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LMB Rising Talent Fellowship Additional Information 1. Is the ...
https://www2.mrc-lmb.cam.ac.uk/wordpress/?wpdmdl=12060822 Feb 2024: LMB Rising Talent Fellowship Additional Information 1. Is the LMB Rising Talent Fellowship only for people from a Black background? Yes, this scheme is designed to attract Black researchers. This Fellowship is open to people who self-identify as -
Sup Fig 1
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/YopJSup.pdf11 Nov 2008: HeLa cells that had been serum-starved for 16 hours were stimulated with either EGF (20 ng/ml), TPA (200 ng/ml) or isoproterenol (10 µM) at 37ºC and the activation ... 60. 65. 70. 75. 200 300 400 500 600 700 800 900 1000 1100 12000. -
“The LMB provides an unsurpassed environment for both new ...
https://www2.mrc-lmb.cam.ac.uk/?wpdmdl=1885218 Jan 2021: 50 Group Leaders. 400 postdoctoral/support scientists. 100 PhD students. 200 support staff. -
LIGANDS Meetings ● Previously:– Developer → Users ● Now ...
https://www2.mrc-lmb.cam.ac.uk/coot/ligands/2016/emsley-ccp4-ligands-dec-2016.pdf9 Dec 2016: Why? Hint, hint! Increased to 200 Conformers. Problem 1: (Back to Acedrg dictionary):Use Fixed Atoms. -
Protocol for dragonfly copy
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Manuals/Protocol_mosquito_additive.pdf13 Dec 2017: of tips Duration. 1 MRC plate 100 100 0 2 µL 2 16 104 2 min1 MRC plate 200 200 0 5 µL 3.8 31 104 2min 10 sec2 Costar ... plate 200 200 100 5 µL 3.8 31 200 3min 45 sec2 Costar plate 500 500 100 5 µL 7.4 60 200 4min 15 sec. -
Angstrom Additive Screen Scoring Sheet MD1-100.xlsx Angstrom Additive …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/Addit_02.pdf26 Nov 2015: E12 Tetraethylene glycol, 80% v/v. F1 PEG 200, 20% v/v. F2 PEG 200, 40% v/v. ... F3 PEG 200, 80% v/v. F4 Pentaethylene glycol, 20% v/v. F5 Pentaethylene glycol, 40% v/v. -
Pi-PEG Screen 1.5 disclaimer
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/LMB19b-Pi-PEG_Screen_1.5_disclaimer.pdf27 Sep 2022: Pi-PEG Screen 1.5x Precipitant Buffer Precipitant 2Condition Chemical Concentration Units Chemical Concentration Units pH Chemical Concentration Units 1-1 PEG 200 33.75 % Sodium acetate trihydrate 75 mM ... PEG 1500 22.5 % Sodium acetate trihydrate 75 mM -
doi:10.1016/j.jbbm.2005.12.008
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/rfcloning2006%20published.pdf19 Apr 2006: Miroux personal communication), 200 AM dNTP’s (Roche), 1!reaction buffer,2.5 units PfuTurbok (Stratagene) in a total volume of 50 Al. ... TCF1 200 3–6 2.0 4.6 4/4 OK Yes nd. TCF2 200 3–6 2.0 4.6 4/4 OK Yes nd. -
Protocol for dragonfly
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Manuals/protocols_for_dragonfly.pdf13 Dec 2017: secMAXI 24 200 1.8 2 min 25 secMAXI 48 200 3 4 min 20 sec. -
DOI: 10.1126/science.1136985 , 120 (2007); 316Science et al.Maria C.…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Alonso_Science.pdf6 Apr 2007: on. Apr. il 6,. 200. 7 w. ww. sci. ence. mag. ... REPORTS. on. Apr. il 6,. 200. 7 w. ww. sci. ence. -
ESMS of membrane proteins
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/methods/ESMS%20of%20membrane%20proteins.pdf20 Jul 2007: mix - add 200 μl water, mix vigorously - centrifuge 2 min 10.000x g - take top phase off ( 500 μl), protein forms precipitated layer - add 300 μl methanol - centrifuged 1 min -
Optimization of crystallization conditions with additive screening…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/PresentationsOther/additive-screening.pdf9 Jan 2017: 200 nl protein 200 nl condition 100 nl additive). This late approach facilitates the screening at different concentrations of additives (by simply varying the volume of additive screen added) and implies -
TBS may template.qxd
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsin/EM/mcmahon.pdf3 May 2002: J. Cell. Biol.155, 193–200. f Takei, K. et al. (1999) Functional partnership betweenamphiphysin and dynamin in clathrin-mediated endocytosis. ... J. Cell. Biol. 155,193–200. 20 Takei, K. et al. (1999) Functional partnershipbetween amphiphysin and -
