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A Conserved Archaeal Pathway for TailAnchored Membrane Protein…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/70_Sherrill_J_Traffic_2011.pdf11 Nov 2011: We also examined whether archaeal TRC40 could func-tionally replace the endogenous mammalian TRC40 atphysiological concentrations. ... absence of endogenous mam-malian TRC40 using a phenyl- and Diethylaminoethyl (DEAE)-Sepharose-depleted RRL (PD-RRL) -
MTP V _forges davanzati_
https://www.robinson.cam.ac.uk/postkeynesian/downloads/Forges/GFD210513.pdf26 Apr 2013: Graziani constructed a basic schema of the working of a monetary economy (see below – section 2), based on the idea that money supply is endogenous and demand-driven. ... These theoretical elements enter the basic schema of the MTP, with particular -
25 Structural studies of phosphoinositide 3-kinase-dependent traffi c …
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17233579_proof.pdf14 Jun 2007: How do various ESCRT components link to other processes such as pH regulation of gene expression [51] or retrovirus budding [52]? -
JFCregg_OGS_finaledit
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/Cregg_thesis.pdf14 Dec 2010: amount of endogenous ACh at the synapse by preventing its metabolism by AChE. ... communication). 8. The endogenous agonist, serotonin, is a stable compound but was thought not. -
Science Journals — AAAS
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2023/08/Science_2021.pdf26 Aug 2021: Othersubunits of the 19S base were copurified atlower levels, consistent with partial assemblyof PSMC5 with endogenous base subunits pres-ent inthe lysate (table S5). ... Boosting HERC1 levelsusing CRISPR activation (31) of the endoge-nous HERC1 promoter -
The structure of human thyroglobulin
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/TG_Nature2020.pdf31 Jan 2020: rTG appears to be indistinguishable by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and cryo-electron microscopy (cryo-EM) from endogenous TG (eTG) puri-fied from thyroid glands of patients -
JCB: Article The Rockefeller University Press $30.00J. Cell…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/63_Rane_NS_JCB_2010.pdf16 Feb 2010: 2007). To maximize the severity of any phenotype, PrP+/ mice were used because endogenous PrP was observed in earlier studies to exacerbate CtmPrP-mediated neurodegeneration (Stewart et al., 2005). -
Molecular Biology of the CellVol. 19, 3463–3476, August 2008 ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/51_Ashok_A_MBC_2008.pdf16 Jul 2008: SDS-PAGE, andimmunoblotted using an antibody (3F4) that specifically detects transfected but not endogenous PrP. ... C) Biosynthesisand maturation of endogenous PrP in A4 and L-cells were assessedby pulse-chase analysis followed by immunoprecipitation -
Qrb1300006 1..40
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/qrb13.pdf30 Jul 2013: inherent flexibility of the tubes and other factors, such as heterogeneity of the endogenous. -
Articleshttps://doi.org/10.1038/s41594-019-0196-z 1MRC Laboratory of…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Burmann%202019%20NSMB.pdf4 Mar 2019: Articleshttps://doi.org/10.1038/s41594-019-0196-z. 1MRC Laboratory of Molecular Biology, Cambridge, UK. 2Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, UK. 3Bioanalytics, Institute of Biotechnology, Technische -
elifesciences.org van den Ent et al. eLife 2014;3:e02634. DOI: ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mrebdouble2014.pdf6 Jun 2014: elifesciences.org. van den Ent et al. eLife 2014;3:e02634. DOI: 10.7554/eLife.02634 1 of 22. Bacterial actin MreB forms antiparallel double filamentsFusinita van den Ent1†, Thierry Izoré1†, Tanmay AM Bharat1, Christopher M Johnson2, Jan Löwe1. -
Protection from cytosolic prion protein toxicityby modulation of…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/30_Rane_N_EMBO_2004.pdf8 Nov 2004: Endogenous NF-kB (indicated by an aster-isk) serves as a loading control. ... Immunoblots were probed with antibodiesagainst PrP, GFP, and an endogenous protein, the beta subunit ofSec61. -
doi:10.1016/j.cub.2004.07.058
