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A Polymerization-Associated Structural Switch in FtsZ That Enables…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mBio-2017-Wagstaff-.pdf3 May 2017: All images are at the same magnification (scale bar, 200 nm). ... However, these filaments are not the sameas the open-form filaments, with a much smaller interface buried surface area (BSA) of700 Å2, compared to 1,200 Å2 for 1FOf and PDB -
Four-stranded mini microtubules formed by Prosthecobacter BtubAB show …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2017-Deng-E5950-8.pdf29 Dec 2017: anti-biotin antibody. F 127. catastrophe time. 0. 0.5. 1.0. time (seconds) 200 800. ... of the reaction was pipetted onto freshly glow-dischargedCu/Rh Quantifoil R2/2 200 mesh holey carbon grids (Quantifoil). -
Robotic crystallisation: Consumables
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/consumables.html5 Jun 2017: Swissci. MMX01-UVP. The MRC MAXI 1-drop plate for scale-up/optimization. Reservoir recommended volume: 200 µL. -
Uncovering the action of a selective holophosphatase inhibitor - MRC…
https://www2.mrc-lmb.cam.ac.uk/uncovering-action-selective-holophosphatase-inhibitor/31 Jul 2017: There are over 200 different regulatory subunits that can combine with PP1 to form holophosphatases which dephosphorylate a wide range of proteins in cells. ... There are more than 200 such phosphatases in humans that could in principle be studied using -
IL-17 is a neuromodulator of Caenorhabditis elegans sensory responses
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Nature_2017.pdf13 Jan 2017: 0 100 200 3001. 1.5. 2. 2.5. Time (s). YFP. /CFP. ... 2.5. YFP. /CFP. rat. io. 0 100 200 300 4001. 1.5. -
doi:10.1016/j.pbiomolbio.2004.07.009
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Publications/Robotic_nanolitre_protein_crystallisation.pdf22 Sep 2017: available: the IMPAX and ORYX systems from Douglas Instruments Ltd., UK, and theCyberlab 200, distributed by Gilson Inc., USA. -
REPORT◥ PROTEOSTASIS UBE2O is a quality control factor fororphans ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017b.pdf4 Aug 2017: 1, A and B). Cross-linking reactionsof the active fractions revealed interacting part-ners of 200, 120, and 100 kD (Fig. ... The gradients were either 2 ml or 0.2 ml, on which either 200 µl or 20 µl of sample were layered. -
Optimization of crystallization conditions with additive screening…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/PresentationsOther/additive-screening.pdf9 Jan 2017: 200 nl protein 200 nl condition 100 nl additive). This late approach facilitates the screening at different concentrations of additives (by simply varying the volume of additive screen added) and implies -
Structure of the hexagonal surface layer on Caulobacter crescentus…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/s-layer-nmicrobiol201759.pdf6 Apr 2017: Crystals wereflash-frozen in liquid nitrogen for data collection using an additional 25% (vol/vol)PEG 200 as cryo-protectant. ... 200 mesh grids in a Vitrobot Mark IV (FEICompany) maintained at 10 C at 100% relative humidity. -
REPORT◥ PROTEIN HOMEOSTASIS Mechanistic basis for a moleculartriage…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017.pdf19 Jan 2017: vector and a 3’ primer that anneals 200 bp downstream of the stop codon of the open reading frame. ... DTT). During the buffer change, 1:200 of SuperTEV (for His-tagged proteins) or 3C protease (for GST-tagged proteins) were added for overnight
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