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Wolbachia‐mediated protection against viruses in the…
www.jiggins.gen.cam.ac.uk/pdfs/cattel2016b.pdf5 Dec 2016: tions were performed in 25 ll volumes containing 100 lM. Desoxyribonucleotides (dNTP), 200 nM primers, 0.5 Interna-. ... The 10 ll reaction. mix contained 200 nM of each primer, 5 ll Sso Advanced Uni-. -
Wolbachia in European Populations of the Invasive Pest Drosophila…
www.jiggins.gen.cam.ac.uk/pdfs/cattel2016.pdf22 Jul 2016: PCR reactions were performedin 25 μL volumes containing 100 μM dNTP, 200 nM primers, 20mM Dream Taq Green Buffer,0.5 IU Taq DNA polymerase (Eurobio) and 1μL of DNA template. ... The 10μL reaction mixture contained 200 nM of each primer, 5 μL ofLight -
Wolbachia Blocks Viral Genome Replication Early in Infection without…
www.jiggins.gen.cam.ac.uk/pdfs/rainey2016.pdf26 Apr 2016: In the Wolba-chia infected cells we observed a 200–600 fold decrease in FFLuc readout, a marker expressedfrom the RNA produced from subgenomic promoter, which is significantly greater (T-TestP -
REV_ISS_WEB_MEC_13769_25-20 5228..5241
www.jiggins.gen.cam.ac.uk/pdfs/cogni2016.pdf10 Oct 2016: The genetic architecture of resistance to virus infectionin Drosophila. RODRIGO COGNI,†1 CHUAN CAO,1 JONATHAN P. DAY, CALUM BRIDSON andFRANCIS M. JIGGINS. Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK, †Department of -
piRNA pathway is not required for antiviral defense in Drosophila…
www.jiggins.gen.cam.ac.uk/pdfs/petit2016.pdf22 Jul 2016: 0. 200. 400. 2.103. read. cou. nts. 5 10 20 25 3015distance. ... 40. 40. genomes coordinates (x103). viral small RNA. 0 50 100 150 200. -
on July 22, 2016http://rspb.royalsocietypublishing.org/Downloaded…
www.jiggins.gen.cam.ac.uk/pdfs/martinez2016.pdf22 Jul 2016: Populations were kept at 188C in cages (90 mmdiameter 200 mm height) containing a 90 mm Petri dish of stan-dard cornmeal food replaced every 3 days. -
on January 27, 2016http://rspb.royalsocietypublishing.org/Downloaded…
www.jiggins.gen.cam.ac.uk/pdfs/palmer2016.pdf27 Jan 2016: 1 300. 200. 100. 0. –2. –3. –4. –5. –6. log 2. -
Genome Sequencing of the Phytoseiid Predatory Mite Metaseiulus…
www.jiggins.gen.cam.ac.uk/pdfs/hoy2016.pdf22 Jul 2016: 200.7 kb and a scaffold N50 of almost 900 kb (table 1). ... gN. 50. (bp. )200,7. 06. 2,2. 62. 2,9. 42. 8,5. 38.
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