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/15296756.pdf6 Oct 2004: The challenge now is to establish the molecularhave similar phenotypes: they retain a similar shape tolinks between endogenous ARAP3’s control of Rho andcontrol cells, but on PDGF stimulation their lamellipodiaArf -
Chapter 20 In Vitro Dissection of Protein Translocationinto the ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/62_Sharma_AS_MMB_2010.pdf30 Apr 2010: Itdoes, however, stimulate the translation of some proteins(presumably because the endogenous levels of some tRNAsin the RRL are limiting), and it is possible that some RRLpreparations contain relatively low amounts ... 5. Endogenous RRL usually contains -
JCB: Article The Rockefeller University Press $30.00J. Cell Biol. ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/J%20Cell%20Biol%202010%20Howes.pdf24 Aug 2011: By Western blot analysis both endogenous and transfected Flot1 and GRAF1 were concentrated in Fraction 2.8 (Fig. ... 4 G, arrowheads). Endogenous dynamin labeled a significant population of struc-tures within Fraction 2.8 (Fig. -
15591370691369 1..30
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/elife_2019.pdf29 May 2019: For correspondence:. rhegde@mrc-lmb.cam.ac.uk. Competing interest: See. page 28. Funding: See page 28. Received: 11 March 2019. Accepted: 15 May 2019. Published: 16 May 2019. Reviewing editor: Maya. Schuldiner, Weizmann Institute,. Israel. Copyright -
Ribosome-associated protein quality control
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/98_Brandman_O_NSMB_2016.pdf18 Feb 2016: Ribosome profiling in the presence and absence of Dom34 has shown that an endogenous substrate for this pathway is Hac1, a cytosolically spliced mRNA whose incorrect ligation results in an mRNA ... The key open questions include the following: (i) What -
4380 | G. J. Doherty et al. Molecular Biology ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Doherty2011/DohertyGRAF_MBC2011.pdf17 Jan 2012: Instead, both endogenous and overexpressed GRAF1 were found enriched at small, round, vincu-lin-positive structures in a subset of cells (see Figure S1, C and D). ... Cells were acid-stripped and fixed and were then labeled for endogenous paxillin. -
Molecular Biology of the CellVol. 19, 5093–5103, December 2008 ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/LynchMBC5093.pdf24 Jan 2009: It remains tobe determined whether endogenous Syt-1 regulates dense-core vesicle fusion pore expansion and if so, whether Syt-1utilizes membrane binding and curvature induction, or in-teractions with SNAREs, ... Syt Effector Interactions Regulate Fusion -
TTC5 mediates autoregulation of tubulin via mRNA degradation
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2019.pdf19 Nov 2019: Cite as: Z. Lin et al., Science 10.1126/science.aaz4352 (2019). REPORTS. First release: 14 November 2019 www.sciencemag.org (Page numbers not final at time of first release) 1. Alpha and beta tubulins form obligate heterodimers (hereaf-ter -
OWN-RATES SRAFFA & KEYNES CH17-3(1)(4)
https://www.robinson.cam.ac.uk/postkeynesian/downloads/Grieve/RG250214.pdf11 Feb 2014: 1. 04/09/04/02 An issue with own-rates: Keynes borrows from Sraffa , Sraffa criticises Keynes,. and present-day commentators get hold of the wrong end of the stick. Roy H Grieve1. Abstract. Scholars who in recent years have studied the Sraffa papers -
ELIFE07975 1..21
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/94_Voorhees_RM_eLife_2015.pdf11 Aug 2015: By contrast, endogenous SRP in a homologous mammalian system hasa remarkably subtle effect on translation elongation (Wolin and Walter, 1989). ... Here we demonstrate that endogenous SRP is efficiently recruited to mammalian ribosomescontaining a -
Are there policy alternatives to Ireland’sausterity? Stephen Kinsella …
https://www.robinson.cam.ac.uk/postkeynesian/downloads/Kinsella/SK191113S.pdf19 Nov 2013: I Steady state solution, simulation, policy experiments. I Generally post-Keynesian features, effective demand,endogenous money, markup based pricing, proceduralrationality, etc. -
Estere Seinkmane – November 2018 1 5. Metabolism throughout ...