Four-stranded mini microtubules formed by Prosthecobacter BtubAB show …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2017-Deng-E5950-8.pdf29 Dec 2017: anti-biotin antibody. F 127. catastrophe time. 0. 0.5. 1.0. time (seconds) 200 800. ... of the reaction was pipetted onto freshly glow-dischargedCu/Rh Quantifoil R2/2 200 mesh holey carbon grids (Quantifoil). -
Proteins containing photosynthetic reaction centre domains modulate…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Nussbaum_Nat_micro_2024.pdf1 Mar 2024: CdpB2. SepF. SepFCdpB1. 200. Abso. rban. ce (a. u.). Abso. rban. ... For 1 transformation, 200 µl of the prepared cell suspension was used. -
� Supplementary Figure 1 Bacterial dynamin-like protein (BDLP) from…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nature%20BDLP%202006%20sup.pdf28 Sep 2006: 50 nm. 100 nm. 200 nm. I. Supplementary Figure 2. The crystal structure of BDLP in GDP-associated and nucleotide-free conformation. -
A-nsmb855.indd
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nsmb%20FtsZ%202004.pdf19 Nov 2004: sZ-N. TmFt. sZ-C. TmFt. sZ-N. C. TmFtsZ TmFtsZ-N. TmFtsZ-C TmFtsZ-NC. Wavelength (nm)200 210 220 230 240 250 260. ... 081% (w/v) malachite green and 2 ml of water were mixed and 200 µl was added to 50-µl reactions contain-ing proteins or inorganic -
Biol. Chem., Vol. 381, pp. 993 – 999, September/October ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/BiolChem%20Helical%20Tubes.pdf13 Sep 2000: Figure 2D) as published earlier (Löwe and Amos, 1999).Tubes assembled in the presence of GMPCPP show re-peats of 200 Å, 100 Å, 67 Å, and 50 Å (Figure 2E, ... In the case of the GMPCPP-inducedtubes, groups producing the 200 Å longitudinal -
The MORPHEUS protein crystallization screen
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/morpheus%202009.pdf12 Nov 2009: TEN 200 is a buffer containing 20 mM Tris, 1 mM ethylenediaminetetraacetic acid (EDTA), 1 mM sodium azide and 200 mM sodium chloride. ... 1998) G04 oCNVA Concanavalin A 7.0 26.5 Sigma, L7647 Dissolved in TEN 200. -
early in DNA damage response and regulateRAP80 localization. Many ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/MartensSytPap07.pdf16 Oct 2009: REPORTS. on. Oct. ober. 18,. 200. 8 w. ww. sci. ence. ... Pdl indicates the polydispersity index. The actual sizeof the liposomes is significantly smaller for the 800 and 200 liposomes suggestingthat the actual curvature preference of the synaptotagmin -
www.sciencemag.org/cgi/content/full/1164346/DC1 Supporting Online…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Salje%20et%20al%20Science%202009%20Suppl.pdf15 Dec 2008: Membranes were stained using 10 µg/mL FM-464 for ten minutes. 200 µL of cells were taken directly from. -
doi:10.1016/j.cub.2004.02.060
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Zimmerberg_McLaughlin2004.pdf17 Mar 2004: J. Cell Biol. 155,193-200. -
pnas201313978 4601..4610
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/pnas2013duman.pdf23 Dec 2013: Again BSA and 200 mMKCl were included throughout the gradient to increase speci-ficity. ... 7.5. 200. 2.5. 225 250(nm)λ. CD. [mde. g]. - liposomes+ liposomes. -
Wild card macros
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/sciencegayathri2012/Gayathri.SM.pdf23 Oct 2012: The image pixel size at this. magnification was 1.64 Å/pixel. In total of 200 CCD images were collected. ... µl were added to the 200 µl reaction mixture before initiating polymerization with 2 mM (final concentration). -
molcel4221mmc1
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MolCell2012MipZ/mipz2012supp.pdf15 Mar 2012: ParB (200 RU) was immobilized on an SPR sensor chip and allowed to react with wild-type MipZ protein (0.5 µM) that had been preincubated with 2 mM ATP and ... Subsequently, the protein was applied to a Sephacryl S-200 gel filtration column (GE Healthcare -
