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22 Nov 2002: EpsinR: an AP1/clathrin interacting protein. involved in vesicle trafficking. Myc-tagged and endogenous epsinR versus epsinR-GFP. endogenous EpsinR - green (Ra43). AP1 - red. endogenous EpsinR - blue (Ra43). EpsinR-GFP - green,. AP1 - red. EpsinR

20 Mar 2002: B12. 24. 15% PEG 6K, 0.15M KSCN, 0.1M Na Acetate at pH 5.5. ... F12. 24. 15% PEG 6K, 0.15M KSCN, 0.1M TRIS at pH / (HOAc) 7.5.

20 Mar 2002: B12. 24. 0.8M NaFormate, 8% PEG 20K+ 8% PEG550 MME, 0.1M NaCacodylate / (HOAc)at pH 6.5. ... H12. 24. 15% PEG 6K, 0.15M KSCN, 0.1M Na Acetate at pH 4.5.

20 Mar 2002: B12. 24. 20% iso-Propanol, 0.1 M Na Acetate pH 4.6, 0.2 M Calcium Chloride. ... F12. 24. 30% Jeffamine M-600, 0.1 M MES pH 6.5, 0.05 M Cesium Chloride.

3 May 2002: In each of thesecases, purified soluble factors and syntheticliposomes are sufficient to reconstitute coatformation [21–24]. ... Proc. Natl. Acad. Sci. U. S. A. 95,11199–11204. 24 Matsuoka, K.

20 Apr 2002: An initial model wasbuilt using the program O (24). Noncrystallographic symmetryrestrained positional and temperature factor refinement werecarried out with the program X-PLOR. ... S. (1993) Acta Crystallogr. D 49,. 129 –147.24. Jones, T. A., Zou, J.-Y.

14 Jan 2002: 24.Chook, Y.M., Gray, J.V., Ke, H. & Lipscomp, W.N. The monofunctional chorismate mutase from Bacillus subtilis: structure determination of chorismate mutase and its complexes with a transition state ... J. Appl. Cryst. 24, 946-950 (1991). 39.Merrit, E.A.

5 Jul 2002: Cell 1986;47:711– 719. 24. Liao S, Lin J, Do H, Johnson AE. ... Molec Biol Cell 1998;9:2681– 2697. Problems and paradigms. BioEssays 24.8 747.

20 Apr 2002: THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 273, No. 30, Issue of July 24, pp.

20 Apr 2002: Crystal Structure of a Calcium-Phospholipid Binding Domainfrom Cytosolic Phospholipase A2. (Received for publication, September 24, 1997). ... 1991) J. Appl. Crystallogr. 24, 946 –95072. Nicholls, A. (1992) GRASP: Graphical Representation and Analysis

30 Dec 2002: ParM subunits are assem-bled head to tail into a protolament. Each successive subunit is rotatedby 166 and translated by 24.5 AÊ to create a helical lament. ... J. Appl. Crystallogr., 24,946950. Laskowski,R.A., MacArthur,M.W., Moss,D.S. and Thornton,J.M.

30 Jul 2002: age but lost its platform subdomain. The high degree of observation that there is a pool of epsin located at theGolgi [24]. ... 1996). Methods used in271, 12111–12116.the structure determination of bovine mitochondrial F1 ATPase.24.

20 Apr 2002: diC7PC (8 mM) no significant activation observed 0.85( 0.06 24.7( 2.9(1.7( 0.2). ... 132) PI-PLCδ1 and at 24-45 C for full-length PI-PLCδ1 (Figure 7A,B).

18 Apr 2002: B12. 24. 0.2 M Lithium Acetate, 20% PEG 3350. C1. 25. ... F12. 24. 0.2 M Ammonium Acetate, 0.01 M Ca Chloride, 0.05 M Na Cacodylate pH 6.5, 10% PEG 4000.

20 Apr 2002: PLC Activity in Mixed Micelles—The assay of hydrolysis of PIP2 andPI was based on methods described previously (19, 21, 24, 25). ... Thecalcium dependence of PIP2, PIP, and PI hydrolysis was analyzed usinga range of calcium buffers as described

20 Apr 2002: Deletion variants of PLC-N1 and PLC-Qlacking this second lobe are inactive, probably dueto destabilising the fold of the enzyme [23,24].Although sequence similarity throughout the EF-. ... Bio-phys. Res. Commun. 211 (1995) 364369. [24] Y. Emori, Y. Homma,

20 Apr 2002: 24). Thetopology of the domain is identical to that of phospholipase Cd1and the recently reported calcium-independent C2 domainfrom protein kinase Cd (23), whereas it is a circular permuta-tion of ... 24. Sutton, R. B., and Sprang, S. R. (1998) Structure