https://www.murrayedwards.cam.ac.uk/sites/default/files/files/Activity%205_%20Metabolism%20throughout%20the%20day.pdf5 Feb 2019: and Scheer, F. Endogenous circadian system and circadian misalignment impact glucose tolerance via separate mechanisms in humans. ... The circadian clock is an endogenous biological mechanism that drives roughly 24h cycles in various physiological -
The patterned assembly and stepwise Vps4-mediated disassembly of…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Hurtig%202023%20sciadv.ade5224.pdf16 Jul 2023: 1A), hereafter calledVps4E209Q. This point mutation prevents AAA-ATPases like Vps4from hydrolyzing bound ATP and can therefore interfere with theactivity of endogenous Vps4 complexes when overexpressed (24,28). -
Mechanism of ribosome stalling during translation of a poly(A) tail
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/NSMB_2019.pdf25 Nov 2019: Nature Structural & Molecular Biology, doi:10.1038/s41594-019-0331-x -
Molecular Biology of the CellVol. 21, 3054 –3069, September ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/RVS161_MBC2010.pdf21 Feb 2011: Previous characterization ofBAR domain proteins has generally involved overproduc-tion, raising the need for in vivo studies characterizing theconsequences of BAR domain mutations in genes expressedat their endogenous level. ... these alterations affect -
DOI: 10.1126/science.1256917, 963 (2014);346 Science et al.Thomas G.…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Science-2014-Gligoris-963-7.pdf21 Nov 2014: Toaddress whether cohesin containing Smc3R61Qloads onto chromosomes, we compared the dis-tribution of green fluorescent protein (GFP)–tagged Smc3R61Q and wild-type Smc3 in livingcells carrying an untagged endogenous Smc3gene. -
articles Nucleotide-dependent conformational changes in dynamin:…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Stowell_McMahon1999.pdf30 Apr 1999: and endogenous rat brain vesicles. J. Biol. Chem. 268, 17240–17246 (1993). -
Membrane Localization is Critical for Activation of the PICK1 BAR…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/MadsenGetherTraffic2008.pdf18 Oct 2008: a high concentration of an endogenous PDZ-binding. Figure 5: The C-termini of GluR2 and of DAT fused to thesingle transmembrane protein Tac are sufficient for BARdomain-dependent coclustering with ... This. juxtanuclear clustering strongly resembles the -
Identity Politics and Trade Policy Gene M. GrossmanPrinceton…
https://www.christs.cam.ac.uk/sites/default/files/inline-files/Slides_Identity_Politics_Mead_022719_handout.pdf27 Feb 2019: Focus on the level of protectionStudy endogenous shifts in self categorization triggered by changes ineconomic conditions (e.g., rising income inequality due to trade ortechnology) or by political opportunism that accentuates ... differencesSelf -
Deubiquitinases Sharpen Substrate Discrimination during Membrane…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/82_Zhang_ZR_Cell_2013.pdf5 Aug 2013: Second, the system is typicallyorthogonal to endogenous pathways and utilizes a single definedligase. ... Furthermore, the absolute dependence on phospho-Vpu illustrates that endogenous ligases in this system do notperceive mCD4 as a misfolded protein -
3747_0_supp_7_1052456676
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/christian.pdf9 May 2003: endogenous proteins and quenching of signals by other cellular proteins. This discussion. -
PII: S1534-5807(04)00100-5
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Johannes_DevCell2004.pdf22 Mar 2005: Bar 10 m. endogenous MPR300 and TGN46 were relocalized to these conditions was not addressed directly. ... In agreement with these observations, we foundthat the endogenous EGF receptor was not relocalizedmembranes. -
bst641-09a
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/19614567.pdf2 Aug 2010: 26]. The p101 subunitprotects cells from apoptosis when overexpressed, and thismay explain why it is a common site for insertion in T-celllymphomas induced by the MoFe2-MuLV retrovirus [27].The -
Molecular Biology of the CellVol. 8, 2003–2015, October 1997 ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/WiggeKohler_McMahon1997.pdf10 Oct 1997: We interpret these results tomean that either Amph1 or Amph2 overexpressionalone leads to sequestration of endogenous dynamininto inactive complexes, but when the two proteins areexpressed together, forming a heterodimer, endocyto-sis -
Abstract_Book_HMM_v2
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/conferences/JM_Abstract_Book.pdf15 Sep 2011: To overcome problems caused by protein overexpression, our laboratory has used Zinc Finger Nuclease (ZFN) technology to express RFP- and GFP-tagged versions of clathrin and dynamin, respectively, preserving their endogenous -
doi:10.1016/j.devcel.2005.09.001
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/37_Shaffer_KL_DevCell_2005.pdf7 Oct 2005: he same for both Crt and Prl-Crt and were comparableo the levels of endogenous Crt (Figure 5A). ... A) Crt/ cells stably expressing either Crt-GFP or Prl-Crt-GFPwere analyzed (in duplicate) by immunoblotting with anti-Crt andanti-Sec61β (an endogenous -
Vol. 9 (2012), Number 1 | Jg. 9 (2012), ...