d402_16g 822..826
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/15_Hegde_RS_Nature_1999.pdf9 Dec 1999: sym. pto. mat. ic. Age (days)200 400 600 800. c. b(A. ... 80. 60. 40. 20. 00 100 200 300 400. (A117V) H(STE). -
CAN-11-2008 3642..3651
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/79_Basseville_A_CanRes_2012.pdf24 Sep 2015: Valproic acidinduced the weakest effect on mRNA and protein ABCG2expression (150%–200% increase). ... 100. 150. 200. 250. 300. 350. 400. 450. 500WT Q141K WT Q141K WT Q141K. -
MeganPalm
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/MeganPalm.pdf3 Dec 2005: stops when more than 200 pixels are inside the region. The directional dilation forces. ... 200 eggs from a variety of mutant types and recording conditions illustrate the. -
Regulation of lipid binding underlies the activation mechanism of…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/22120714.pdf21 Nov 2012: ni. WT/. icW. T/i. 0. 100. 200. 300. 400. CD/n. ic. ... 100. 200. 300. ΔReq. (R. U). 0100200300400500600700800. SH2 deletion lipid kinase dead cancer-linkedmutations:. -
Structure of bacterial tubulin BtubA�B:Evidence for horizontal gene…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/btubab%202005.pdf21 Jun 2005: Buffer con-tained 100 mM PipesNaOH and 200 mM KCl (pH 6.8). ... 1B and 2. (Scale bar: 200 nm.). Schlieper et al. PNAS June 28, 2005 vol. -
pone.0107211 1..10
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/leoa2014.pdf3 Sep 2014: 37uC. At mid-exponential growth phase, 200 ml of cell culturewere transferred to 1 ml of cold methanol and kept at 220uC forat least 60 min. ... Invitrogen) at a 1:200 dilution. Cells were observed using a Leica. -
Asgard archaea shed light on the evolutionary origins of the…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Hatano%20Nat%20Comms%202022.pdf13 Jun 2022: 150. 50. 250. mAU. 100. 200. 150. 50. 250. mAU. elution volume (ml)5 1510 20. ... 12.4 mlβ-amylase(200 kDa). 18.9 mlCytochrome c. (12.4 kDa). 17.4 mlCarbonic anhydrase. -
cm6307.qxd
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/TAXOL.C&B.pdf18 Feb 1999: 110 120 130 140 150 160 170 180 190 200. 210 220 230 240 250 260 270 280 290 300. -
Phil. Trans. Roy. Soc. Lond. B. 261, 95-104 (1971) ...
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ptrs71.pdf26 Nov 2012: In some experiments of this sort, using a range 0 to 200 V, the potential was found to vary along the thread proportionately to the electron flux. ... manner. The corresponding interference patterns indicate a n ear-zero potential, a high positive -
PII: S0014-5793(01)02216-5
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MinD%20FEBS.pdf5 Mar 2001: The protein was loaded directly onto a Sephacryl S200column (Amersham-Pharmacia) equilibrated in 200 mM NaCl, 20mM Tris, 1 mM EDTA and 1 mM NaN3 pH 7.0. -
mmi_4133.fm
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Spo0J%202004.pdf30 Jun 2004: Spo0J] (mM)0 25 50 100 200 300 25 50 100 200 300. ... The protein was buffer exchanged into20 mM Tris, pH 8.5, 200 mM NaCl using a Sephadex G25 gelfiltration column (Amersham). -
MinCD cell division proteins form alternating copolymeric cytomotive…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/ncomms6341.pdf6 Dec 2014: 200. 250. MinDATP. ATP/ATPγS. EcMinD (D154A): non-polymerising. D155A. minB – minB – slmA–. ... 200. 250. 300. Inte. nsity M. Figure 3 | MinCD filaments bind to membrane and are disassembled by MinE. -
Acetylation of MEK2 and I{kappa}B kinase (IKK) activation loop…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/YopJ.pdf28 Nov 2006: Acetyl Transferase Assays. GST-MEK2 (0.5 g) (purified by usingstandard protocols for GST-tagged protein purification) wasincubated with varying amounts (0 –200 ng) of (His)6-YopJ-wt ... Stimulation of MEK and Erk phosphorylation by various -
“The LMB provides an unsurpassed environment for both new ...
