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  2. Meet the LMBees - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/meet-the-lmbees/
    Thumbnail for Meet the LMBees - MRC Laboratory of Molecular Biology 3 Jul 2018: The LMB has recently become home to 16,000 honey bees, representing two nucs (also known as nucleus colonies) which will form the basis of two new hives.
  3. How does the replisome respond to DNA damage? - MRC Laboratory of…

    https://www2.mrc-lmb.cam.ac.uk/how-does-the-replisome-respond-to-dna-damage/
    Thumbnail for How does the replisome respond to DNA damage? - MRC Laboratory of Molecular Biology 1 Jun 2018: Work by Joe Yeeles’ group in the LMB’s PNAC Division has for the first time revealed the earliest responses when the eukaryotic DNA replication machinery,
  4. Uncovering how alcohol-derived metabolites damage the genome of stem…

    https://www2.mrc-lmb.cam.ac.uk/uncovering-alcohol-derived-metabolites-damage-genome-stem-cells/
    Thumbnail for Uncovering how alcohol-derived metabolites damage the genome of stem cells - MRC Laboratory of Molecular Biology 4 Jan 2018: Previous work from KJ Patel’s group in the LMB’s PNAC Division revealed that aldehydes – such as acetaldehyde, a by-product of alcohol metabolism – can
  5. Lori Passmore and Felix Randow elected to EMBO membership - MRC…

    https://www2.mrc-lmb.cam.ac.uk/lori-passmore-and-felix-randow-elected-to-embo-membership/
    Thumbnail for Lori Passmore and Felix Randow elected to EMBO membership - MRC Laboratory of Molecular Biology 14 May 2018: Lori Passmore, group leader in the LMB’s Structural Studies Division, and Felix Randow, group leader in the LMB’s PNAC Division, have been newly elected
  6. Cambridge LIFE LAB project wins place in Europe’s largest public…

    https://www2.mrc-lmb.cam.ac.uk/cambridge-life-lab-project-wins-place-in-europes-largest-public-science-event/
    Thumbnail for Cambridge LIFE LAB project wins place in Europe’s largest public science event - MRC Laboratory of Molecular Biology 11 Jun 2018: Five Cambridge science institutions including the LMB have won a bid to engage the local region with science as part of European Researchers’ Night, the
  7. Twelfth annual LMB CamAWiSE “What Next For Your Career In Science?”…

    https://www2.mrc-lmb.cam.ac.uk/twelfth-annual-lmb-camawise-what-next-for-your-career-in-science-event/
    Thumbnail for Twelfth annual LMB CamAWiSE “What Next For Your Career In Science?” event - MRC Laboratory of Molecular Biology 14 Jun 2018: An audience of LMB staff and visitors from across Cambridge gathered in the LMB’s Max Perutz Lecture Theatre for the annual “What Next For Your Career In
  8. Instinctive and learned responses to smells are controlled by a…

    https://www2.mrc-lmb.cam.ac.uk/instinctive-and-learned-responses-to-smells-are-controlled-by-a-single-brain-circuit-in-flies/
    Thumbnail for Instinctive and learned responses to smells are controlled by a single brain circuit in flies - MRC Laboratory of Molecular Biology 20 Sep 2018: Published on. 20 September, 2018.
  9. Aaron Klug (1926 - 2018) - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/aaron-klug-1926-2018/
    Thumbnail for Aaron Klug (1926 - 2018) - MRC Laboratory of Molecular Biology 21 Nov 2018: Aaron Klug, Director of the LMB from 1986 to 1996, President of The Royal Society from 1995 to 2000 and 1982 Nobel Laureate, died on Tuesday 20.
  10. The LMB art show goes crafty   - MRC Laboratory of Molecular Biology

    https://www2.mrc-lmb.cam.ac.uk/the-lmb-art-show-goes-crafty/
    Thumbnail for The LMB art show goes crafty   - MRC Laboratory of Molecular Biology 3 Aug 2018: Over 20 exhibitors participated, with photography being one of the most popular categories.
  11. ProSHADE: ProSHADE Documentation

