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CELL AND MOLECULAR PHYSIOLOGY Intracellular Ca2+ release via the ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/44_Ong_HL_Pflug_Arch_2007.pdf15 Feb 2007: Lysatesfrom HSG cells were then prepared as described previously[30]. Briefly, whole cell lysates were centrifuged through a10–50% sucrose gradient and 200 μl fractions weresequentially removed from the centrifuged lysate. ... The indicated protein -
Alonsoetal Supplementary Material 030107
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Alonso-suppmat.pdf2 Apr 2007: buffer at 200 V for 1.5 hours. -
DOI: 10.1126/science.1136985 , 120 (2007); 316Science et al.Maria C.…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Alonso_Science.pdf6 Apr 2007: on. Apr. il 6,. 200. 7 w. ww. sci. ence. mag. ... REPORTS. on. Apr. il 6,. 200. 7 w. ww. sci. ence. -
"Rational Design and Evaluation of FRET Experiments to Measure…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/40_Snapp_EL_Curr_Prot_2006.pdf11 May 2007: Consider that a homogeneouslydistributed fluorescent protein must be presentat 200 nM to be visualized over backgroundcellular fluorescence (Niswender et al., 1995).Thus, experimental questions involving cellu-lar expression of a fluorescently -
tra_578 1..18
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Burtey%20IVF%20Traffic%2007.pdf15 May 2007: # 2007 The Authors. doi: 10.1111/j.1600-0854.2007.00578.xTraffic 2007Blackwell Munksgaard. The Conserved Isoleucine–Valine–PhenylalanineMotif Couples Activation State and EndocyticFunctions of b-Arrestins. Anne Burtey1,2, Eva M. -
doi:10.1016/j.str.2007.05.002
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/FCH02F-BARHenne07.pdf2 Jun 2007: a Superdex 200 column; 130 mM mFCHo2 (res-. idues 1–261) was loaded. ... H) Control Folch fraction 1 liposomes without addition of protein. The scale bars represent 200 nm. -
Structural insight into the ESCRT-I/-II link and itsrole in ...
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17215868.pdf14 Jun 2007: 0 50 100 150 200. 0.22. 0.27 27 nM. 61 nM. ... Superdex 200 10/300 column. 16 20128. 6 10 14 18Elution volume (ml). -
Liposome and Lipid tubule Production
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/Liposome.html25 Jun 2007: NB: the filtration process usually leads to loss of around 100-200 ul of liquid, and 10-20% of the lipid in the reamining volume. -
Negative-staining protocol for EM: Harvey McMahon lab
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/Negative%20stain%20protocol.html25 Jun 2007: proteins, or complexes smaller than 200 kDÑin my hands, GroEL 14mers (840kD) or clathrin triskelia (600 kD) can be made out on a good grid but HSP60 monomers cannot). -
Structures of endocytic proteins
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/research/structur/structure.html27 Jun 2007: Gallery of structure for endocytic proteins. There are also links to pages for many endocytic and trafficking proteins.
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