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  2. Lab protocols | Ron Lab

    https://ron.cimr.cam.ac.uk/lab-protocols
    23 Feb 2024: Search site. Ron Lab. Lab protocols. Lab protocols. Lab protocols. Protocols related to mutant mice. Whilst we no longer maintain stocks of live mice, most of the strains made by the lab over the years have been cryopreserved as embryos and are

  3. January 3, 2001Detection of Phospho-eIF2alpha:1. For most cell types…

    https://ron.cimr.cam.ac.uk/files/p3_eif2ap.pdf
    13 Oct 2021: January 3, 2001Detection of Phospho-eIF2alpha:1. For most cell types use dishes which are less than 95%. conflulent. For cells with a high metabolism such as ES cells or 293Tcells use 50-60% confluent dishes. 2. Change the media 1-2hrs before

  4. The absence of nuclear eIF2 Background Eukaryotic translation…

    https://ron.cimr.cam.ac.uk/system/files/nonucleareif2a.pdf
    NoNucleareIF2a.pdf 11/24/02. Discussion. We were unable to detect eIF2α within the nuclei of two cell lines. ... 2001 Aug 10;293(5532):1139-42. Stefan Marciniak November 2002. email: marcinia@saturn.med.nyu.edu. NoNucleareIF2a.pdf 11/24/02.

  5. Commentaries and postings | Ron Lab

    https://ron.cimr.cam.ac.uk/commentaries-and-postings
    23 Feb 2024: Search site. Ron Lab. Commentaries and postings. Commentaries. Commentaries and Postings from the Ron Lab. 2024 University of Cambridge.

  6. Publications | Ron Lab

    https://ron.cimr.cam.ac.uk/publications
    23 Feb 2024: elegans. Proc Natl Acad Sci U S A 105:7094-7099 Download pdf. ... Download pdf Also see commentary by Rutkowski & Kaufman. "All Roads Lead to ATF4": Dev Cell 4:442-4 (2003).

  7. PERK or GCN2 IP Western In our experience, the ...

    https://ron.cimr.cam.ac.uk/files/protocol_1_perk_or_gcn2_ip_western.pdf
    12 Oct 2020: PERK or GCN2 IP Western In our experience, the detection of PERK phosphorylation (or IRE1 phosphorylation) as markers of ER stress is fraught with great difficulties. None of the anti-phosphoPERK antisera we have tested are able to detect the

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