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The Jour nal o f Cel l Bio logy ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/epsinR.pdf9 Jan 2003: 20, 2003 213–222http://www.jcb.org/cgi/doi/10.1083/jcb.200208023. ... All GST fu-sion proteins were purified from bacterial extracts by incubation with glu-tathione-Sepharose beads, followed by extensive washing with 20 mMHepes, pH 7.4, 150 mM NaCl, -
The Journal of Cell Biology © The Rockefeller University ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsinR/sedequil.pdf16 Jan 2003: The Journal of Cell Biology The Rockefeller University Press • 0021-9525Volume 160 January 20, 2003http://www.jcb.org/cgi/doi/10.1083/jcb.200208023. -
The Journal of Cell Biology © The Rockefeller University ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/sedequil.pdf16 Jan 2003: The Journal of Cell Biology The Rockefeller University Press • 0021-9525Volume 160 January 20, 2003http://www.jcb.org/cgi/doi/10.1083/jcb.200208023. -
The Jour nal o f Cel l Bio logy ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/24_Fons_RD_JCB_2003.pdf6 Feb 2003: factor inthe same proteoliposomes was only. 15–20% of the control,raising the possibility that its transport may require stimula-tory factor(s) not needed by Prl. ... Only after the synthesis of an additional 20 resi-dues did the PrP–Prl intermediate -
x16412u141
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/MaoAP180-2001.pdf17 Mar 2003: vesicle endocytosis. J. Neurosci. 20, 8667–8676. Ye, W., and Lafer, E.M. ... I. Developmental expres-sion and cellular localization of the F1-20 protein and mRNA. -
088542u047
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Owen_McMahon1999.pdf17 Mar 2003: presence of 20 ml 50% slurry of glutathione-following induction with 0.2 mM IPTG. ... Cell Biol. 20, 313–332.pathway in viral infection. EMBO J. 17, 4585–4593. -
x15959u064
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Reim_Brose2001.pdf17 Mar 2003: controls, as well as release phase while the corresponding value in controlCPX I/II double mutant (2/2, 2/2; B) synapses (20,000-fold magnifi-. ... initial synaptic release probability is reduced in these concentrations of 0.5–20 mM, while the Mg21 -
Bb9e55.qxd
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Vallis_McMahon1999.pdf21 Mar 2003: Dynamin GTPase assay and sedimentation assayThese assays have been described previously [20,27] and furtherdetails are given in the Supplementary material. ... Multimerised dynamin was then sedimented by ultracentrifuga-tion at 80,000 g for 20 min. -
554 Research Paper Inhibition of receptor-mediated endocytosis by the …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/wigge_McMahon1997.pdf21 Mar 2003: COS cells. (a) (c). (b) (d)Extract incubated with GST–α-adaptin. over 20 cells quantitated (Figure 4a–c) and the blockadewas rescued by coexpression of dynamin. ... Although we have not demonstrated a lack of dynaminrecruitment to coated pits per se, -
740 Research Paper Calcium triggers calcineurin-dependent synaptic…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Marks_McMahon1998.pdf21 Mar 2003: whereas glutamate release was essentially unaffected (96 9% ofmaximal; n = 3) in the presence of 20 µM cyclosporin A. ... Fol-lowing two washes, the synaptosomes were spun briefly a third timeand resuspended in lysis buffer (20 mM Hepes).
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