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/www2.mrc-lmb.cam.ac.uk/?wpdmdl=1885218 Jan 2021: 50 Group Leaders. 400 postdoctoral/support scientists. 100 PhD students. 200 support staff. -
61 Ultramicroscopy 25 (1988) 279-292 North-HoUand, Amsterdam 279…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ultram88.pdf1 Apr 2016: Each image of a tube consisted typically of 1200 X 200 pixels ( 5 000 X 1000 A), and was padded to a 1536 X 512 array size, from which Fourier ... Second-order effects, e.g. mul-tiple scattering, were barely detectable in the nega-tive stain study, where -
318-320 News Feat Curves MH.indd
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/NatureAheadCurve.pdf17 Jul 2009: 3. Farsad K. et al. J. Cell Biol. 155, 193–200 (2001).4. -
53091 361..366
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/McMahonE.pdf3 Dec 2004: interactions. J. Cell Biol. 155, 193–200 (2001). 20. Zhang, B. et al. -
14821680963433 1..18
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/e21600-download.pdf19 Dec 2016: 200 mM NaCl, 1 mM DTT, 10% glycerol (v/v), 10 mM imidazole, pH 8.0) supplemented with DNase. ... Stock et al., 2005). After optimisation, the best crystals were obtained in 200 nl drops composed. -
cde235 2454..2461
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MinC%20EMBO%202001.pdf4 May 2001: 200 column buffer. ... solution, andadditionally 200 mM NaI in the drop. -
Structural insight into the ESCRT-I/-II link and itsrole in ...
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17215868.pdf14 Jun 2007: 0 50 100 150 200. 0.22. 0.27 27 nM. 61 nM. ... Superdex 200 10/300 column. 16 20128. 6 10 14 18Elution volume (ml). -
DOI: 10.1126/science.1135394 , 239 (2007); 317Science et al.Nabil…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17626883.pdf8 Dec 2007: 150. 200. WT E542K E545K Q546K- - - - p85ni. p110 α. ... 0. 50. 100. 150. 200. 250. - - Phosphopeptide. C. B. -
Structure of the hexagonal surface layer on Caulobacter crescentus…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/s-layer-nmicrobiol201759.pdf6 Apr 2017: Crystals wereflash-frozen in liquid nitrogen for data collection using an additional 25% (vol/vol)PEG 200 as cryo-protectant. ... 200 mesh grids in a Vitrobot Mark IV (FEICompany) maintained at 10 C at 100% relative humidity. -
Chapter 20 In Vitro Dissection of Protein Translocationinto the ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/62_Sharma_AS_MMB_2010.pdf30 Apr 2010: 4. 100 mM CaCl2: Dissolve in water. Keep at 4C.5. 200 mM EGTA. ... A good range to start is between 30and 200 µL of packed bead volume. -
Identifying proteins bound to native mitotic ESC chromosomes reveals…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Djeghloul%20Nat%20Camms%202020.pdf11 Aug 2020: itotic. chro. mos. omes. Centrifugation 200 g. Supernatant containingchromosomes. Centrifugation 18,000 g(to pellet chromatin). -
Bacterial chromosome segregation: structure andDNA binding of the Soj …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/soj_embo2005.pdf30 Jan 2005: mol. Pi p. rodu. ced/. mol. Soj. 0 50 100 150 200.
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