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  2. LMB Rising Talent Fellowship Additional Information 1. Is the ...

    https://www2.mrc-lmb.cam.ac.uk/wordpress/?wpdmdl=1206082
    2 Feb 2024: LMB Rising Talent Fellowship Additional Information 1. Is the LMB Rising Talent Fellowship only for people from a Black background? Yes, this scheme is designed to attract Black researchers. This Fellowship is open to people who self-identify as
  3. Sup Fig 1

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/YopJSup.pdf
    11 Nov 2008: HeLa cells that had been serum-starved for 16 hours were stimulated with either EGF (20 ng/ml), TPA (200 ng/ml) or isoproterenol (10 µM) at 37ºC and the activation ... 60. 65. 70. 75. 200 300 400 500 600 700 800 900 1000 1100 12000.
  4. “The LMB provides an unsurpassed environment for both new ...

    https://www2.mrc-lmb.cam.ac.uk/?wpdmdl=18852
    18 Jan 2021: 50 Group Leaders. 400 postdoctoral/support scientists. 100 PhD students. 200 support staff.
  5. LIGANDS Meetings ● Previously:– Developer → Users ● Now ...

    https://www2.mrc-lmb.cam.ac.uk/coot/ligands/2016/emsley-ccp4-ligands-dec-2016.pdf
    9 Dec 2016: Why? Hint, hint! Increased to 200 Conformers. Problem 1: (Back to Acedrg dictionary):Use Fixed Atoms.
  6. Protocol for dragonfly copy

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Manuals/Protocol_mosquito_additive.pdf
    13 Dec 2017: of tips Duration. 1 MRC plate 100 100 0 2 µL 2 16 104 2 min1 MRC plate 200 200 0 5 µL 3.8 31 104 2min 10 sec2 Costar ... plate 200 200 100 5 µL 3.8 31 200 3min 45 sec2 Costar plate 500 500 100 5 µL 7.4 60 200 4min 15 sec.
  7. Angstrom Additive Screen Scoring Sheet MD1-100.xlsx Angstrom Additive …

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/Addit_02.pdf
    26 Nov 2015: E12 Tetraethylene glycol, 80% v/v. F1 PEG 200, 20% v/v. F2 PEG 200, 40% v/v. ... F3 PEG 200, 80% v/v. F4 Pentaethylene glycol, 20% v/v. F5 Pentaethylene glycol, 40% v/v.
  8. Pi-PEG Screen 1.5 disclaimer

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/LMB19b-Pi-PEG_Screen_1.5_disclaimer.pdf
    27 Sep 2022: Pi-PEG Screen 1.5x Precipitant Buffer Precipitant 2Condition Chemical Concentration Units Chemical Concentration Units pH Chemical Concentration Units 1-1 PEG 200 33.75 % Sodium acetate trihydrate 75 mM ... PEG 1500 22.5 % Sodium acetate trihydrate 75 mM
  9. Protocol for dragonfly

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Manuals/protocols_for_dragonfly.pdf
    13 Dec 2017: secMAXI 24 200 1.8 2 min 25 secMAXI 48 200 3 4 min 20 sec.
  10. doi:10.1016/j.jbbm.2005.12.008

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/rfcloning2006%20published.pdf
    19 Apr 2006: Miroux personal communication), 200 AM dNTP’s (Roche), 1!reaction buffer,2.5 units PfuTurbok (Stratagene) in a total volume of 50 Al. ... TCF1 200 3–6 2.0 4.6 4/4 OK Yes nd. TCF2 200 3–6 2.0 4.6 4/4 OK Yes nd.
  11. DOI: 10.1126/science.1136985 , 120 (2007); 316Science et al.Maria C.…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Alonso_Science.pdf
    6 Apr 2007: on. Apr. il 6,. 200. 7 w. ww. sci. ence. mag. ... REPORTS. on. Apr. il 6,. 200. 7 w. ww. sci. ence.
  12. ESMS of membrane proteins

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/methods/ESMS%20of%20membrane%20proteins.pdf
    20 Jul 2007: mix - add 200 μl water, mix vigorously - centrifuge 2 min 10.000x g - take top phase off ( 500 μl), protein forms precipitated layer - add 300 μl methanol - centrifuged 1 min
  13. Optimization of crystallization conditions with additive screening…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/PresentationsOther/additive-screening.pdf
    9 Jan 2017: 200 nl protein 200 nl condition 100 nl additive). This late approach facilitates the screening at different concentrations of additives (by simply varying the volume of additive screen added) and implies
  14. TBS may template.qxd

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsin/EM/mcmahon.pdf
    3 May 2002: J. Cell. Biol.155, 193–200. f Takei, K. et al. (1999) Functional partnership betweenamphiphysin and dynamin in clathrin-mediated endocytosis. ... J. Cell. Biol. 155,193–200. 20 Takei, K. et al. (1999) Functional partnershipbetween amphiphysin and
  15. Four-stranded mini microtubules formed by Prosthecobacter BtubAB show …

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2017-Deng-E5950-8.pdf
    29 Dec 2017: anti-biotin antibody. F 127. catastrophe time. 0. 0.5. 1.0. time (seconds) 200 800. ... of the reaction was pipetted onto freshly glow-dischargedCu/Rh Quantifoil R2/2 200 mesh holey carbon grids (Quantifoil).
  16. Proteins containing photosynthetic reaction centre domains modulate…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Nussbaum_Nat_micro_2024.pdf
    1 Mar 2024: CdpB2. SepF. SepFCdpB1. 200. Abso. rban. ce (a. u.). Abso. rban. ... For 1 transformation, 200 µl of the prepared cell suspension was used.
  17. � Supplementary Figure 1 Bacterial dynamin-like protein (BDLP) from…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nature%20BDLP%202006%20sup.pdf
    28 Sep 2006: 50 nm. 100 nm. 200 nm. I. Supplementary Figure 2. The crystal structure of BDLP in GDP-associated and nucleotide-free conformation.
  18. A-nsmb855.indd

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nsmb%20FtsZ%202004.pdf
    19 Nov 2004: sZ-N. TmFt. sZ-C. TmFt. sZ-N. C. TmFtsZ TmFtsZ-N. TmFtsZ-C TmFtsZ-NC. Wavelength (nm)200 210 220 230 240 250 260. ... 081% (w/v) malachite green and 2 ml of water were mixed and 200 µl was added to 50-µl reactions contain-ing proteins or inorganic
  19. Biol. Chem., Vol. 381, pp. 993 – 999, September/October ...

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/BiolChem%20Helical%20Tubes.pdf
    13 Sep 2000: Figure 2D) as published earlier (Löwe and Amos, 1999).Tubes assembled in the presence of GMPCPP show re-peats of 200 Å, 100 Å, 67 Å, and 50 Å (Figure 2E, ... In the case of the GMPCPP-inducedtubes, groups producing the 200 Å longitudinal
  20. The MORPHEUS protein crystallization screen

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/morpheus%202009.pdf
    12 Nov 2009: TEN 200 is a buffer containing 20 mM Tris, 1 mM ethylenediaminetetraacetic acid (EDTA), 1 mM sodium azide and 200 mM sodium chloride. ... 1998) G04 oCNVA Concanavalin A 7.0 26.5 Sigma, L7647 Dissolved in TEN 200.
  21. www.sciencemag.org/cgi/content/full/1164346/DC1 Supporting Online…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Salje%20et%20al%20Science%202009%20Suppl.pdf
    15 Dec 2008: Membranes were stained using 10 µg/mL FM-464 for ten minutes. 200 µL of cells were taken directly from.

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