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Graeme Mitchison Publications List - MRC Laboratory of Molecular…
https://www2.mrc-lmb.cam.ac.uk/graeme-mitchison-publications-list/21 Jul 2023: B. Limit to the detection of Glass patterns in the presence of noise. ... Bounds on the learning capacity of some multi-layer networks. Biol. Cybern. -
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/12045
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/12045{"id":12045,"date":"2018-05-08T14:55:59","date_gmt":"2018-05-08T13:55:59","guid":{"rendered":"https://www2.mrc-lmb.cam.ac.uk/?page_id=12045"},"modified":"2023-07-21T12:53:50","modified_gmt":"2023-07-21T11:53:50","slug":"graeme-mitchison-publications- -
Uli Arndt Publications List - MRC Laboratory of Molecular Biology
https://www2.mrc-lmb.cam.ac.uk/uli-arndt-publications-list/21 Jul 2023: 1954) Some X-ray Scattering Results on Nucleoproteins and their Components Trans Faraday Soc. ... Arndt, U.W. & Riley, D.P. (1955) The Structure of Some Proteins as Revealed by an X-ray Scattering Method Phil. -
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/21949
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/21949{"id":21949,"date":"2021-12-20T14:38:57","date_gmt":"2021-12-20T14:38:24","guid":{"rendered":"https://www2.mrc-lmb.cam.ac.uk/?page_id=21949"},"modified":"2023-07-21T12:51:48","modified_gmt":"2023-07-21T11:51:48","slug":"from-the-scientists-view-a-con -
Mark Bretscher's Publications List - MRC Laboratory of Molecular…
https://www2.mrc-lmb.cam.ac.uk/mark-bretschers-publications-list/21 Jul 2023: Thomson and B.M.F. Pearse. It is shown that some surface proteins are excluded from plasma membrane coated pits as they bud into the cell. ... A substratum was prepared to which the transferrin receptor could specifically attach; motile cells were able -
Dan Brown's Publications List - MRC Laboratory of Molecular…
https://www2.mrc-lmb.cam.ac.uk/dan-browns-publications-list/21 Jul 2023: Some heterocyclic analogues of stilbenes. J. Chem. Soc., 1948, 2147-2154. D M Brown &B Lythgoe. ... Nucleotides. Part XXIV. The action of some nucleases on simple esters of monoribonucleotides. -
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/2034
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/pages/2034 -
From the scientist’s view: a conversation with … John Sutherland -…
https://www2.mrc-lmb.cam.ac.uk/life-at-the-lmb/profiles/from-the-scientists-view-a-conversation-with-john-sutherland/21 Jul 2023: and it was also an opportunity to say farewell to some of the things about universities which I found frustrating; the underfunding and the administrative chores in universities. ... I’m quite happy with the ones we have made. I would like to make some -
Model Building and Refinement Practical Introduction In this…
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/tutorial/ed-lowe-mb.docx27 Apr 2016: 2.2.4.4. The model now looks like it fits the map a great deal better, but there is still some room for improvement so we will carry interactively refine ... 3.1.4. Leave fields “Phases”, TLS coefficients” and “Reference model” may be left as -
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/posts/23599
https://www2.mrc-lmb.cam.ac.uk/wp-json/wp/v2/posts/23599{"id":23599,"date":"2022-06-29T14:12:52","date_gmt":"2022-06-29T13:12:52","guid":{"rendered":"https://www2.mrc-lmb.cam.ac.uk/?p=23599"},"modified":"2022-06-29T14:12:52","modified_gmt":"2022-06-29T13:12:52","slug":"lister-institute-prize-lectures-deli -
doi:10.1016/j.jmb.2004.05.031
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/zapa%202004.pdf16 Aug 2004: ZapA is pre-dominantly dimeric (MW 23,612 Da) although some tetramer (MW 52,619 Da) exists at this concentration.D, Increasing ZapA concentration (2.5 mg/ml (A280 0.75)) ... B, Confirmation of stoichiometric relationshipobserved in A: titration of ZapA -
Crystal Structure of the SMC Head Domain: An ABC ATPase with 900…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/TmSMChd%20JMB%202001.pdf20 Feb 2001: 1998). Chromo-some dynamics: the SMC protein family. Curr.Opin. Genet. Dev. 8, 254-259. ... Mol.Microbiol. 17, 1215-1217. Strunnikov, A. V. (1998). SMC proteins and chromo-some structure. -
Twinning and other pathologies Andrey Lebedev CCP4 OD-‐structures…
https://www2.mrc-lmb.cam.ac.uk/download/twinningaandaotherapathologiesa-andreyalebedeva-a/?wpdmdl=18788&refresh=6681665f5eb75171975638311 Jun 2013: Twins by reAcular merohedry (inc some OD-‐twins), allotwins, disordered structures -‐ Can be readily seen in diffracAon images (with spot predicAons shown). ... Molecular Replacement: –Two structures are globally very similar (e.g. rmsd = 0.5A) -
