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2 Feb 2004: J Mol Biol. 1993 Jan 5;229(1):105-24. The protein is expressed in any E.

23 Nov 2004: Quantitativerepresentations of co-sedimentation assay data of GST-ENTH domains from (D) epsin and (E)AP180 (24) with 0.5% of the indicated phospholipids in the liposomes. ... X-ray Crystallography and NMR (Yale Univ. Press,New Haven, CT, 1993). 24.

3 Dec 2004: 24). Thus our experiments point to a pioneering role forepsin in vesicle budding as it provides both a driving force and a linkbetween membrane invagination, clathrin polymerization and AP2complex recruitment. ... beta 2-adaptin appendage domain. EMBO J.

30 Jan 2004: recruit clathrin to membranes. Mammalian am-phiphysin2 has several clathrin-binding sites (24–26), and we found that the wild-type, but notmut1, efficiently recruited clathrin to liposomes(fig. ... It is interesting to note that thestructure of the

16 Aug 2004: In vitro, FtsZ isextremely versatile, forming protofilaments, thickfilaments, sheets,21,22 helical tubes23,24 and asters.25. ... FEMS Microbiol. Rev. 24,531–548. 13. Rothfield, L., Justice, S. & Garcia-Lara, J.(1999).

3 Dec 2004: The COOH-termi-nal domains of these proteins contain clath-rin-binding motifs (3, 24 ), as well as Asp-Pro-Phe (DPF)-like a- and b-adaptin–. ... De Camilli, A. T.Brunger, J. Cell Biol. 149, 537 (2000). 24.

30 Jun 2004: Saturation of the DNA occurs at an approximate equimolar concentration of 24 bp binding sites to dimeric Spo0J (full length). ... 1C). It is possible to gelfiltrate a stable complex of full-length Spo0J with 16–24 bpoligonucleotides of.

3 Dec 2004: The COOH-termi-nal domains of these proteins contain clath-rin-binding motifs (3, 24 ), as well as Asp-Pro-Phe (DPF)-like a- and b-adaptin–. ... De Camilli, A. T.Brunger, J. Cell Biol. 149, 537 (2000). 24.

3 Dec 2004: 24). Thus our experiments point to a pioneering role forepsin in vesicle budding as it provides both a driving force and a linkbetween membrane invagination, clathrin polymerization and AP2complex recruitment. ... beta 2-adaptin appendage domain. EMBO J.

23 Nov 2004: coatedvesicles. The COPII membrane-associated sec23/24 com-plex has an intrinsic curvature like that found in COPIIvesicles and thus by polymerisation this curvature will befavoured [36]. ... with Sec23/24p in vitro. Stabilises Sec23/24 complex and.

1 May 2004: 2004.Structural/functional homology betweenthe bacterial and eukaryotic cytoskele-tons.Curr. Opin. Cell Biol.16:24–31. 3. ... Micro-biol. 24:905–15. 32. Erickson HP. 1995. FtsZ, a prokaryotichomolog of tubulin?Cell 80:367–70.

19 Nov 2004: Biol. Chem. 276, 17307–17315 (2001). 24. Andreu, J.M., Oliva, M.A. & Monasterio, O. ... J. Appl. Crystallogr. 24, 946–950 (1991). 41. Miroux, B. & Walker, J.E.

15 Apr 2004: 22|i-j|24) 78 Weak (0–5.0 Å) 183Long range (|i – j| > 4) 163Total 554 (=7.1 per residue)Statistics for 45 accepted structures:. ... J ApplCrystallogr 24: 946–950. Kuroda, A., Asami, Y., and Sekiguchi, J.

2 Jun 2004: glass coverslips in 24-well plates were infected with trans-fected parasites and were processed for IF 20 –24 h post-transfection asdescribed (32). ... 23 Golgi Type I TM 90BAPVSVG 24 Golgi Type I TM 905-poly(L)ext 26 Golgi ND ND.

24 Sep 2004: Received for publication, January 5, 2004, and in revised form, March 22, 2004Published, JBC Papers in Press, March 24, 2004, DOI 10.1074/jbc.M400023200. ... 2002) Biochim. Biophys. Acta 1579, 219 –224. 24. Bishop, N., Horman, A., and Woodman, P.

4 May 2004: Number of Receptors Phase Residual" Weight in Final. scFv35 Film number 4280 3560 23.5 0.18 Film number 4282 4360 24.2 0.23 Film number 4295 4820 16.1 ... Biochemistry 24, 1212- 1221.

15 Jan 2004: PrP 22-24 was detected at 112 days and130 days using the cold PK digestion protocol (Fig. ... 5A, B,and D). Mutant HuPrP 22-24 was found in Tg69 mice at 200days of age (Fig.

29 Dec 2004: Vol. 265, No. 24, Issue of August 25, pp. 14364-14370,199O Printed in V. ... K eq was determined at 0 “C! while Kd was obtained at 24 “C.

20 Sep 2004: Molecular Cell, Vol. 15, 951–964, September 24, 2004, Copyright 2004 by Cell Press.

8 Nov 2004: After 18–24 h, cells were harvested for parallelluciferase measurements (using a Luciferase Reporter Kit fromRoche) and immunoblotting. ... Biochemical analysesExperiments analyzing PrP constructs were performed between 18and 24 h after transfection

24 Sep 2004: Protein atoms 4404 4164 4138Waters 265 324 259Rcrystd 20.2 19.9 22.6Rfreed (% data used) 24.1 (5) 24.3 (5) 26.7 (5)r.m.s.d. ... inosi-in 1 l sitting drops over reservoirs of 24-well plates (Hampton).

21 Dec 2004: Between 5000 and 10,000 cells were plated per well of a 96-well platebetween 24 and 36 h before transfection, at which time they were 40 – 80%confluent. ... Unless other-wise noted in the figure legend, the cells were analyzed 18 –24 h after

1 Nov 2004: Multiplicity (last shell) 14.1 (14.1) 14.3 (14.2) 11.6 (11.6) 7.2 (7.2) 6.9 (3.9) 24.9 (24.0)I/ (last shell)

23 Nov 2004: Transferrin uptake and immunofluorescenceCos 7 cells were transfected using GeneJuice according to manufacturers instructions,plated onto polylysine coated coverslips 24 hours post transfection and assayed fortransferrin uptake 48 hours post

30 Jan 2004: recruit clathrin to membranes. Mammalian am-phiphysin2 has several clathrin-binding sites (24–26), and we found that the wild-type, but notmut1, efficiently recruited clathrin to liposomes(fig. ... It is interesting to note that thestructure of the

23 Nov 2004: Curr. Opin.Cell Biol. 14, 454–462. 24. Seaman, M.N.J., Marcusson, E.G., Cereghino, J.L., and Emr,S.D.

2 Feb 2004: Membrane transport between compartments in eukary-otic cells requires proteins that allow the budding andSCISSION of nascent cargo vesicles from one compartmentand their targeting and fusion with another. Just asSNARES are proposed to be essential

3 Dec 2004: The COOH-termi-nal domains of these proteins contain clath-rin-binding motifs (3, 24 ), as well as Asp-Pro-Phe (DPF)-like a- and b-adaptin–. ... De Camilli, A. T.Brunger, J. Cell Biol. 149, 537 (2000). 24.

29 Dec 2004: rg/ ml for 24 h at 37 “C).

6 Dec 2004: Received for publication, July 20, 2004, and in revised form, August 24, 2004Published, JBC Papers in Press, August 25, 2004, DOI 10.1074/jbc.M408179200. ... MIN6 Cell Culture—MIN6 -cells (24) were used between passages19 and 30 and grown in

23 Nov 2004: Evolving nature of the AP2 a-appendage hubduring clathrin-coated vesicle endocytosis. Gerrit JK Praefcke1,2, Marijn GJ Ford1,Eva M Schmid, Lene E Olesen,Jennifer L Gallop, Sew-Yeu Peak-Chew,Yvonne Vallis, M Madan Babu,Ian G Mills3 and Harvey T

15 Apr 2004: Sec61 Sec61 21.4 3.6 24.8 6.1a 23.9 2.5a. FRET efficiencies (mean SD; n 40) for the indicated donor–acceptorantibody pairs were measured on cells that