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15 Sep 2020: For correspondence:. jyl@mrc-lmb.cam.ac.uk (JL);. ashley.nasmyth@bioch.ox.ac.uk. (KAN). †These authors contributed. equally to this work. Competing interests: The. authors declare that no. competing interests exist. Funding: See page 33. Received:

1 Jul 2011: Int JBiochem Cell Biol 41:323–329[3] Aylett CH, Wang Q, Michie KA et al. ... Cell 115:705–713[12] Michie KA, Löwe J (2006) Dynamic filaments of the bac-terial cytoskeleton.

16 Jul 2003: smaller ka) but prolonged its membrane residence by3-fold (i.e. smaller kd). ... Proteins ka kd kdFold increase. in Kda. M 1 s 1 s 1 M.

11 Nov 2010: Nature 413:39–44. 3. Aylett CHS, Wang Q, Michie KA, Amos LA, Löwe J(2010) Filament structure of bacterial tubulin homo-logue TubZ.

12 Oct 2002: The enhanced membrane afnity of the p47phox-PXdomain due to 3 mol% PtdIns(3,4)P2 is almost entirely dueto a decrease in the kd, with the ka remaining nearlyconstant. ... Phosphoinositide ka (M1 s1) kd (s. 1) Kd (M) Fold increase in Kda.

25 Nov 2012: Full lipid-binding curves used tocalculate Ka values are in Fig. S7. ... B) Protein–lipid FRET assays were per-formed with 5% PIP2 vesicles, and Ka was calculated for both 5 μM pY.

3 Sep 2014: Citation: Michie KA, Boysen A, Low HH, Møller-Jensen J, Löwe J (2014) LeoA, B and C from Enterotoxigenic Escherichia coli (ETEC) Are Bacterial Dynamins. ... 24. Datsenko KA, Wanner BL (2000) One-step inactivation of chromosomal genes.

19 Apr 2002: tide linker had little or no effect on its ability to bindstate affinity measurements to estimate on rate (ka), offrate (kd), and affinity (KA ka/kd) constants by measuring Scc1 (Figure ... dissociation rate constants (ka and kd, respectively) are

23 Nov 2004: fitted to the data using the ORIGIN program supplied by themanufacturer yielding the stoichiometry N, the binary equilibrium constant Ka (= Kd-1)and the enthalpy of binding. ... Peptide binding to a-adaptin wild-type appendagePeptide Sequence Kd(mM)

17 Jun 2005: XR r 1)2 4XR)1/2)) V, with XR = [LT]/[MT] and r = 1/KA[MT](Wiseman et al., 1989). ... This fit optimizes parameters of stoichiom-etry (N), association constant (KA), and the enthalpy of binding (H).The dissociation constant (KD) is the inverse of KA.

3 Jul 2013: J Biol Chem 276(27):24911–24917. 6. Huebsch KA, Maimone MM (2003) Rapsyn-mediated clustering of acetylcholine re-ceptor subunits requires the major cytoplasmic loop of the receptor subunits.J

28 Nov 2008: Titration curves were fitted to the data using the ORIGIN program (MicroCal Inc.) which yielded the stoichiometry (1), the binary association constant Ka (=Kd -1) and the enthalpy of binding. ... The entropy of binding (S) was calculated from the

23 Dec 2013: Chem. 286:11756 –11764. 48. Burton BM, Marquis KA, Sullivan NL, Rapoport TA, Rudner DZ.

14 Nov 2021: aerolysin family protein. Nat Commun 7, 11293. 23 Zheng Shawn Q, Palovcak E, Armache J-P, Verba KA,.

27 Apr 2015: Mol Microbiol 67(4):881–890. 15. Aylett CH, Wang Q, Michie KA, Amos LA, Löwe J (2010) Filament structure of bacterialtubulin homologue TubZ.

9 Jan 2003: Titration curves were fitted to the data using the ORIGINprogram (MicroCal, Inc.) yielding the stoichiometry N, the binary equilib-rium constant KA ( KD 1) and the enthalpy of binding.

15 Feb 2007: J Neurochem 67:1735–1743. 25. Roos J, DiGregorio PJ, Yeromin AV, Ohlsen K, Lioudyno M,Zhang S, Safrina O, Kozak JA, Wagner SL, Cahalan MD,Velicelebi G, Stauderman KA (2005) ... Biochem J289 (Pt 1):71–79. 36. Zhang SL, Yu Y, Roos J, Kozak JA, Deerinck

17 Sep 2008: J Bacteriol 2006;188:469–76. ichie KA, Löwe J. Dynamic filaments of the bacterial cytoskeleton.

14 Jul 2021: For correspondence:. jyl@mrc-lmb.cam.ac.uk (JL);. ashley.nasmyth@bioch.ox.ac.uk. (KAN). †These authors contributed. equally to this work. Competing interests: The. authors declare that no. competing interests exist. Funding: See page 28. Received:

21 Mar 2003: 740 Research Paper. Calcium triggers calcineurin-dependent synaptic vesicle recyclingin mammalian nerve terminalsBruno Marks and Harvey T. McMahon. Background: Following exocytosis at the synapse, synaptic vesiclecomponents are recovered by