road_to_be_na. -
Impress
https://www2.mrc-lmb.cam.ac.uk/coot/ephemera/beyond-coloured-lines.pdf28 Sep 2020: Aids). 23 / 50. Screenshots. 24 / 50. Generic Objects. 25 / 50. ... Slide 24. Slide 25. Slide 26. Slide 27. Slide 28. Slide 29. -
Structure of a cholinergic cell membraneNigel Unwina,1 Edited by ...
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnasS22.pdf4 Nov 2022: The broken yellow lines identify the well-documented ribbon of δ subunit-linked receptor dimers(14, 15, 18, 24), one of which is labeled in each leaflet to indicate the subunit ... Biophys. J. 92, 3960–3967 (2007). 24. H. W. Chang, E. Bock, Molecular -
Phosphoinositide-Specific Phospholipase Cδ1 Activity toward Micellar…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9287165.pdf20 Apr 2002: diC7PC (8 mM) no significant activation observed 0.85( 0.06 24.7( 2.9(1.7( 0.2). ... 132) PI-PLCδ1 and at 24-45 C for full-length PI-PLCδ1 (Figure 7A,B). -
The Jour nal o f Cel l Bio logy ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/26_Snapp_EL_JCB_2003.pdf16 Oct 2003: The. Jour. nal o. f Cel. l Bio. logy. JCB. Article. The Journal of Cell Biology, Volume 163, Number 2, October 27, 2003 257–269http://www.jcb.org/cgi/doi/10.1083/jcb.200306020 257. Formation of stacked ER cisternae by low affinity protein -
doi:10.1016/j.ceb.2003.11.005
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/cocb%202003.pdf27 Nov 2003: Myosin is then recruited [24] andactin bundles are assembled, organized by formins [14].Acto-myosin sliding-filament contraction constricts thecell diameter until it is filled by the central-spindlebundle of microtubules ... nomenclature. Mol Biol Cell -
Bacterial chromosome segregation: structure andDNA binding of the Soj …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/soj_embo2005.pdf30 Jan 2005: The EMBO Journal VOL 24 | NO 2 | 2005 & 2005 European Molecular Biology Organization. ... The EMBO Journal VOL 24 | NO 2 | 2005 & 2005 European Molecular Biology Organization272. -
Influence of lipid bilayer on the structure of the muscle-type…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnasS24.pdf30 Apr 2024: in cell- derived membrane vesicles (24), or by in situ cryoelectron tomography (25). ... Electron Microscopy Data Bank https://www.ebi.ac.uk/emdb/EMD-18845. Deposited 6 November 2023. 24. N.Unwin, Influence of lipid bilayer on structure of acetylcholine -
The Journal of Cell Biology © The Rockefeller University ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsinR/sedequil.pdf16 Jan 2003: Afteran initial scan, the centrifuge was over-speeded at 24,000 rev/min for 6 h, to reduce the time taken to reach equilibrium (Van Holde and Baldwin, 1958),and then ... the speed was reduced and another scan was taken, followed by further scans at -
The Journal of Cell Biology © The Rockefeller University ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/sedequil.pdf16 Jan 2003: Afteran initial scan, the centrifuge was over-speeded at 24,000 rev/min for 6 h, to reduce the time taken to reach equilibrium (Van Holde and Baldwin, 1958),and then ... the speed was reduced and another scan was taken, followed by further scans at -
Sup Fig 1
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/YopJSup.pdf11 Nov 2008: 24 h after transfection cells were serum-starved for 16 h and then stimulated with 20 ng/ml TNF-α. ... 24. 26. 28. 30. 32. 34. 36. 38. 40. 42. inte. -
�������� ��� � ���� �� �� ���� ��� ������ ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsA%20EMBO%20.pdf4 Oct 2000: E#N# 04412 (B<-! A # # " 3 16G7H#. # >$$I# ># 3 J#3# 04492 ' ( ' ) % % # ) &. 2'99G997#. $# B # O (# 0442 ( (- $ $ 6H $ # # / 0/ 24 64HG647#. (#-# -