    https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/index.html
    1 Nov 2018: On line 20, the path to the standard C++ library is required. ... $ proshade -O -f. /2A2Q_T_dom_2_rotated_translated.map -f. /2A2Q_T_dom_2.pdb --clearMap res.pdb --cellBorderSpace 20.
  12. Richard Henderson made Companion of Honour - MRC Laboratory of…

    https://www2.mrc-lmb.cam.ac.uk/richard-henderson-made-companion-of-honour/
    Thumbnail for Richard Henderson made Companion of Honour - MRC Laboratory of Molecular Biology 11 Jun 2018: For the last 20 years, he has been working to improve the methodology of single particle electron cryomicroscopy (cryo-EM), which has recently reached the stage where it is possible to
  13. Making a cell-based factory for polymer synthesis - MRC Laboratory of …

    https://www2.mrc-lmb.cam.ac.uk/making-a-cell-based-factory-for-polymer-synthesis/
    Thumbnail for Making a cell-based factory for polymer synthesis - MRC Laboratory of Molecular Biology 6 Dec 2018: However, proteins are more complex than most synthetic polymers because they are composed of 20 different compounds (amino acids) strung together in defined order. ... Jason Chin’s group has a long-term interest in creating cell-based factories that
  14. How cells selectively enhance gene expression in response to stress - …

    https://www2.mrc-lmb.cam.ac.uk/how-cells-selectively-enhance-gene-expression-in-response-to-stress/
    Thumbnail for How cells selectively enhance gene expression in response to stress - MRC Laboratory of Molecular Biology 5 Sep 2018: As these 61 different codons encode for 20 different amino acids, most amino acids correspond to more than one codon.
  15. Two new group leaders at the LMB: Chris Russo and Gerry Crossan - MRC …

    https://www2.mrc-lmb.cam.ac.uk/two-new-group-leaders-at-the-lmb-chris-russo-and-gerry-crossan/
    Thumbnail for Two new group leaders at the LMB: Chris Russo and Gerry Crossan - MRC Laboratory of Molecular Biology 20 Jul 2018: Published on. 20 July, 2018. The LMB is delighted to announce that Chris Russo and Gerry Crossan have progressed from Independent Investigator Scientist positions to become Group Leaders at the LMB.
  16. How neuropeptide signalling controls sensitisation in response to…

    https://www2.mrc-lmb.cam.ac.uk/how-neuropeptide-signalling-controls-sensitisation-in-response-to-touch-in-c-elegans/
    Thumbnail for How neuropeptide signalling controls sensitisation in response to touch in C. elegans - MRC Laboratory of Molecular Biology 24 Aug 2018: In this study, the researchers found that the neuropeptide FLP-20 is released by touch-sensing neurons and leads to sensitisation of chemical-sensing neurons and increased movement speed. ... Yee Lian and Isabel showed that the neuropeptide FLP-20 acts
  17. Electron cryo-microscopy reveals near-atomic resolution structure of…

    https://www2.mrc-lmb.cam.ac.uk/cryo-electron-microscopy-reveals-near-atomic-resolution-structure-of-the-prespliceosome/
    Thumbnail for Electron cryo-microscopy reveals near-atomic resolution structure of the prespliceosome - MRC Laboratory of Molecular Biology 12 Jul 2018: The human genome contains approximately 20,000 genes, which when transcribed produce precursor messenger RNA (pre-mRNA) consisting of coding sequences (exons) and non-coding sequences (introns).
  18. Children enjoy an afternoon of discussing science books and hands-on…

    https://www2.mrc-lmb.cam.ac.uk/children-enjoy-an-afternoon-of-discussing-science-books-and-hands-on-experiments-with-lmb-postdocs/
    Thumbnail for Children enjoy an afternoon of discussing science books and hands-on experiments with LMB postdocs - MRC Laboratory of Molecular Biology 20 Sep 2018: Published on. 20 September, 2018.
  19. LMB researchers are taking part in LifeLab with events around…

    https://www2.mrc-lmb.cam.ac.uk/lmb-researchers-are-taking-part-in-lifelab-with-events-around-cambridgeshire-on-friday-28th-and-saturday-29th-september/
    Thumbnail for LMB researchers are taking part in LifeLab with events around Cambridgeshire on Friday 28th and Saturday 29th September - MRC Laboratory of Molecular Biology 20 Sep 2018: Published on. 20 September, 2018. LMB researchers are helping to transform parts of Cambridgeshire into centres of discovery with hands-on, family-friendly activities in shopping centres, cafes and public spaces.
  20. tutorial.dvi

