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14 Dec 2006: Christien explains. how it really works.

12 Jun 2006: but not for long.

3 Aug 2006: Gamze getting the knack.

27 Feb 2006: Sterilise (heat B 98C, 20 min 121.5C). This is not OK for sugars. ... When up to speed (15K) start culture pump at speed '10' (may work at 20 as well), adjust so that eflux is clear (after debubbling).

29 Sep 2006: Binds accessory proteins via appendage domain. Cargo recognition. mu1 (A/B). Binds Yxx(bulky hydrophobic) cargo motifs [20]. ... J Biol Chem, 2000. 275(29): p. 21862-9. 20. Ohno, H., et al., Interaction of tyrosine-based sorting signals with

28 Mar 2006: Add to M9 prior to experiment (per litre):15NH4Cl 1g (can dissolve in 10 mL H2O and filter sterilise into media)20% glucose 20 mLvitamins 1 mLtrace elements 10 mLMgSO4 (1M) ... 10 mL of 20% arabinose for BAI cells). Purification:As normal, however

7 Sep 2006: 15%out of 132 residues. 22%out of 114 residues. 20%out of 237. ... residues. 20%out of 128 residues. 128 residues in total. hβ4-appendage. 237 residues in total.

4 Jan 2006: ratio. n (D. 280). 0 10 20. 0.00. 0.05. 0.10. 0.15. ... n (D. 280). 0 5 10. 0.00. 0.10. 0.20. s (Svedbergs).

25 Aug 2006: The thickness of the. sections is 15 Å in (A-F), and 20 Å in (G-I). ... was reported to rotate clockwise by 20 in the ADP state compared with the AMPPNP state.

8 Jun 2006: Readings were performed at 20 C using an average of 5 scans with buffer subtracted. ... uals. 6.00 10 20Concentration (µM). 0. 20. 40. 60. 80 (k.

28 Sep 2006: The lysate buffer consisted of 50 mM Tris-Cl, 1.25 M sodium chloride, final pH 8.0, the wash buffer of 50 mM Tris-Cl, 500 mM sodium chloride, 20% ... For the phylogenetic tree, 10-20 sequences related to the different classes of dynamin-related proteins

28 Nov 2006: 100 120. kDa 220. Nosto. c p. ly. sate. M. 0 0 0.2 0.4 0.6 0.8 1.0 1.2 40 80 60 20. ... 60. 40. 0. 20. 80. WT K82A mutant. 0.2 0.4 0.6 0.8 1.0 1.2.

19 Apr 2006: 2.2. Linear amplification reaction. 2–20 nM of PCR product was used in a linear amplification reaction with 6–400 pM ofpHis17 (B. ... The protein was purified over a Ni-NTA spin column under native conditions (lanes 3 and 5) and separated on a 10–20

4 Jan 2006: fatty acid-free BSA and 0.01% Triton X-100 with substrate concentrations (20–200 mM LPA) at 25 8C and 37 8C with 14C-labelled oleoyl-CoA (Amersham) at aspecific ... Liposomes made from Folch brain lipid extract at0.2 mg ml21 in 20 mM HEPES pH 7.4, 150

12 Oct 2006: Radiolabeled 20-bp fragmentsthat lacked KOPS (nKOPS) or that contained a single KOPS (sKOPS). ... 50 l culture. NMR buffer was 20 mM potassium phosphate and 150 mM.

8 Jun 2006: analytical ultracentrifuge with an An60-Ti rotor, with 39, 78 and 156 µM in 150 mM NaCl, 20. ... at 40 and 150 µM protein in 150 mM NaCl, 20 mM HEPES pH 7.4, 2 mM DTT unless.

28 Nov 2006: 2000) J Biol Chem 275:36062–36066.20. Jeon KI, Byun MS, Jue DM (2003) Exp Mol Med 35:61– 66.21. ... Twenty-four hours after transfection, cells were serum-starved for 16 h and then stimulated with 20 ng/ml TNF-α.

16 Jun 2006: The amino acid sequences deduced from recent cDNA sequences (7, 10, 19, 20) indicate molecular weights of 50,000 (a), 54,000 (/), 56,000 (30, and 58,000 (6), and show ... Nature (Lond.). 299:793-797. 20. Noda, M., H. Takahashi, T. Tanabe, M.

25 Jul 2006: The fish dendrites are straighter and less branched than theothers, but some weaver dendrites are also straight over lengths of 20 –30! ... mM NaCl"10 mM KCl"2 mM CaCl2"1 mMMgCl2"2 mM NaHCO3"4 mM Hepes, pH 7.4, supplementedwith Medium 199 (Sigma) in a

26 Jan 2006: Dialyze into 150 mM NaCl, 20 mMHEPES pH 7.4, 0.4 mM DTT for use. ... 1998), dialyzed into HKMbuffer, and centrifuged for 20 min at 100,000g immediately prior to useto remove aggregates.