PII: S0014-5793(01)02216-5
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MinD%20FEBS.pdf5 Mar 2001: 1990) Annu.Rev. Genet. 24, 249274. [3] Lutkenhaus, J. and Addinall, S.G. ... Appl. Crystallogr. 24, 946950.[34] Merritt, E.A. and Bacon, D.J. (1997) in : Macromolecular Crys-. -
bic079a
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/DonaldsonJ.pdf11 Aug 2008: In cells, the Arf GTP-bind-ing and hydrolysis cycle is regulated by GEFs (guanine-nucleo-tide-exchange factors) and GAPs (GTPase-activating proteins).There are 14 GEFs and 24 GAPs ... EMBO J. 24,2244–2253. 10 Peter, B. J., Kent, H. M., Mills, I. -
doi:10.1016/j.pbiomolbio.2004.07.009
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/stock2005robot.pdf19 Jan 2005: Quik phosphate grid screen 24 Hampton Research. NaCl grid screen 24 Hampton Research. ... The platform also accommodates most commercially availablecrystallisation plates (24-well Linbro (Hampton Research, #HR3-110), 24-well Cryschem(Hampton Research, -
Microscopy, 2021, 1–6doi:https://doi.org/10.1093/jmicro/dfab023…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/mic21.pdf26 Jul 2021: acetylcholine receptor clusters in C2C12 myotubes. J. Lipid. Res. 47:2121–2123. 24. -
Journal of Visualized Experiments www.jove.com Copyright © 2018…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/JOVE2018.pdf19 Apr 2018: JBS6 JenaBioScience CS-106L 24 Incomplete factorial based on AmS (pH 4.6-8.5). ... JBS7 JenaBioScience CS-107L 24 Incomplete factorial based on MPD (pH 4.6-8.5). -
PII: 0304-3991(84)90197-9
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ultram84.pdf26 Oct 2008: The capacity of the Dewar is sufficient to give a running time of about 24 h under these conditions. -
Nature © Macmillan Publishers Ltd 1998 8 30. Fan, ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsZ.Nature.pdf9 Jan 1998: Biochem. 24, 255–302 (1995). 22. Leslie, A. G. W. Recent changes to the MOSFLM package for processing film and image plate data (SERCLaboratory, Daresbury, Warrington, UK, 1991). ... 23. The CCP4 suite: Programs for protein crystallography. Acta -
KWRM_A_992668_O
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-3.pdf20 Oct 2015: Submitted: 11/06/2014. Revised: 11/21/2014. Accepted: 11/24/2014. http://dx.doi.org/10.4161/21624054.2014.992668This is an Open Access article distributed under theterms of the ... Molecular anatomy of a trafficking organelle.Cell 2006; 127:831-46; -
1 CURRICULUM VITAE Venki Ramakrishnan Nationality U.S. (since 1985)…
https://www2.mrc-lmb.cam.ac.uk/groups/ribo/download/ramakrishnan_cv_4.pdf4 Jan 2024: Nat Struct Mol Biol 24, 866-869. N. R. James, A. Brown, Y. ... 123, 24-34. Gerchman, S.E. and Ramakrishnan, V. (1987). Chromatin higher-order structure studied by neutron scattering and scanning transmission electron microscopy. -
MD1-118 Hippocrates additive screen
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/Addit-03.pdf13 Oct 2020: D3 21 1.5 % Caffeic acid †D4 22 1.5 % D-()-Quinic acidD5 23 1.5 % Gallic acid monohydrate†D6 24 1.5 % N-vanillylnonanamide (capsaicin synthetic)†E1 25 1.5 % -
Molecular basis for membraneremodelling and organization Deadline for …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/F-BAR_proteins/Le%20Poster_DEUXNew.pdf29 Sep 2011: 24-28 September 2011. Main Topics:Membrane curvature invesicle fusion. Membrane curvature in endocytosis and vesicle budding. -
struct1345mmc1
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/FCH02F-BAR_SUP07.pdf2 Jun 2007: After the overspeeding, interference scans were taken at 24 h intervals, and, when. -
A Large Particle Associated with the Perimeter of the ...