mbar-v2
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/tutorial/model-building-and-refinement-v2.pdf31 Aug 2018: 1.2.4.4.The model now looks like it fits the map a great deal better, but there is still some room for improvement so we will carry interactively refine ... In some cases this can be very helpful. 1.2.7.6.His 17 has been built without a side chain, but -
mmi_4133.fm
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Spo0J%202004.pdf30 Jun 2004: et al. , 2002).Identification and characterization of a bacterial chromo-some partitioning site revealed 10 pseudopalindromic16 bp sequences found in the origin-proximal 20% of the. ... Res. idua. l (D. 23. 0). SpoOJ C-terminus (Spo0J223-269). A B. -
doi:10.1016/j.biocel.2008.08.010
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/cytomotive2008Lowe.pdf17 Sep 2008: Almost certainly, cells were able to divide, by some unknown. eans, before this protein fold was perfected. ... BtubAB was found in some deep-branching VerrucomicrobiaJenkins et al., 2002) and forms longitudinal protofilaments withlternating A and B -
No Job Name
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/vandenent2008ftsq.pdf4 Mar 2008: Despite the sequential dependency in which the celldivision proteins seem to localize, some of the compo-nents of the divisome can form a complex before theyreach the septum independent of their ... It has been proposed that some of theexposed edges of -
Features critical for membrane binding revealed by DivIVA crystal…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/emboj201099a.pdf20 May 2010: Full-length protein is a tetramer, although some non-reversible aggregation also occurred. ... Full assignment is shown. (B) Summary of NMR backbone dynamics showing secondary structure elements and alsothat some residues at the C-terminal end are not -
Refined Structure of (alpha)(beta)-Tubulin at 3.5 A Resolution
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/jmb%20tubulin%20refinement.pdf3 Nov 2001: Consequently residues 35-60 are not included in the model of the a-tubulin.Some improvement was, however, visible for the. ... Generally residues involved directly in nucleo-tide binding and hydrolysis are highly conservedbetween a and b-subunits, with -
Lister Institute Prize Lectures delivered at LMB - MRC Laboratory of…
https://www2.mrc-lmb.cam.ac.uk/lister-institute-prize-lectures-delivered-at-lmb/29 Jun 2022: an individual, defining the process by which new variants likely arise, and also reporting some of the first data on Pfizer BioNTech vaccine-induced antibody responses against the ... These G1 macrophages are highly vulnerable to HIV-1 infection. -
The ER membrane protein complex is a transmembrane domain insertase
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017c.pdf27 Jan 2018: In some experiments, nucleased RRL was used. Here, CaCl2 is added to 1 mM and the Ca2+-activated nuclease from S. ... In some reactions, a tripeptide competitive inhibitor of glycosylation (Asn-Tyr-Thr) was added at 50 µM to verify identity of the -
doi:10.1016/j.tibs.2006.08.004
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/42_Bernstein_HD_TIBS_2006.pdf17 Aug 2006: 39]. As a result, a fraction of some proteins (e.g. hepatitis B virus precore and. ... b,c) The cleaved signal peptides of some viral. glycoproteins mediate functions that are essential for the viral life cycle. -
Crystal Structure of a Calcium-Phospholipid Binding Domainfrom…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9430701.pdf20 Apr 2002: In addi-tion to calcium, the activation of cPLA2 by some agonists re-quires phosphorylation of the enzyme (7, 8). ... The model showed that the molecular replacement solution hadcorrectly placed the b-sheets, but some loop conformations, -
PII: S0962-8924(99)01504-4
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/14_Hegde_RS_TCB_1999.pdf11 Mar 1999: In the case of some TM domains, the core translo-con might suffice2,25. ... Thus, thecurrently mysterious normal function of PrP, as wellas development of some of its associated diseases35,might be regulated at the level of protein biogenesisand topology. -
Cell, Vol. 92, 621–631, March 6, 1998, Copyright 1998 ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/12_Hegde_RS_Cell_1998.pdf28 Feb 1998: cross-linker.served some role in maintaining ribosome binding, then(B) Nascent chain translocation intermediates of pPL-86-mer, pPL-. ... While this still may be the supported by the observation that metabolic conditionsunder which apo B is being -