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/newt.pdf
    18 Jun 2018: 20. Figure 5: Coot’s Go To Atom Window (it doesn’t look exactly like this anymore). •
  21. Publications

    https://www2.mrc-lmb.cam.ac.uk/groups/ms/Publications.html
    15 Oct 2018: 2010. Mammalian mRNA export requires GANP. Curr. Biol. 20:25-31. A.Giesecke & M.Stewart. ... 1.25 A resolution structure of an RNA 20-mer that binds to the TREX2 complex.
  22. OUTLINE OF RESEARCH

    https://www2.mrc-lmb.cam.ac.uk/groups/ms/Research.html
    15 Jan 2018: Structure, 20:1007-1018. C.Sun, G.Fu, D.Ciziene, M.Stewart & S.M.Musser. (2013). Choreography of Importin? ... 2010). Mammalian mRNA export requires GANP. Curr. Biol. 20:25-31. A.M.Ellisdon, D.Jani, A.Köhler, E.Hurt & M.Stewart.
  23. mbar-v2

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/mbar-v2.pdf
    31 Aug 2018: Under “Weight Matrix”, set the Refinement Weight to 20. 1.2. Automatic model building will only rarely produce a model which is both complete and correct.
  24. CCP4 Program Suite: acedrg

    https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/acedrg/acedrg.html
    13 Dec 2018: sp3_sp3_112 C27 C26 O3 C25 180.000 10.00 3 UNL sp3_sp3_115 C24 C25 O3 C26 180.000 10.00 3 UNL sp3_sp3_20 C12 C24
  25. Coot: Model-building tools for Molecular Graphics

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/coot-cshl-2018.pdf
    24 Oct 2018: Slide 19. Slide 20. Slide 21. Slide 22. Slide 23. Slide 24.
  26. REFMAC5/ProSMART Tutorial

    https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/tutorials/refmac_tutorial/files/part_4.html
    23 Jun 2018: Show Me This. Set the "Number of refinement cycles" to 20, and untick "Use hydrogens during refinement".
  27. ProSHADE: src/bin/bin.cpp Source File

    https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/d2/db3/bin_8cpp_source.html
    1 Nov 2018: 961 setUp->peakDistanceForReal = 0.20;.
  28. Journal of Visualized Experiments www.jove.com Copyright © 2018…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/JOVE2018.pdf
    19 Apr 2018: Then, add 20 L of deionized water to the main container of the liquid handler. ... After use, flushing with water and then 20% v/v ethanol solution prevents microbial growth.
  29. ProSHADE: src/proshade/ProSHADE_lapack.cpp Source File

    https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/d7/dee/_pro_s_h_a_d_e__lapack_8cpp_source.html
    1 Nov 2018: 1. 20 //============================================ Lapack-E.
  30. Cryo-EM reconstruction of AlfA from Bacillus subtilis reveals the…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/AlfA2018.pdf
    20 Mar 2018: The locus codesfor the ParMRC system that, besides ParM, contains the DNA-binding protein ParR, and a centromeric sequence parC (19, 20).Principles governing plasmid segregation by the ParMRC systemare well ... J Mol Biol269:505–513. 20. Popp D, et al.
  31. Coot: Model-building tools for Molecular Graphics

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/bgu/coot-2018-bgu.pdf
    22 Feb 2018: Slide 20. Slide 21. Slide 22. Slide 23. Slide 24. Slide 25.
  32. Coot Tools for Cryo-EM Paul EmsleyMRC Laboratory of Molecular ...

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/coot-cryoEM-2018-Oct-TF.pdf
    8 Oct 2018: Slide 16. Slide 17. Slide 18. Slide 19. Slide 20. Slide 21.
  33. Interactive Tools forCryo-EM Map Fitting Paul EmsleyMRC Laboratory of …

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/coot-cryoEM-2018-June-PSI.pdf
    19 Jun 2018: Slide 19. Slide 20. Slide 21. Slide 22. Slide 23. Slide 24.
  34. Manipulating Ligands Using Coot with a bit of carbohydrates ...

