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31 Jan 2020: Superose 6 Increase 3.2/300 column (GE Healthcare), equilibrated with buffer T200 (50 mM Tris-HCl, 200 mM NaCl, pH 8.0). ... equili-brated with 20 mM Hepes, 200 mM NaCl at pH 7.5.

1 Jul 2011: Von großem Vorteil ist dabei diedynamische Instabilität der ParM-Filamente:Die sich schnell wiederholende Abfolge vonFilamentbildung und -zerfall (ungefähr 200-mal schneller als bei Aktin) ermöglicht esParM, nach den Plasmiden zu

21 Dec 2004: Unless specifically noted otherwise, transfections with Lipo-fectamine 2000 (Invitrogen, Carlsbad, CA) were performed in 96-well plateswith a total of 200 ng of plasmid DNA per well. ... Of this 200 ng, the luciferasereporter plasmid was always kept

17 Mar 2003: O.B., Jr., Krupnick, J.G., Vsevolod, V., Gurevich, V.V.,solution (30% PEG MME 5000, 200 mM (NH4)2SO4, 100 mM MESBenovic, J.L., and Keen, J.H. ... Hao, W., Tan, Z., Prasad, K., Reddy, K.K., Chen, J., Prestwich, G.D.,vent consisting of 40% PEG 400, 200 mM

9 Jan 2017: 200 nl protein 200 nl condition 100 nl additive). This late approach facilitates the screening at different concentrations of additives (by simply varying the volume of additive screen added) and implies

2 May 2013: mAU. 10 μM 5 μM. 1.5 μM. 5 10 15 200.

26 Feb 2019: reconstituted in buffer containing 40 mM NaCl, 2 mM MgCl2 (red) and doublet MukBEF (MukBEFD) reconstituted in buffer containing 200 mM NaCl (green).

29 Dec 2017: anti-biotin antibody. F 127. catastrophe time. 0. 0.5. 1.0. time (seconds) 200 800. ... of the reaction was pipetted onto freshly glow-dischargedCu/Rh Quantifoil R2/2 200 mesh holey carbon grids (Quantifoil).

24 Jan 2009: Molecular Biology of the CellVol. 19, 5093–5103, December 2008. Synaptotagmin-1 Utilizes Membrane Bending and SNAREBinding to Drive Fusion Pore ExpansionKara L. Lynch, Roy R.L. Gerona, Dana M. Kielar, Sascha Martens,†Harvey T. McMahon,† and

30 Jun 2004: Spo0J] (mM)0 25 50 100 200 300 25 50 100 200 300. ... The protein was buffer exchanged into20 mM Tris, pH 8.5, 200 mM NaCl using a Sephadex G25 gelfiltration column (Amersham).