20 Apr 2002: baculovirus expression of Src family kinases were madeby subcloning the cDNAs (24) encoding activated Src (Y527F Src),activated Fyn (Y531F Fyn), and activated Lck (Y525F Lck) into thebaculovirus transfer vector ... Some of the additional sites couldbe

20 Apr 2002: Negatively charged regions are shown in red and positively chargedin blue (ranging from 18 kT/e to 24 kT/e). ... Crystallogr.heavy-atom parameter refinement for multiple isomorphous re- 24, 946–950.placement and multiwavelength anomalous diffraction

12 Oct 2002: Resolution (AÊ ) 2.0 2.0Observations/unique reections 83 922/24 307 84 280/24 356Completeness (last shell) % 93.6 (74.1) 93.7 (74.1)Rmerge. ... The supernatant was collected anddialysed against 50 mM TrisHCl pH 8.0 and 1.5 M urea for 16 h, andthen

20 Apr 2002: 1996)J.Biochem. 120, 671-676. Kraulis, P. J. (1991)J. Appl. Crystallogr. 24, 946-950.Lee, S.

20 Apr 2002: 4.6)〈I/σ(I)〉b 24.1(5.1) 15.7(4.3) 20.6(4.4) 17.2(4.9).

19 Apr 2002: Molecular Cell, Vol. 9, 773–788, April, 2002, Copyright  2002 by Cell Press. Molecular Architecture of SMC Proteinsand the Yeast Cohesin Complex. other eukaryotes so far studied and several have alsobeen implicated in sister chromatid cohesion

20 Apr 2002: Molecular Cell, Vol. 6, 909–919, October, 2000, Copyright 2000 by Cell Press. Structural Determinants of Phosphoinositide3-Kinase Inhibition by Wortmannin, LY294002,Quercetin, Myricetin, and Staurosporine. for proteins such as protein kinase B

24 Jan 2002: Tranfected. strate to a degradative pathway. Precedence exists for cells were analyzed between 24 and 60 hr after transfection.

31 Oct 2002: Molecular Biology of the CellVol. 13, 3775–3786, November 2002. Cotranslational Partitioning of Nascent Prion Proteininto Multiple Populations at the Translocation ChannelSoo Jung Kim and Ramanujan S. Hegde†. Laboratory of Cellular Oncology,

20 Mar 2002: F12. 24. 0.8M NaFormate, 8% PEG 20K+ 8% PEG550 MME, 0.1M NaAcetate at pH 4.5. ... H12. 24. 0.8M NaFormate, 8% PEG 20K+ 8% PEG550 MME, 0.1M Na Acetate at pH 5.5.

20 Mar 2002: B12. 24. 0.1 M Mg chloride hexahydrate, 0.1 M HEPES-Na / HCl 7.5, 30 %v/v PEG 400. ... F12. 24. 0.1 M CAPSO/NaOH pH 9.5,0.1M NaCl, 0.1M MgCl2, 30% v/v PEG 400.

18 Apr 2002: B12. 24. 35% (v/v) MPD, 0.1M Tris pH 7.0, 0.2 NaCl (final pH 7.0). ... F12. 24. 30% (w/v) PEG-8000, 0.1M Imidazole pH 8.0, 0.2M NaCl (final pH 7.7).

27 Mar 2002: B12. 24. 40% (v/v) PEG-400, 0.1M Na/K phosphate pH 6.2, 0.2 M NaCl (final pH 6.9). ... F12. 24. 25% (v/v) 1,2-propanediol, 10% (v/v) glycerol, 0.1M Tris pH 8.5, 0.2 M MgCl2 (final pH 8.5).

20 Mar 2002: F12. 24. 1.0 M di-Ammonium Phosphate, 0.1 M ADA pH 6.5.

20 Mar 2002: B12. 24. 0.4 M Magnesium Formate, 0.1 M Bis-Tris Propane 7.0.

20 Mar 2002: B11. 23. 2.1 M DL-Malic Acid pH 7.0. B12. 24. 2.8 M Sodium Acetate pH 7.0.

3 May 2002: In each of thesecases, purified soluble factors and syntheticliposomes are sufficient to reconstitute coatformation [21–24]. ... Proc. Natl. Acad. Sci. U. S. A. 95,11199–11204. 24 Matsuoka, K.

29 Apr 2002: an electron-dense core (24).The use of lipophilic FM dyes to follow vesicle cycling has alsosuggested the existence of fast kiss-and-run exocytosis in syn-aptosomes (25) and in ... timedetermined. For control cells, the data are derived from 12 cells and