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Carter lab dynactin prep from frozen porcine brains, using ...
https://www2.mrc-lmb.cam.ac.uk/groups/cartera/download/2020_Carter_dynactin_brain_prep.docx28 Feb 2020: Overnight, wash the G4000 from storage in 20% EtOH to water (2 CV). ... Stored in. SP-sepharose. 250. 0.3. 4. 20% EtOH. MonoQ 16/10. 20. -
Sub ForwardPrimer() ' ' ForwardPrimer Macro ' '…
https://www2.mrc-lmb.cam.ac.uk/groups/cartera/download/resources/computer/word_macros/UsefulMacros.docx25 Jan 2019: Sub ForwardPrimer(). '. ' ForwardPrimer Macro. '. '. Selection.MoveRight Unit:=wdCharacter, Count:=20, Extend:=wdExtend. ... Selection.Font.Underline = wdUnderlineSingle. Selection.MoveLeft Unit:=wdCharacter, Count:=1. End Sub. Sub ReversePrimer(). '. ' -
Carter lab insect cell ZZ-tag prep (2020) Buffers Wash ...
https://www2.mrc-lmb.cam.ac.uk/groups/cartera/download/resources/protocols/2020_Carter_Sf9_dynein_prep.docx24 Jan 2020: Gel-filtration on AKTAmicro. -Column is stored in water 0.05% NaN3. If stored in 20% ethanol, first wash into water before washing into buffer. ... Remove column and replace caps on either end. -Put inlet A1 into 20% ethanol, pumpwash. -
31/10/2018 Carter lab TIRF microscopy Single Molecule Assay 5x ...
https://www2.mrc-lmb.cam.ac.uk/groups/cartera/download/resources/protocols/2020_Carter_TIRF_protocol.docx28 Jan 2020: 15 mg Glucose Oxidase 2mg Catalase (both in -20) into 250 μL DLB. ... Don’t pipette up and down. Could use a cut 20 μL tip for this. -
Madeline Lancaster Fixation, cryosectioning and immunostaining brain…
https://www2.mrc-lmb.cam.ac.uk/groups/lancaster/download/Analyzing-brain-organoids.docx1 Aug 2019: Fix at 4C for 15-20 min. 2. Wash 3X for 10 min in PBS. -
Madeline Lancaster Cerebral organoids Version 2.1 from feeder-free…
https://www2.mrc-lmb.cam.ac.uk/groups/lancaster/download/Cerebral-organoids-2-1-FF.docx17 Apr 2023: Matrigel droplet. Spinning bioreactor/shaker. Day 5. hES media. Day 20 Day 40. ... Matrigel droplet. Spinning bioreactor/shaker. Day 5. hES media. Day 20. Day 40. -
Madeline Lancaster Generation of cerebral organoids using the SCT ...
https://www2.mrc-lmb.cam.ac.uk/groups/lancaster/download/Cerebral-organoids-kit.docx17 Apr 2023: c. Place parafilm sheet in a dish in the 37 incubator for 20 min to allow matrigel to polymerize. -
Madeline A. Lancaster Culturing feeder-dependent human iPS/ES cells…
https://www2.mrc-lmb.cam.ac.uk/groups/lancaster/download/Culturing-human-ES-and-iPS-cells.docx21 Mar 2014: 20 ng/ml bFGF (Add just before use). MEF Media (500 ml) – Store at 4C up to 2 weeks. ... 20 mg Dispase. 40 ml DMEM/F-12. Filtered using 0.22 um filter. -
Madeline A. Lancaster Embedding and sectioning tissues in gelatin ...
https://www2.mrc-lmb.cam.ac.uk/groups/lancaster/download/Gelatin-embedding.docx29 Oct 2012: Fix at 4C for 15-20 min. 2. Wash 3X for 10 min in PBS. -
Madeline Lancaster Cerebral organoids Version 2.0 Materials ·…
https://www2.mrc-lmb.cam.ac.uk/groups/lancaster/download/resources/Cerebral-organoids-2-0.docx25 May 2015: Day 5. hES media. Day 20 Day 40. dissolved Matrigel. Day 13.
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