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A Large Particle Associated with the Perimeter of the ...
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb82.pdf5 Jul 2010: Best preserved porecomplexes show evidence of 16- and 24-fold harmonics inaddition to the basic eightfold harmonic (Fig. ... J. CellBiol. 52:338-354. 24. Paine, P. L. , L, C. Moore, and S. -
Chapter 20 In Vitro Dissection of Protein Translocationinto the ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/62_Sharma_AS_MMB_2010.pdf30 Apr 2010: Chapter 20. In Vitro Dissection of Protein Translocationinto the Mammalian Endoplasmic Reticulum. Ajay Sharma, Malaiyalam Mariappan, Suhila Appathurai,and Ramanujan S. Hegde. Abstract. In eukaryotic cells, roughly one-fourth of all mRNAs code for -
Crystal Structures of Bacillus subtilis Lon Protease
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/londumanjmb2010.pdf20 Jul 2010: electron microscopy data show that negativelystained images of E. coli Lon particles appearhexameric,24 although surprisingly, electron cryo-microscopy images of S. -
A positively charged channel within the Smc1/Smc3 hinge required for…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embosmcjingekurze2010.pdf1 Dec 2010: Smc1DDSmc3AAA. Smc1Smc3AAA. Kd = 22 nM Kd = 29 nM Kd = 24 nM. ... The Kd for Smc1DD and Smc3AAA hingedomain association is 29 nM (middle panel) and 24 nM for Smc1 wild-type and Smc3AAA (right panel). -
Doc2b Is a High-Affinity Ca2+ Sensor forSpontaneous Neurotransmitter…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/GroffenScience2010.pdf30 Apr 2010: S1) (24). Doc2b/ mice did not expressthe remaining exons and lacked Doc2b immuno-reactivity. ... Neurosci. 24, 1226 (2004).16. A. Maximov, O. H. Shin, X. Liu, T. -
Quality and quantity control at the endoplasmic reticulum
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/65_Hegde_RS_COCB_2010.pdf7 Jun 2010: EMBO J 2005,24:149-159. 55. Bays NW, Gardner RG, Seelig LP, Joazeiro CA, Hampton RY:Hrd1p/Der3p is a membrane-anchored ubiquitin ligaserequired for ER-associated degradation. ... EMBO J 2005, 24:3917-3926. 57. Isaacson MK, Ploegh HL: Ubiquitination, -
Many bacterial plasmids are maintained at low copy number ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nrmicro2425.pdf14 Sep 2010: This relationship was confirmed by the crystal structure of ParM, which revealed a fold and domain arrangement very similar to that of globular actin (G-actin)24 (FIG. ... 24) 166.2 (REF. 89). Kinetic parameters. Critical concentration (ATP) 2.3 μM18 -
Roles of Amphipathic Helices and the Bin/Amphiphysin/Rvs(BAR) Domain…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Jao_JBC2010.pdf4 Oct 2010: Distances 20Å were investigated using four-pulse DEER (24–27), whereasshorter distances were investigated using continuous wave EPR(28, 29) (see “Experimental Procedures”).The DEER measurements are illustrated with the example ... Mutant -
Bacterial actin MreB assembles in complex with cell shape protein RodZ
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embo2010RodZMreB.pdf17 Feb 2010: The crystals were cryoprotected with mother liquorcontaining 24.8% PEG400 and flash frozen in liquid nitrogen. ... The plate was incubated at 301Cand imaged after 24 h. DrodZ complementation assays and microscopy. -
Features critical for membrane binding revealed by DivIVA crystal…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/emboj201099a.pdf20 May 2010: 21.7) 24.1 (ano. 14.1) 3.8 4.3 (ano. 2.5) 2.8. Phasing Se SAD Ta SAD MR I SAD MRNumber of sites 6 5 8Mean FOM
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