Membrane Localization is Critical for Activation of the PICK1 BAR…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/MadsenGetherTraffic2008.pdf18 Oct 2008: PICK1 was distributed equally between the two fractions,. suggesting some constitutive membrane association of. ... some intracellular, vesicular accumulation (Figure 5A). However, upon cotransfection with PICK1, we observed. -
Nicotinic Acetylcholine Receptor at 4.6 Å Resolution: Transverse…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jmb99.pdf15 Sep 2003: A general property of tubular crystals is thatthere is some variation in the helical pitch (ororientation of the surface lattice), even within agiven helical family. ... There are crescents of about seven suchpeaks and some additional tunnel-lining -
refmac 5.8 series
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/refmac/bug_fixes.html6 Aug 2014: 5.8.0036. Symmetry related restraints. There were some problems with symmetry related external restraints. ... Map sharpening would produce noisy map in some cases. Now sharpening has two components: 1) Removable B value and 2) regularised sharpening B -
Crystal Structures of Bacillus subtilis Lon Protease
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/londumanjmb2010.pdf20 Jul 2010: dependent protease FtsH, and thus, Lonproteases of the Lon B type fulfil their roles in theseorganisms.22 Some bacteria, such as Thermotogamaritima, Pseudomonas aeruginosa, and Bacillus sub-tilis, express both Lon ... Some of theseinteractions are of a -
Prokaryotic cytoskeletons: protein filaments organizing small cells
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nrmicro.2017.153.pdf18 Jan 2018: Division in some cases, others unknown. 14,142,143. CrvA Bacteria Vibrio spp. ... 105,152. PopZ Bacteria Some Gram-negative bacteria. Cell pole marker, signalling hub. -
Molecular Cell, Vol. 2, 85–91, July, 1998, Copyright 1998 ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/13_Hegde_RS_MolCell_1998.pdf16 Jul 1998: However, secPrP and NtmPrPwere made efficiently in only some of the membranepreparations, showing optimal translocation at 0.5%and 0.45%, respectively. ... minimal machinery necessary for translocation (SRP re-ceptor, Sec61 complex, and in some cases TRAM -
PII: S0304-3991(97)00070-3
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ultram97.pdf4 Apr 2003: Membrane proteins and also some soluble proteins form these crystals through protein-protein interactions or through. ... parameters. Clearly there may be some advantage in repeating the overall procedure using the distortion-corrected structure as a new -
Protein-driven membrane stresses in fusion and fission
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/KozlovCurvature2010.pdf21 Feb 2011: However, the fusion peptideis clearly more than just a hold on a target membrane,because some amino acid substitutions within the fusionpeptide inhibit fusion without lowering peptide–mem-brane binding. ... membrane stresses, has been confirmed in some -
GB-9-7-229-Amos_9.qxd
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/gb-2008-9-7-229.pdf4 Aug 2008: outer doublet microtubules (Figure 3a-f), some questions. remain about their exact locations and functions. ... Genome BBiioollooggyy 2008, 99::229. It would also be interesting to know what makes some. -
Dynamic Filaments of the Bacterial Cytoskeleton
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/annrev2006.pdf19 Apr 2006: FtsZ isalso present in some chloroplasts and mito-chondria (17, 18). FtsZ in vivo. ... Some bacteria contain several re-lated MreB-like genes. For example B. sub-tilis encodes MreB, Mbl, and MreBH, witheach one showing a similar degree of -
McMahon Group publications
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/papers.html20 Mar 2023: some interaction partners. ... In some of these proteins (N-BARs) the BAR is adjacent to an N-terminal amphipathic helix, and these N-BAR proteins can drive membrane curvature in vitro and in vivo. -
Amos&Schlieper.indd
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Amos%26Schlieper.pdf16 Nov 2005: Perfect helical symmetry (with all lateral interac-tions alike) is possible for B-lattice microtubules with some pf numbers, such as 12, 15 or 16, but a standard 13-pf microtubule ... as a narrow sheet for some distance beyond the end of the closed tube. -
Qrb1300006 1..40
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/qrb13.pdf30 Jul 2013: of their development. Although in some aspects uniquely suited to the Torpedo system, these. ... seen extending over some vesicles (Fig. 1b). Elongated vesicles and short poorly ordered tubes,. -