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/bgu/coot-feb-2018-ligands-carbohydrate-bgu.pdf
    22 Feb 2018: 60.005 20.000 1 ADP var_2 PA O3A PB O1B 59.979 20.000 1 ADP var_3 O2A PA "O5'" "C5'" -59.942 20.000 1 ADP ... 20.000 1 ADP var_7 "C5'" "C4'" "C3'" "C2'" -150.000 20.000 3.
  35. Coot Tools for Cryo-EM Paul EmsleyMRC Laboratory of Molecular ...

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/coot-cryoEM-2018-Aug-Stockholm.pdf
    15 Aug 2018: Slide 17. Slide 18. Slide 19. Slide 20. Slide 21. Slide 22.
  36. mbar-v2

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/tutorial/model-building-and-refinement-v2.pdf
    31 Aug 2018: Under “Weight Matrix”, set the Refinement Weight to 20. 1.2. Automatic model building will only rarely produce a model which is both complete and correct.
  37. ProSHADE: src/proshade/ProSHADE_internal.h Source File

    https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/db/db2/_pro_s_h_a_d_e__internal_8h_source.html
    1 Nov 2018: 20 #include "ProSHADE_version.h".
  38. Prokaryotic cytoskeletons: protein filaments organizing small cells

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nrmicro.2017.153.pdf
    18 Jan 2018: Nature Reviews Microbiology, (2018). doi:10.1038/nrmicro.2017.153
  39. ProSHADE: src/proshade/ProSHADE_clipper.cpp Source File

    https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/d9/d66/_pro_s_h_a_d_e__clipper_8cpp_source.html
    1 Nov 2018: 240 double ecsHlp = std::floor ( std::max ( 20.0, ( this->_maxMapRange / 4.0 ) ) );. 241. ... 764 while ( std::floor ( this->_maxMapRange / this->_shellSpacing ) < 20 ). 765 {. 766 this->_shellSpacing /= 2.0;.
  40. ProSHADE: src/proshade/ProSHADE_internal.cpp Source File

    https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/d9/dc4/_pro_s_h_a_d_e__internal_8cpp_source.html
    1 Nov 2018: 1. 20 //============================================ Clipper.
  41. ProSHADE: src/proshade/ProSHADE_SO3transform.cpp Source File

    https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/d7/db2/_pro_s_h_a_d_e___s_o3transform_8cpp_source.html
    1 Nov 2018: ProSHADE 0.6.5 (NOV 2018). Protein Shape Descriptors and Symmetry Detection. ProSHADE_SO3transform.cpp. Go to the documentation of this file.. 1. 21 //============================================ FFTW3 SOFT. 22 #ifdef __cplusplus. 23 extern "C" {.
  42. The ER membrane protein complex is a transmembrane domain insertase

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017c.pdf
    27 Jan 2018: TA proteins were translated for 20 minutes at 32 C unless otherwise indicated. ... The insertion reaction proceeded at 32 C for 20 minutes unless otherwise stated.
  43. ProSHADE: src/proshade/ProSHADE_spharmonickit.cpp Source File

    https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/d8/dd5/_pro_s_h_a_d_e__spharmonickit_8cpp_source.html
    1 Nov 2018: 1. 20 //============================================ FFTW3 SOFT.
  44. ProSHADE: src/proshade/ProSHADE_descriptors.cpp Source File

    https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/df/d91/_pro_s_h_a_d_e__descriptors_8cpp_source.html
    1 Nov 2018: 820 //======================== Integrate over all radii using 20-point Gauss-Legendre.
  45. 15211199233993 1..45

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MreBHussain2018.pdf
    15 Mar 2018: B) Confined cells induced at 3 mM. xylose in 20 mM Mg2+ progressively swell upon escaping confinement into free culture. ... Bottom) BRB785 cells in 20 mM Mg2+ were depleted of Pbp2a by growth in 0 mM IPTG.
  46. Morpheus stock solutions poster portrait A2

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/PresentationsOther/Morpheus_stock_solutions_poster.pdf
    1 Jun 2018: Buffer system 3 MORPHEUS I & III MD2-100-102 Two stocks: 1 M bicine (acid, pKa 8.4) and 1 M Trizma-base (pKa 8.20).

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