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb82.pdf5 Jul 2010: Best preserved porecomplexes show evidence of 16- and 24-fold harmonics inaddition to the basic eightfold harmonic (Fig. ... J. CellBiol. 52:338-354. 24. Paine, P. L. , L, C. Moore, and S. -
LMB Screen Scoring Sheet
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/LMB_21.pdf23 Sep 2016: B9 18% v/v ethanol, 0.1M bis tris propane pH 7.1. B10 24% v/v ethanol, 0.04M magnesium chloride hexahydrate, 0.1M HEPES pH 7.8. ... D7 15% w/v PEG 3350, 0.1M MES pH 6.2. D8 24% w/v PEG 3350, 0.15M sodium chloride, 0.05M HEPES pH 6.8, 2% v/v -
Structure of bacterial tubulin BtubA�B:Evidence for horizontal gene…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/btubab%202005.pdf21 Jun 2005: BtubAB form the same het-erodimer as tubulin (24, 25). The protofilament axis isvertical. ... 1993) Micron 24, 465– 471. 10. Petroni, G., Spring, S., Schleifer, K.-H., Verni, F. & -
53091 361..366
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/McMahonE.pdf3 Dec 2004: 24). Thus our experiments point to a pioneering role forepsin in vesicle budding as it provides both a driving force and a linkbetween membrane invagination, clathrin polymerization and AP2complex recruitment. ... beta 2-adaptin appendage domain. EMBO J. -
Crystal structure of the SOS cell division inhibitorSulA and ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/SulA%20PNAS%202003.pdf13 Jun 2003: To whom correspondence should be addressed. E-mail: jyl@mrc-lmb.cam.ac.uk. www.pnas.orgcgidoi10.1073pnas.1330742100 PNAS June 24, 2003 vol. ... Lowe, J. (1999) Struct.Folding Des. 7, 1181–1187. 24. Van Duyne, G. -
S413_6a 39..44
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MreB%20Nature.pdf30 Aug 2001: 51.1 Å. IA. IBIIB. IIA. F-actin MreB. 55 Å. 1. 24. ... Interestingly, the rened model ofF-actin23,24 is less similar to our crystal structure than the initialunrened model, in which the crystal structure of G-actin incomplex with DNase I was -
Cryo-EM reconstruction of AlfA from Bacillus subtilis reveals the…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/AlfA2018.pdf20 Mar 2018: The subunits are staggered in a left-handed, double-heli-cal, parallel (polar) filament, with a rise of 24.9 Å and a 156.5 twist. ... Science 338:1334–1337. 24. Schumacher MA, et al. (2007) Segrosome structure revealed by a complex of ParRwith -
se289900215p
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/strucrev.pdf6 Jul 1999: Adaptors EM, rotary shadow and deep-etch; APcomplexes (13). ;25 (2D). Crystallography; AP2 m2 residues 158 to435 (24). ... 2.7 Phe174, Arg423 (24) YxxØ endocytosis signals. Crystallography; AP2 COOH-terminala-adaptin appendage domain residues701 to 938 -
EMBO Practical Course on the Crystallization of Macromolecular…
https://www2.mrc-lmb.cam.ac.uk/groups/cartera/download/cv/2020_01_Carter_CV.pdf31 Jan 2020: Nat Cell Biol. 18: 1018-24. 11) Schmidt H, Carter AP‡ (2016) Review: Structure and mechanism of the dynein. ... Science. 322: 1691-5. 24) Gennerich A, Carter AP, Reck-Peterson SL, Vale RD‡ (2007) Force-induced bidirectional stepping of cytoplasmic -
nature structural biology . volume 5 number 6 . june 1998
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nsb1998.pdf14 Jan 2002: 24.Chook, Y.M., Gray, J.V., Ke, H. & Lipscomp, W.N. The monofunctional chorismate mutase from Bacillus subtilis: structure determination of chorismate mutase and its complexes with a transition state ... J. Appl. Cryst. 24, 946-950 (1991). 39.Merrit, E.A. -
FigS1
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/SchmidBetaPaper/BetaHubSup.pdf7 Sep 2006: AP180(27), Eps15(6). Dynamin1,2,3 (89,30,24), Amph1(24). Eps15R(52), AP180(5), Dynamin1(1). Amph1(15), Eps15R(2). Amph2(40), Epsin1(1). -
Cryo-EM Model-Buildingwith Modern Coot Paul Emsley@ Ben Gurion…
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/coot-2020-nov-slac.pdf10 Nov 2020: Slide 23. Slide 24. Slide 25. Slide 26. Slide 27. Slide 28. -
