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"Rational Design and Evaluation of FRET Experiments to Measure…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/40_Snapp_EL_Curr_Prot_2006.pdf11 May 2007: Consider that a homogeneouslydistributed fluorescent protein must be presentat 200 nM to be visualized over backgroundcellular fluorescence (Niswender et al., 1995).Thus, experimental questions involving cellu-lar expression of a fluorescently -
Alonsoetal Supplementary Material 030107
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Alonso-suppmat.pdf2 Apr 2007: buffer at 200 V for 1.5 hours. -
CELL AND MOLECULAR PHYSIOLOGY Intracellular Ca2+ release via the ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/44_Ong_HL_Pflug_Arch_2007.pdf15 Feb 2007: Lysatesfrom HSG cells were then prepared as described previously[30]. Briefly, whole cell lysates were centrifuged through a10–50% sucrose gradient and 200 μl fractions weresequentially removed from the centrifuged lysate. ... The indicated protein -
Contents of Supplementary Figures Supplementary Figure 1:…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/DaumkeMcMahonEHDNature/Daumke_McMahon_EHD_Sup.pdf24 Sep 2007: 66. : 58. : 139. : 68. : 200. α1a β1. • • • • • ... at 10 C in 100 mM HEPES (pH 7.5), 50 mM NaCl, 200 µM CaCl2. -
DOI: 10.1126/science.1135394 , 239 (2007); 317Science et al.Nabil…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17626883.pdf8 Dec 2007: 150. 200. WT E542K E545K Q546K- - - - p85ni. p110 α. ... 0. 50. 100. 150. 200. 250. - - Phosphopeptide. C. B. -
DOI: 10.1126/science.1136985 , 120 (2007); 316Science et al.Maria C.…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Alonso_Science.pdf6 Apr 2007: on. Apr. il 6,. 200. 7 w. ww. sci. ence. mag. ... REPORTS. on. Apr. il 6,. 200. 7 w. ww. sci. ence. -
doi:10.1016/j.jmb.2007.08.056
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Oliva_FtsZ_JMB_2007.pdf11 Oct 2007: After precipitation in 30% ammonium sulfate saturationand anion-exchange chromatography, the protein wasdialyzed against 50 mM Tris–HCl (pH 8.0), 200 mM KCl,1 mM EDTA, 10% (v/v) glycerol ... Drops were composed of100 nl of protein at 10.0 g/l (with 8 -
doi:10.1016/j.str.2007.05.002
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/FCH02F-BARHenne07.pdf2 Jun 2007: a Superdex 200 column; 130 mM mFCHo2 (res-. idues 1–261) was loaded. ... H) Control Folch fraction 1 liposomes without addition of protein. The scale bars represent 200 nm. -
ESMS of membrane proteins
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/methods/ESMS%20of%20membrane%20proteins.pdf20 Jul 2007: mix - add 200 μl water, mix vigorously - centrifuge 2 min 10.000x g - take top phase off ( 500 μl), protein forms precipitated layer - add 300 μl methanol - centrifuged 1 min -
Interaction of tau protein with the dynactincomplex Enrico…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/EMBOJ2007-Magnani-Fan.pdf15 Oct 2007: pred. ictio. n. Arpl binding0. 0.0. 0.5. 1.0. 200 400 600 800 1000 1200.
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