https://www.robinson.cam.ac.uk/postkeynesian/downloads/Pusch/TP131112.pdf9 May 2012: Hansen, New York: W.W. Norton, 135 – 155. Setterfield, M. (2006): Effective demand and endogenous money in a path-dependent economy: Towards a ›Moorian‹ credit supply curve – and a reconciliation between ... ed.), Complexity, Endogenous Money and -
2168 | K. von der Malsburg et al. Molecular ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/93_vonderMalsburg-_K_MBC_2015.pdf11 Aug 2015: Because stable association of NEMF with 60S requires a peptidyl-tRNA (Shao et al., 2015), these complexes are likely to contain endogenous nascent chains. ... We examined this idea by analysis of endogenous RNCs on ER microsomes isolated from native -
Molecular Biology of the CellVol. 21, 4325– 4337, December ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/67_Emerman_AE_MBC_2010.pdf11 Nov 2011: This indicated that BirA has no detectable targetsendogenous to mammalian cells, and that the BioTag is notmodified by endogenous enzymes. ... Note that lowlevels of endogenous PrP are visualized at 35kDa in the SS-PrP samples probed with-PrP. -
70 88..91
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/97_Voorhees_RM_Science_2016.pdf5 Jan 2016: ribosomes used for the cryo-tomography structure (16), thepresence or absence of heterogeneous endogenous nascentpolypeptides within Sec61 could not be determined conclusively.Thus, the conclusion that the Sec61 complex is constitutivelyopen -
elife.elifesciences.org MacKinnon et al. eLife 2014;3:e01483. DOI:…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/83_MacKinnon_AL_eLife_2014.pdf9 Sep 2015: Upon induction with tetracycline, both wild-type and mutant Sec61 transgenes (untagged) were expressed at similar levels as the endogenous protein (Figure 6—figure supplement 1C). ... Because we could not easily distinguish endogenous Sec61 from the -
The EMBO Journal Vol.17 No.18 pp.5273–5285, 1998 Crystal structure ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Owen_McMahon1998.pdf9 Sep 1998: We believe this effectis due to sequestration of endogenous dynamin away fromclathrin-coated pits. -
nrm2162.indd
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17450176.pdf14 Jun 2007: Many plasma-membrane proteins are in a constant flux throughout the internal trafficking pathways of the cell. Some receptors, such as that for transferrin, are continuously internalized into recycling endosomes and returned to the cell surface. -
SISTEMA NACIONAL DE INOVAÇÕES, COMPETITIVIDADE E CRESCIMENTO DAS…
https://www.robinson.cam.ac.uk/postkeynesian/downloads/Resende/MR070513.pdf4 Mar 2013: considered as a determinant of savings. It is endogenous and passively adjusts to produce. -
Sraffa-Keynes 3-2 (March 2012)
https://www.robinson.cam.ac.uk/postkeynesian/downloads/Park/MSP130312v2.pdf7 Mar 2012: 1. Sraffa’s ‘Given’ Quantities of Output and Keynes’s Principle of Effective Demand. Man-Seop Park. Korea University manseop@korea.ac.kr. [I]t is in the ‘present’ that the ‘normal’ rate of profits has always been firmly located. … -
elifesciences.org Szwedziak et al. eLife 2014;3:e04601. DOI:…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsZ_Piotr_eLife_2014.pdf31 Dec 2014: elifesciences.org. Szwedziak et al. eLife 2014;3:e04601. DOI: 10.7554/eLife.04601 1 of 22. Architecture of the ring formed by the tubulin homologue FtsZ in bacterial cell divisionPiotr Szwedziak†, Qing Wang†, Tanmay A M Bharat, Matthew Tsim, Jan -
Estere Seinkmane – November 2018 1 5. Metabolism throughout ...
https://www.murrayedwards.cam.ac.uk/sites/default/files/files/Activity%205_%20Metabolism%20throughout%20the%20day_Solutions.pdf5 Feb 2019: and Scheer, F. Endogenous circadian system and circadian misalignment impact glucose tolerance via separate mechanisms in humans. ... The circadian clock is an endogenous biological mechanism that drives roughly 24h cycles in various physiological -
d402_16g 822..826
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/15_Hegde_RS_Nature_1999.pdf9 Dec 1999: Trans-genic (Tg) mice expressing each of these mutant PrPs in the FVB/Pmp0/0 background (null for the endogenous PrP gene) weregenerated and characterized (see Table 1 and Fig.
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