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11 May 2007: Consider that a homogeneouslydistributed fluorescent protein must be presentat 200 nM to be visualized over backgroundcellular fluorescence (Niswender et al., 1995).Thus, experimental questions involving cellu-lar expression of a fluorescently

25 Sep 2013: input IP. GFPAni1-409GFP. SyndFLAG. Ani1-409GFP. GFP. 200. valu. e. 100. 200. ... Scale bars represent 1 mm and 200 nm for low magnification and high magnificationimages, respectively.

16 Jun 2006: 5) , andthe i r w i dth rema i ned fa i r l y constant (800 - 1 , 200 A).

14 Nov 2014: loaded onto a 16/600 Superdex 200 gel filtration column (GE Healthcare) in 50 mM Tris pH 7.5, 150 mM NaCl, 1 mM βME. ... Crystals were cryo-protected by adding reservoir solution supplemented with 50-60 % (w/v) PEG 200, before flash freezing in liquid

19 Dec 2016: 200 mM NaCl, 1 mM DTT, 10% glycerol (v/v), 10 mM imidazole, pH 8.0) supplemented with DNase. ... Stock et al., 2005). After optimisation, the best crystals were obtained in 200 nl drops composed.

15 Mar 2018: As the curvature of MreB filaments bound to liposomes is much greater (200 nm diameter.

29 May 2019: A) Cells expressing GFP-PrP and GFP-PrP were incubated on. ice with 200 nM extracellular Alexa647-conjugated Nb and Alexa647 fluorescence was measured by flow cytometry. ... pro. port. ion o. f cells. time (min). 0 50 100 150 200.

12 Jun 2020: 6Y4L). The heterodimer consists of EMC2 (residues 11–274), depicted in blue, and EMC9 (residues 1–200),. ... of atoms 9412. Protein residues 1906. Ramachandran Outliers (%) 0.49. Mean B-factors (2) 200.0.

24 Jul 2020: For correspondence:. rhegde@mrc-lmb.cam.ac.uk. †These authors contributed. equally to this work. Competing interest: See. page 25. Funding: See page 25. Received: 15 June 2020. Accepted: 10 July 2020. Published: 13 July 2020. Reviewing editor:

15 Sep 2020: For correspondence:. jyl@mrc-lmb.cam.ac.uk (JL);. ashley.nasmyth@bioch.ox.ac.uk. (KAN). †These authors contributed. equally to this work. Competing interests: The. authors declare that no. competing interests exist. Funding: See page 33. Received: