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  2. A positively charged channel within the Smc1/Smc3 hinge required for…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embosmcjingekurze2010.pdf
    1 Dec 2010: Smc1DDSmc3AAA. ARS207 ARS231. 150 160 170 180 190 200. 10.0. 5.01.00.0. ... 5.0. 1.00.0. Smc1DD E1158QSmc3AAA. ARS207 ARS231. 150 160 170 180 190 200.
  3. David Komander to receive Young Investigator Programme EMBO Award -…

    https://www2.mrc-lmb.cam.ac.uk/david-komander-to-receive-young-investigator-programme-embo-award/
    Thumbnail for David Komander to receive Young Investigator Programme EMBO Award - MRC Laboratory of Molecular Biology 29 Nov 2010: David will join a vibrant network of 200 Young Investigators from all over Europe.
  4. 201010176 1..6

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2010-Aylett-TubZ.pdf
    21 Oct 2010: All GTPγS-boundsubunits also retain a Mg2þ ion although only four of 11 GDP-bound subunits do so, despite crystallization with 200 mM MgCl2.When aspartate 64 in H2 is not
  5. The Acetyltransferase Activity of the Bacterial Toxin YopJ ofYersinia …

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Mittal_JBC2010.pdf
    4 Oct 2010: Bound material was elutedfrom the resin using 10 ml of 2 N hydrochloric acid, lyophilizedto remove the acid, and taken up in 200 l water. ... time in a 200-l volume using a FluoroMax-2spectrofluorometer (Horiba Yvon Spex).
  6. Many bacterial plasmids are maintained at low copy number ...

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nrmicro2425.pdf
    14 Sep 2010: Many bacterial plasmids are maintained at low copy number (1–5 copies per chromosome equivalent). To prevent plasmid loss at cell division, plasmids have evolved active segregation mechanisms that are loosely analogous to the spindle apparatus in
  7. MRC plate poster 18 8 2010 hr

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/PresentationsOther/Poster%20Plates.pdf
    6 Sep 2010:  reservoir holds 200 µl.  use robot or manually dispense 0.1 - 10 µl of reservoir and protein.
  8. ©20 10 N atur e A mer ica, Inc. ...

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/20081827.pdf
    2 Aug 2010: After a wash step with buffer A, the column was eluted using a gradient from 0–100% buffer B (buffer A 200 mM imidazole).
  9. A ribosome-associating factor chaperones tail-anchored membrane…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/66_Mariappan_M_Nature_2010.pdf
    1 Aug 2010: The translation timefor RNC preparation was 15 min at 32 uC, after which the samples (volumetypically 200 ml) were chilled on ice and immediately layered on 2-ml 10–50%. ... Centrifugation was for 1 h at55,000 r.p.m. at 4 uC in a TLS-55 rotor (Beckman),
  10. Crystal Structures of Bacillus subtilis Lon Protease

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/londumanjmb2010.pdf
    20 Jul 2010: the protease domainof Lon is a 150- to 200-amino-acid-long, highlyconserved region located at the C terminus.
  11. A Large Particle Associated with the Perimeter of the ...

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb82.pdf
    5 Jul 2010: 3). Clearly visible are : (a) "rings" which have an insidediameter close to 800 A and an outer diameter the same as thatofthe pore complex itself(- 1,200 A) ; (b) large particles

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