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8 Feb 2008: a linear, additive fashion[16,17], with typical increases in stability of around 1–2 kcal/mol in 200–300 g/L of cosolute [16,18–22]. ... Examination ofdestabilised mutants of ubiquitin here confirms this hypothesis,with an increase around 1.2

8 Feb 2008: at pH 7.5 in a buffer containing 200 mM KCl and10% (v/v) glycerol. ... Conditions: 25 C, 50 mM Tris–HCl(pH 7.5), 200 mM KCl, 10% (v/v) glycerol, 1 mM DTT.

8 Feb 2008: Forlocalisation of hydrogen exchange in pepsin-digestedHsp90 samples, typical instrumental settings include:mass range 200–2000 Da, capillary voltage 1400 V andcone voltage 20 V.

8 Feb 2008: A 200 μl sampleof 20 μM DMAG was injected into a 1.4 ml cell containing1 μM wild-type Hsp90.

4 Jun 2008: Bound protein was eluted witha linear salt gradient over 200 mL from 0 to 2 M NaCl.Protein was further purified by gel-filtration chromato-graphy on either a G75 Sepharose ... Protein interaction assays. Protein interaction was assayed according to

1 Aug 2008: from the amino terminus, as the threading of an Escherichia. coli ribosome (approximately 200 Å in diameter [Schuwirth. ... 50 mM Tris-HCl (pH 7.5), 200 mM KCl, 10 % (v/v) glycerol, and 1 mM DTT at.

8 Feb 2008: 225 nm. Conditions: 25C in 50 mM Tris-HCl. (pH 7.5), 200 mM KCl, 10% (v/v) glycerol,. ... 200 mM KCl, 10 % glycerol (v/v), 1 mM DTT, except for the ITC exper-.

1 Aug 2008: Structure 16, May 200. to be a compact state in which the N-ter-. ... require large quantities of stable and. homogeneous species. Structure 16, May 200.

8 Dec 2008: All experiments were performed in abuffer of 50 mM TrisHCl (pH 7.5), 200 mM KCl, 10% glycerol (vol/vol), 1 mMDTT, except for ITC experiments where -mercaptoethanol replaced DTT asthe