REPORT◥ PROTEIN HOMEOSTASIS Mechanistic basis for a moleculartriage…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017.pdf19 Jan 2017: S12). This suggests that TA protein releasedfrom SGTA is primarily recaptured by SGTA, un-less a high local concentration of BAG6 interactingwith SGTA permits some capture by BAG6. ... transfer to TRC40 is fast, some loss seems in-evitable. Consistent -
Cooperative Recruitment of Dynamin and BIN/Amphiphysin/Rvs (BAR)…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/JBC_Meinecke2013.pdf19 Mar 2013: Control samples weretransfected in the same way as the RNAi samples but a scrambledcontrol siRNA oligo (Invitrogen) was used instead.In some experiments, cells were transfected using. ... The addition of GTP to membrane-bound dynaminresulted in some -
JCB: Article The Rockefeller University Press $30.00J. Cell…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/63_Rane_NS_JCB_2010.pdf16 Feb 2010: This, however, posed some ex-perimental limitations (hence the reason for initially focusing on the more tractable AV3 mutant). ... Some HuPrP(A117V)36 mice also show kyphosis (hunched posture; arrow). (B) The rough hair coat in HuPrP(A117V)36 mice, -
Tyrosine Residues in Phospholipase C�2 Essential for the…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/11606584.pdf20 Apr 2002: Some of the additional sites couldbe within the 2SA protein (containing 24 tyrosine residues), assuggested in Fig. ... These (Tyr771,Tyr783, and Tyr1254) and some additional sites have been iden-tified after in vitro phosphorylation of purified PLC1 by -
pnas201210899 16522..16527
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2012-Aylett-16522-7.pdf12 Oct 2012: BmTubR (UniProt Q848W2_BACMQ) lies within an operon con-taining a TubZ homolog in B. ... Proposed ModelBm TubR-DNA. Crystal StructureBt TubR-tubC. Proposedtopological. rearrangement. A B. C D. -
The GTPase-Activating Protein GRAF1 Regulates the CLIC/GEEC Endocytic …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Lundmark_McMahon_GRAF2008.pdf28 Nov 2008: Although GRAF1 can be found primar-ily on long tubules in some cells, other cells within the samepopulation exhibit a predominantly punctate GRAF1 localiza-tion. ... The depicted images are used to show some of the different morphologies of -
Quality and quantity control at the endoplasmic reticulum
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/65_Hegde_RS_COCB_2010.pdf7 Jun 2010: B) Lumenal substrates and some membrane proteinsaccess the catalytic site by a translocation-dependent mechanism. ... Indeed, some systems may recruitmachinery that is normally not even used for misfoldedproteins (such as bTrCP). -
doi:10.1016/j.cell.2006.03.035
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/16647110.pdf14 Jun 2006: B) Distribution of IC50 values for selected inhibitors against PI3-K family members. ... Some IC50 values for AMA-37 and TGX-115 have been reported. previously (Knight et al., 2004). -
A substrate-specific inhibitor of proteintranslocation into the…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/35_Garrison_JL_Nature_2005.pdf15 Jul 2005: 3a, b, and data not shown).Immunoprecipitation studies identified the primary differences(Fig. ... Some of the major depleted proteins are indicated (open arrows). b, As in a, except that samples were analyzed by immunoblotting with PDI , Sec61α , TRAM , -
doi:10.1016/j.jmb.2005.02.053
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/34_Menetret_JF_JMB_2005.pdf6 Apr 2005: Separate resolution curves for the ribo-some and the channel were then calculated from theappropriate volumes. ... The resolution of this map is w14.7 Å(FSC0.5, Figure 1(b)) and some surface-exposedrRNA features are lost due to interactions -
Evolving nature of the AP2 a-appendage hubduring clathrin-coated…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/AP2Hub_EMBO2004/AP2Praefcke2004.pdf23 Nov 2004: B) We found that NECAP does not bind to a-F740Dand thus we probed some mutants around this site along with the top site mutant W840A. ... It is. probable that for steric reasons only some of these motifs can. -
Dissertation
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/AndyHuang.pdf6 Mar 2008: For measuring some features such as body length, body width, velocity and angle. ... during image capture and processing. Some of these features are described in detail:.
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