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ITC_sample_preparation_310713
https://facilities.bioc.cam.ac.uk/files/media/itc_sample_preparation_310713.pdf31 Jan 2021: µL syringe 200 µL for filling); For iTC200 (7 less volume; 2.5 more sensitive), 300 µL of protein for sample cell (202 µL cell 80 µL dead volume) 60 µL -
Standard_operating_procedure_for_Aviv_410_CD_instrument
https://facilities.bioc.cam.ac.uk/files/media/standard_operating_procedure_for_aviv_410_cd_instrument.pdf31 Jan 2021: Make a note of the N2 level in the bottle (a full bottle reads 200 Litre). ... The current total cost for a 200 Litre pure nitrogen bottle is £42.00. -
ITC_sample_preparation_310713
https://facilities.bioc.cam.ac.uk/sites/facilities.bioc.cam.ac.uk/files/media/itc_sample_preparation_310713.pdf31 Jan 2021: µL syringe 200 µL for filling); For iTC200 (7 less volume; 2.5 more sensitive), 300 µL of protein for sample cell (202 µL cell 80 µL dead volume) 60 µL -
Standard_operating_procedure_for_Aviv_410_CD_instrument
https://facilities.bioc.cam.ac.uk/sites/facilities.bioc.cam.ac.uk/files/media/standard_operating_procedure_for_aviv_410_cd_instrument.pdf31 Jan 2021: Make a note of the N2 level in the bottle (a full bottle reads 200 Litre). ... The current total cost for a 200 Litre pure nitrogen bottle is £42.00. -
Table Of Contents
https://facilities.bioc.cam.ac.uk/files/media/itc200_user_manual_rev_d.pdf31 Jan 2021: This requires approximately 250 μL per cell even though the working volume of the cell is only 200 μL. ... volume = 20 μl, duration = 200 seconds, spacing = 300 seconds). -
Table Of Contents
https://facilities.bioc.cam.ac.uk/sites/facilities.bioc.cam.ac.uk/files/media/itc200_user_manual_rev_d.pdf31 Jan 2021: This requires approximately 250 μL per cell even though the working volume of the cell is only 200 μL. ... volume = 20 μl, duration = 200 seconds, spacing = 300 seconds). -
NMR_sample_preparation
https://facilities.bioc.cam.ac.uk/files/media/nmr_sample_preparation_210311.pdf1 Feb 2021: best to use deuterated versions of these compounds (please discuss this with the facility staff in advance) - salt concentration (total ionic strength) 200 mM for room temperature probes, 100 mM for -
NMR_sample_preparation
https://facilities.bioc.cam.ac.uk/sites/facilities.bioc.cam.ac.uk/files/media/nmr_sample_preparation_210311.pdf1 Feb 2021: best to use deuterated versions of these compounds (please discuss this with the facility staff in advance) - salt concentration (total ionic strength) 200 mM for room temperature probes, 100 mM for -
Biosensor Selection Guide | Fortebio
https://facilities.bioc.cam.ac.uk/files/media/biosensor-selection-guide.pdf17 Mar 2021: Not testedProtein dependent,. typically 0.25–200 µg/mL. Protein dependent, typically. 0.25–200 µg/mL. ... Protein and protocol (time and rpm). dependent, 0.1–200 µg/mL. Protein dependent, typically. -
Biosensor Selection Guide | Fortebio
https://facilities.bioc.cam.ac.uk/sites/facilities.bioc.cam.ac.uk/files/media/biosensor-selection-guide.pdf17 Mar 2021: Not testedProtein dependent,. typically 0.25–200 µg/mL. Protein dependent, typically. 0.25–200 µg/mL. ... Protein and protocol (time and rpm). dependent, 0.1–200 µg/mL. Protein dependent, typically.
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