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  2. Four-stranded mini microtubules formed by Prosthecobacter BtubAB show …

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2017-Deng-E5950-8.pdf
    29 Dec 2017: anti-biotin antibody. F 127. catastrophe time. 0. 0.5. 1.0. time (seconds) 200 800. ... of the reaction was pipetted onto freshly glow-dischargedCu/Rh Quantifoil R2/2 200 mesh holey carbon grids (Quantifoil).
  3. Protocol for dragonfly copy

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Manuals/Protocol_mosquito_additive.pdf
    13 Dec 2017: of tips Duration. 1 MRC plate 100 100 0 2 µL 2 16 104 2 min1 MRC plate 200 200 0 5 µL 3.8 31 104 2min 10 sec2 Costar ... plate 200 200 100 5 µL 3.8 31 200 3min 45 sec2 Costar plate 500 500 100 5 µL 7.4 60 200 4min 15 sec.
  4. Protocol for dragonfly

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Manuals/protocols_for_dragonfly.pdf
    13 Dec 2017: secMAXI 24 200 1.8 2 min 25 secMAXI 48 200 3 4 min 20 sec.
  5. LMB cycles to first place in September Challenge - MRC Laboratory of…

    https://www2.mrc-lmb.cam.ac.uk/lmb-cycles-first-place-september-challenge/
    Thumbnail for LMB cycles to first place in September Challenge - MRC Laboratory of Molecular Biology 1 Nov 2017: This year’s Cycle September challenge was the main #YearoftheBike event organised by Love to Ride as part of the bicycle’s 200.
  6. doi:10.1016/j.pbiomolbio.2004.07.009

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Publications/Robotic_nanolitre_protein_crystallisation.pdf
    22 Sep 2017: available: the IMPAX and ORYX systems from Douglas Instruments Ltd., UK, and theCyberlab 200, distributed by Gilson Inc., USA.
  7. REPORT◥ PROTEOSTASIS UBE2O is a quality control factor fororphans ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017b.pdf
    4 Aug 2017: 1, A and B). Cross-linking reactionsof the active fractions revealed interacting part-ners of 200, 120, and 100 kD (Fig. ... The gradients were either 2 ml or 0.2 ml, on which either 200 µl or 20 µl of sample were layered.
  8. Uncovering the action of a selective holophosphatase inhibitor - MRC…

    https://www2.mrc-lmb.cam.ac.uk/uncovering-action-selective-holophosphatase-inhibitor/
    Thumbnail for Uncovering the action of a selective holophosphatase inhibitor - MRC Laboratory of Molecular Biology 31 Jul 2017: There are over 200 different regulatory subunits that can combine with PP1 to form holophosphatases which dephosphorylate a wide range of proteins in cells. ... There are more than 200 such phosphatases in humans that could in principle be studied using
  9. Robotic crystallisation facility: overview

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/robot-overview.html
    5 Jun 2017: Drops are set with nanolitre dispensing robots (mainly TTP's MOSQUITO). A common approach is single drops of 200 nl final volume with 1:1 ratio (100 nl protein 100 nl ... Larger volume MAXI plates and the corresponding protocols are available for
  10. Robotic crystallisation: Consumables

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/consumables.html
    5 Jun 2017: Swissci. MMX01-UVP. The MRC MAXI 1-drop plate for scale-up/optimization. Reservoir recommended volume: 200  µL.
  11. A Polymerization-Associated Structural Switch in FtsZ That Enables…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mBio-2017-Wagstaff-.pdf
    3 May 2017: All images are at the same magnification (scale bar, 200 nm). ... However, these filaments are not the sameas the open-form filaments, with a much smaller interface buried surface area (BSA) of700 Å2, compared to 1,200 Å2 for 1FOf and PDB

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