Organization of spines on the dendritesof Purkinje cellsJohn O’Brien…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnas06.pdf25 Jul 2006: 2004) Nat. Rev. 5, 24 –34.4. Matus, A. (2000) Science 290, 754 –758.5. ... T., Wong, R. O. L. & Lichtman, J. W. (2000) Neuron 27, 219 –225.24. -
LETTERS A bacterial dynamin-like proteinHarry H. Low1 & Jan ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nature%20BDLP%202006.pdf28 Nov 2006: Diffraction data 3.0 Å, all data 3.1 Å, all dataR factor, Rfree 0.24 (0.34), 0.28 (0.39) 0.22 (0.35), 0.27 (0.36)B ... Nature 440, 101–104 (2006). 24. Hales, K. G. & Fuller, M. -
Structure and superorganization of acetylcholinereceptor–rapsyn…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnas13.pdf3 Jul 2013: 20%of the receptors showed restricted diffusion in small domains of50 nm, whereas the majority of the receptors were immobile (24).Confocal microscopy of overexpressed rapsyn in nonmuscle. ... The cross-linking of receptors by rapsyn,therefore, seems to -
Filament structure and subcellular organization of the bacterial…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Liu_PNAS_crescentin_2024.pdf28 Apr 2024: This is reminiscent of eukaryotic intermediate filaments (IFs), which are also known to be non- polar (21–24). ... mass spectrometry anal-yses of IF protein oligomers, and recent cryo- EM analyses (21–24,. -
MD1-116-117 Morpheus III-Morpheus III HT-96
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/LMB22.pdf13 Oct 2020: 3 8.5 30 % Precipitant Mix 21-23 B11 1.5 % Vitamins mix 0.1 M Buffer System 3 8.5 30 % Precipitant Mix 31-24 B12 1.5 % Vitamins mix ... 8.5 30 % Precipitant Mix 32-24 F12 0.6 % Antibiotics mix 0.1 M Buffer System 3 8.5 37.5 % Precipitant Mix 42-25 G1 1.2 -
KS_2012_A5_Meeting.indd
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/conferences/2012_A5_Save_the_Date.pdf19 Sep 2011: Late-Breaking Abstract:October 24, 2011. Early Registration:November 21, 2011. Deadlines. late-breaking research • cross-disciplinary collaboration • networking opportunities • peer-reviewed meeting programs. -
se060101051p
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/ford01.pdf3 Dec 2004: The COOH-termi-nal domains of these proteins contain clath-rin-binding motifs (3, 24 ), as well as Asp-Pro-Phe (DPF)-like a- and b-adaptin–. ... De Camilli, A. T.Brunger, J. Cell Biol. 149, 537 (2000). 24. -
1202.tif
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb84.pdf16 Jun 2006: Science (Wash. DC). 208:1454-1457. 24. Ross, M. J., M. W. Klymkowsky, D. ... J. Ultrastruct. R e s 24:454-564. 27. Ziogsheim, H.-P., D.-C. Neugehauer, F. -
doi:10.1016/j.jmb.2004.05.031
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/zapa%202004.pdf16 Aug 2004: In vitro, FtsZ isextremely versatile, forming protofilaments, thickfilaments, sheets,21,22 helical tubes23,24 and asters.25. ... FEMS Microbiol. Rev. 24,531–548. 13. Rothfield, L., Justice, S. & Garcia-Lara, J.(1999). -
www.landesbioscience.com Autophagy 805 Autophagy 6:6, 805-807; August …
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/20574157.pdf2 Aug 2010: Submitted: 05/24/10. Revised: 06/03/10. Accepted: 06/03/10. Previously published online: www.landesbioscience.com/journals/autophagy/article/12577Correspondence to: Roger L. -
validation-tutorial.dvi
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/tutorial/validation-tutorial.pdf24 Oct 2017: Tutorial: Validation with Coot. CSHL 2017. October 24, 2017. 1 Preamble. -
DOI: 10.1126/science.1136985 , 120 (2007); 316Science et al.Maria C.…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Alonso_Science.pdf6 Apr 2007: 151, 1081 (2000).24. R. B. Case, S. Rice, C. L. Hart, B. -
TBS may template.qxd
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/EMreview.pdf3 May 2002: In each of thesecases, purified soluble factors and syntheticliposomes are sufficient to reconstitute coatformation [21–24]. ... Proc. Natl. Acad. Sci. U. S. A. 95,11199–11204. 24 Matsuoka, K.
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